Construct: ORF TRCN0000474847
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018859.2_s317c1
- Derived from:
- ccsbBroadEn_15104
- DNA Barcode:
- ATTTAGCCGTGCCCAGATTTCCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BMP2K (55589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474847
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55589 | BMP2K | BMP2 inducible kinase | NM_017593.4 | 97.2% | 60.5% | (many diffs) |
| 2 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_011532102.1 | 92.6% | 57.9% | (many diffs) |
| 3 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_005263117.1 | 56.6% | 35.6% | (many diffs) |
| 4 | human | 55589 | BMP2K | BMP2 inducible kinase | NM_198892.1 | 55.2% | 34.5% | (many diffs) |
| 5 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_017008381.1 | 50.3% | 29.3% | (many diffs) |
| 6 | human | 55589 | BMP2K | BMP2 inducible kinase | XM_017008382.1 | 31.4% | 10.4% | (many diffs) |
| 7 | mouse | 140780 | Bmp2k | BMP2 inducible kinase | XM_006534789.3 | 84.3% | 57.9% | (many diffs) |
| 8 | mouse | 140780 | Bmp2k | BMP2 inducible kinase | XM_006534788.3 | 77.7% | 52.8% | (many diffs) |
| 9 | mouse | 140780 | Bmp2k | BMP2 inducible kinase | NM_080708.1 | 49% | 33% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1281
- ORF length:
- 1212
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagaagttc tctcggatgc ccaagtcgga gggcggcagc ggcggcggag 121 cggcgggcgg ctcgtccgtg ggggtccggg tgttcgcggt cggccgccac caggtcaccc 181 tggaagagtc gctggccgaa ggtggattct ccacagtttt cctcgtgcgt actcacggtg 241 gaatccgatg tgcattgaag cgaatgtatg tcaataacat gccagacctc aatgtttgta 301 aaagggaaat tacaattatg aaagagctat ctggtcacaa aaatattgtg ggctatttgg 361 actgtgctgt taattcaatt agtgataatg tatgggaagt ccttatctta atggaatatt 421 gtcgagctgg acaggtagtg aatcaaatga ataagaagct acagacgggt tttacagaac 481 cagaagtgtt acagatattc tgtgatacct gtgaagctgt tgcaaggttg catcagtgta 541 agactccaat aattcaccgg gatctgaagg tagaaaatat tttgttgaat gatggtggga 601 actatgtact ttgtgacttt ggcagtgcca ctaataaatt tcttaatcct caaaaagatg 661 gagttaatgt agtagaagaa gaaattaaaa agtatacaac tctgtcatac agagcccctg 721 aaatgatcaa cctttatgga gggaaaccca tcaccaccaa ggctgatatc tgggcactgg 781 gatgtctact ctataaactt tgtttcttca ctcttccttt tggtgagagt caggttgcta 841 tctgtgatgg caacttcacc atcccagaca attctcgtta ctcccgtaac atacattgct 901 taataaggtt catgcttgaa ccagatccgg aacatagacc tgatatattt caagtgtcat 961 attttgcatt taaatttgcc aaaaaggatt gtccagtctc caacatcaat aattcttcta 1021 ttccttcagc tcttcctgaa ccgatggctg ctagtgaagc agctgctagg aaaagccaaa 1081 taaaagccag aataacagat accattggac caacagaaac ctcaattgca ccaagacaaa 1141 gaccaaaggc caactctgct actactgcca ctcccagtgt gctgaccatt caaagttcag 1201 caacacctgt taaagtcctt gctcctggtg aattcagtaa ccatagacca aaaggggcac 1261 taagacctgg aaaagggccc tgaaaattta ttggttcagg gaactcctca gcagccgcca 1321 cagcagcata gagtactcca gcaactacag cagggagatt ggagattaca gcaactccca 1381 tttacagcat cgtcatcctc accagcagca gcagcagcag cagcagcaac agcaacagca 1441 gcagcagcaa cagcaacagc agcagcagca gcagcagcag cagcaccacc acccaccacc 1501 accaccacct acttcaagat gcttatatgc agcagtatca acatgcaaca cagcagcaac 1561 agatgcttca acaacaattt ttaatgcatt cggtatatca accacaaccT TCTGCATCAC 1621 AGTATCCTAC AATGATGCCG CAGTATCAGC AGGCTTTCTT TCAACAGCAG ATGCTAGCTC 1681 AACATCAGCC GTCTCAACAA CAGGCATCAC CTGAATATCT TACCTCCCCT CAAGAGTTCT 1741 CACCAGCCTT AGTTTCCTAC ACTTCATCAC TTCCAGCTCA GGTTGGAACC ATAATGGACT 1801 CCTCCTATAG TGCCAATAGG TCAGTTGCTG ATAAAGAGGC CATTGCAAAT TTCACAAATC 1861 AGAAGAACAT CAGCAATCCA CCTGATATGT CAGGGTGGAA TCCTTTTGGA GAGGATAATT 1921 TCTCTAAGTT AACAGAAGAG GAACTATTGG ACAGAGAATT TGACCTTCTA AGATCAAGTA 1981 AGGGACACTT GAAGGCTTAT TTTGCTTCAC AATTGCCAAC TTTCTTGTAC AAAGTGGTTG 2041 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2101 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 2161 TTAGCCGTGC CCAGATTTCC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2221 ttgtgaaaga tt