Construct: ORF TRCN0000474831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005990.1_s317c1
- Derived from:
- ccsbBroadEn_05051
- DNA Barcode:
- ACTGATAGTACCAGCTTAGCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC16A14 (151473)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474831
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151473 | SLC16A14 | solute carrier family 16 me... | NM_152527.5 | 100% | 100% | |
| 2 | human | 151473 | SLC16A14 | solute carrier family 16 me... | XM_011510751.3 | 100% | 100% | |
| 3 | human | 151473 | SLC16A14 | solute carrier family 16 me... | XM_017003480.2 | 100% | 100% | |
| 4 | human | 151473 | SLC16A14 | solute carrier family 16 me... | XM_011510752.2 | 91.5% | 91.1% | (many diffs) |
| 5 | human | 151473 | SLC16A14 | solute carrier family 16 me... | XM_005246353.4 | 90.5% | 90.5% | 258_259ins144 |
| 6 | mouse | 71781 | Slc16a14 | solute carrier family 16 (m... | NM_027921.1 | 81.9% | 87.1% | (many diffs) |
| 7 | mouse | 71781 | Slc16a14 | solute carrier family 16 (m... | XM_006496551.3 | 81.9% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1596
- ORF length:
- 1530
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta taccagtcat gaagatattg ggtatgattt tgaagatggc cccaaagaca 121 aaaagacact gaagccccac ccaaacattg atggcggatg ggcttggatg atggtgctct 181 cctctttctt tgtgcacatc ctcatcatgg gctcccagat ggccctgggt gtcctcaacg 241 tggaatggct ggaagaattc caccagagcc gcggcctgac cgcctgggtc agctccctca 301 gcatgggcat caccttgata gtgggccctt tcatcggctt gttcattaac acctgtgggt 361 gccgccagac tgcgatcatt ggagggctcg tcaactccct gggctgggtg ttgagtgcct 421 atgctgcaaa cgtgcattat ctcttcatta cttttggagt cgcagctggc ctgggcagcg 481 ggatggccta cctgccagcg gtggtcatgg tgggcaggta tttccagaag agacgcgccc 541 tcgcccaggg cctcagcacc acggggaccg gattcggtac gttcctaatg actgtgctgc 601 tgaagtacct gtgcgcagag tacggctgga ggaatgccat gttgatccaa ggtgccgttt 661 ccctaaacct gtgtgtttgt ggggcgctca tgaggcccct ctctcctggt aaaaacccaa 721 acgacccagg agagaaagat gtgcgtggcc tgccagcgca ctccacagaa tctgtgaagt 781 caactggaca gcagggaaga acagaagaga aggatggtgg gctcgggaac gaggagaccc 841 tctgcgacct gcaagcccag gagtgccccg atcaggccgg gcacaggaag aacatgtgtg 901 ccctccggat tctgaagact gtcagctggc tcaccatgag agtcaggaag ggcttcgagg 961 actggtattc gggctacttt gggacagcct ctctatttac aaatcgaatg tttgtagcct 1021 ttattttctg ggctttgttt gcatacagca gctttgtcat ccccttcatt cacctcccag 1081 aaatcgtcaa tttgtataac ttatcggagc aaaacgacgt tttccctctg acgtcaatta 1141 tagcaatagt tcacatcttt ggaaaagtga tcctgGGCGT CATAGCCGAC TTGCCTTGCA 1201 TTAGTGTTTG GAATGTCTTC CTGTTGGCCA ACTTCACCCT TGTCCTCAGT ATTTTTATTC 1261 TGCCGTTGAT GCACACGTAC GCTGGCCTGG CGGTCATCTG TGCGCTGATA GGGTTTTCCA 1321 GTGGTTATTT CTCCCTAATG CCCGTAGTGA CTGAAGACTT GGTTGGCATT GAACACCTGG 1381 CCAATGCCTA CGGCATCATC ATCTGTGCTA ATGGCATCTC TGCATTGCTG GGACCACCTT 1441 TTGCAGGGTG GATCTATGAC ATCACGCAAA AATATGATTT TTCCTTCTAC ATATGTGGTT 1501 TGCTTTACAT GATAGGAATA CTCTTTTTAC TTATTCAGCC GTGCATTCGA ATTATAGAAC 1561 AATCCAGAAG AAAATACATG GATGGTGCAC ATGTTTACCC AACTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 AACTGATAGT ACCAGCTTAG CAGTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt