Construct: ORF TRCN0000474845

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002114.4_s317c1
Derived from:
ccsbBroadEn_14932
DNA Barcode:
GGCTCCACACATCCACTGCTTAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AURKB (9212)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474845
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9212 AURKB aurora kinase B NM_001313950.2 100% 100%
2 human 9212 AURKB aurora kinase B NM_004217.4 100% 100%
3 human 9212 AURKB aurora kinase B NM_001284526.2 99.7% 99.7% 207_209delCAG
4 human 9212 AURKB aurora kinase B NM_001313953.3 88.9% 87.2% (many diffs)
5 human 9212 AURKB aurora kinase B NM_001256834.3 88% 88% 0_1ins123
6 human 9212 AURKB aurora kinase B NM_001313951.1 88% 88% 0_1ins123
7 human 9212 AURKB aurora kinase B XM_011524072.3 88% 88% 0_1ins123
8 human 9212 AURKB aurora kinase B XM_017025307.2 88% 88% 0_1ins123
9 human 9212 AURKB aurora kinase B NM_001313952.2 87.8% 87.8% 0_1ins123;84_86delCAG
10 human 9212 AURKB aurora kinase B XM_017025308.2 77% 75.2% (many diffs)
11 human 9212 AURKB aurora kinase B NR_132731.1 74.6% (many diffs)
12 human 9212 AURKB aurora kinase B NR_132730.2 71.6% 1_71del;327_328ins142;962_1101del
13 human 9212 AURKB aurora kinase B NM_001313954.2 54.3% 49.2% 0_1ins466;71_80delGGTCCGGGCG
14 human 9212 AURKB aurora kinase B XM_017025309.1 54.3% 49.2% 0_1ins466;71_80delGGTCCGGGCG
15 human 9212 AURKB aurora kinase B XM_017025310.1 54.3% 49.2% 0_1ins466;71_80delGGTCCGGGCG
16 human 9212 AURKB aurora kinase B XM_017025311.1 54.3% 49.2% 0_1ins466;71_80delGGTCCGGGCG
17 human 9212 AURKB aurora kinase B NM_001313955.2 50.2% 47.5% (many diffs)
18 mouse 20877 Aurkb aurora kinase B NM_011496.2 81.3% 82.9% (many diffs)
19 mouse 20877 Aurkb aurora kinase B XM_006532725.3 53.5% 55% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1098
ORF length:
1032
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ccagaaggag aactcctacc cctggcccta cggccgacag acggctccat
121ctggcctgag caccctgccc cagcgagtcc tccggaaaga gcctgtcacc ccatctgcac
181ttgtcctcat gagccgctcc aatgtccagc ccacagctgc ccctggccag aaggtgatgg
241agaatagcag tgggacaccc gacatcttaa cgcggcactt cacaattgat gactttgaga
301ttgggcgtcc tctgggcaaa ggcaagtttg gaaacgtgta cttggctcgg gagaagaaaa
361gccatttcat cgtggcgctc aaggtcctct tcaagtccca gatagagaag gagggcgtgg
421agcatcagct gcgcagagag atcgaaatcc aggcccacct gcaccatccc aacatcctgc
481gtctctacaa ctatttttat gaccggagga ggatctactt gattctagag tatgcccccc
541gcggggagct ctacaaggag ctgcagaaga gctgcacatt tgacgagcag cgaacagcca
601CGATCATGGA GGAGTTGGCA GATGCTCTAA TGTACTGCCA TGGGAAGAAG GTGATTCACA
661GAGACATAAA GCCAGAAAAT CTGCTCTTAG GGCTCAAGGG AGAGCTGAAG ATTGCTGACT
721TCGGCTGGTC TGTGCATGCG CCCTCCCTGA GGAGGAAGAC AATGTGTGGC ACCCTGGACT
781ACCTGCCCCC AGAGATGATT GAGGGGCGCA TGCACAATGA GAAGGTGGAT CTGTGGTGCA
841TTGGAGTGCT TTGCTATGAG CTGCTGGTGG GGAACCCACC CTTTGAGAGT GCATCACACA
901ACGAGACCTA TCGCCGCATC GTCAAGGTGG ACCTAAAGTT CCCCGCTTCC GTGCCCATGG
961GAGCCCAGGA CCTCATCTCC AAACTGCTCA GGCATAACCC CTCGGAACGG CTGCCCCTGG
1021CCCAGGTCTC AGCCCACCCT TGGGTCCGGG CCAACTCTCG GAGGGTGCTG CCTCCCTCTG
1081CCCTTCAATC TGTCGCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1141TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1201GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCTCCA CACATCCACT
1261GCTTAGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)