Construct: ORF TRCN0000474841
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011406.1_s317c1
- Derived from:
- ccsbBroadEn_02200
- DNA Barcode:
- AGGTGTTCTGTGTGTATCATATAT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOSR2 (9570)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474841
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_004287.4 | 100% | 100% | |
| 2 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001353114.1 | 99.5% | 99.5% | 94_95insTAG |
| 3 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_054022.4 | 93.7% | 85.5% | (many diffs) |
| 4 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_011525501.3 | 92.4% | 91.5% | (many diffs) |
| 5 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_011525502.3 | 92.2% | 91.5% | 584C>G;585_586insGGG;588_589ins45 |
| 6 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001363851.1 | 91.5% | 91.5% | 0_1ins54 |
| 7 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025389.1 | 91.5% | 91.5% | 0_1ins54 |
| 8 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_006722190.4 | 90.2% | 86.6% | (many diffs) |
| 9 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025383.2 | 88.6% | 78% | (many diffs) |
| 10 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001012511.2 | 82.5% | 77.1% | (many diffs) |
| 11 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001321133.2 | 80.8% | 71.4% | (many diffs) |
| 12 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025378.1 | 80.4% | 71% | (many diffs) |
| 13 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001330252.1 | 77.8% | 77.8% | 336_337ins141 |
| 14 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001353115.1 | 77.3% | 77.3% | 94_95insTAG;333_334ins141 |
| 15 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025379.1 | 73.8% | 64.8% | (many diffs) |
| 16 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025380.1 | 73.8% | 64.8% | (many diffs) |
| 17 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025381.1 | 73.4% | 64.4% | (many diffs) |
| 18 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025382.1 | 73.4% | 64.4% | (many diffs) |
| 19 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001353116.2 | 71.6% | 64.6% | (many diffs) |
| 20 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NM_001321134.1 | 69.3% | 69.3% | 0_1ins54;282_283ins141 |
| 21 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025395.1 | 69.3% | 69.3% | 0_1ins54;282_283ins141 |
| 22 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025392.1 | 67.8% | 65.5% | (many diffs) |
| 23 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025386.1 | 62.5% | 54.2% | (many diffs) |
| 24 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025387.1 | 62.1% | 53.8% | (many diffs) |
| 25 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XM_017025394.1 | 60% | 57.7% | (many diffs) |
| 26 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XR_934619.3 | 47.2% | (many diffs) | |
| 27 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XR_002958092.1 | 34.4% | (many diffs) | |
| 28 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NR_148351.2 | 26.9% | (many diffs) | |
| 29 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XR_001752696.2 | 26.8% | (many diffs) | |
| 30 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NR_148349.2 | 26.6% | (many diffs) | |
| 31 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XR_002958091.1 | 24.7% | (many diffs) | |
| 32 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XR_002958093.1 | 22.1% | (many diffs) | |
| 33 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | NR_148350.2 | 20.5% | (many diffs) | |
| 34 | human | 9570 | GOSR2 | golgi SNAP receptor complex... | XR_934616.3 | 15.8% | (many diffs) | |
| 35 | mouse | 56494 | Gosr2 | golgi SNAP receptor complex... | NM_019650.3 | 88.8% | 88.2% | (many diffs) |
| 36 | mouse | 56494 | Gosr2 | golgi SNAP receptor complex... | XM_006533802.2 | 88.3% | 87.7% | (many diffs) |
| 37 | mouse | 56494 | Gosr2 | golgi SNAP receptor complex... | XM_006533803.2 | 69.1% | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcccctgttc cagcaaacgc acaagcaggt ccacgagatc cagtcttgca 121 tgggacgcct ggagacggca gacaagcagt ctgtgcacat agtagaaaac gaaatccaag 181 caagcataga ccagatattc agccgtctag aacgtctgga gattttgtcc agcaaggagc 241 cccctaacaa aaggcaaaat gCCAGACTTC GGGTTGACCA GTTAAAGTAT GATGTCCAGC 301 ACCTGCAGAC TGCGCTCAGA AACTTCCAGC ATCGGCGCCA TGCAAGGGAG CAGCAGGAGA 361 GACAGCGAGA AGAGCTTCTG TCTCGAACCT TCACCACTAA CGACTCTGAC ACCACCATAC 421 CAATGGACGA ATCACTGCAG TTTAACTCCT CCCTCCAGAA AGTTCACAAC GGCATGGATG 481 ACCTCATTTT AGATGGGCAC AATATTTTAG ATGGACTGAG GACCCAGAGA CTGACCTTGA 541 AGGGGACTCA GAAGAAGATC CTTGACATTG CCAACATGCT GGGCTTGTCC AACACAGTGA 601 TGCGGCTCAT CGAGAAGCGG GCTTTCCAGG ACAAGTACTT TATGATAGGT GGGATGCTGC 661 TGACCTGTGT GGTCATGTTC CTCGTGGTGC AGTACCTGAC ATCCCAACTT TCTTGTACAA 721 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 781 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 841 GGACGAAGGT GTTCTGTGTG TATCATATAT ACGCGTTAAG TCgacaatca acctctggat 901 tacaaaattt gtgaaagatt