Construct: ORF TRCN0000474853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001936.1_s317c1
- Derived from:
- ccsbBroadEn_09370
- DNA Barcode:
- AGCACGGCACACCGTCACAGTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BIRC8 (112401)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 331 | XIAP | X-linked inhibitor of apopt... | NM_001167.3 | 59.2% | 55.1% | (many diffs) |
2 | human | 331 | XIAP | X-linked inhibitor of apopt... | NM_001204401.1 | 59.2% | 55.1% | (many diffs) |
3 | human | 331 | XIAP | X-linked inhibitor of apopt... | XM_006724754.2 | 59.2% | 55.1% | (many diffs) |
4 | human | 331 | XIAP | X-linked inhibitor of apopt... | XM_011531329.2 | 59.2% | 55.1% | (many diffs) |
5 | human | 112401 | BIRC8 | baculoviral IAP repeat cont... | NR_159880.1 | 55.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1080
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tagtgaagaa gctagattac agtcgtttca caactggcca gcctctgccc 121 acttgacccc gagagagctg gccagtgctg ggctgtacta cacaggcact gatgaccaag 181 tgcagtgctt ctgttgtggc ggaaaactga aaaactggga acctggtgat cgtgcctggt 241 cagaacacag gagacatttt cctaattgct tctttatttt gggccacaac gttaatattc 301 gaggtgaatc tgatgttgcg agttctgata ggaatttctc aaattcaaca agttctccaa 361 ggaatccatc catgacgggt tatgaagccc ggctcattac ttttgggaca tggatgtact 421 ccgtcaacaa agagcagctt gcaagagctg gattttatgc tataggtcaa gaggataaag 481 tacagtgctt tcactgtgga ggagggctag ccaactggaa gcccaaggaa gatccttggg 541 aacagcatgc taaatggtat ccaggttgca aatatcTGCT AGAAGAGAAG GGACATGAAT 601 ATATAAACAA CATTCATTTA ACCCGTTCAC TTGAGGGAGC TCTGGTACAA ACTACCAAGA 661 AAACACCATC ACTAACTAAA AGAATCAGTG ATACCATCTT CCCTAATCCT ATGCTACAAG 721 AAGCTATACG AATGGGATTT GATTTCAAGG ACGTTAAGAA AATAATGGAG GAAAGAATTC 781 AAACATCTGG GAGCAACTAT AAAACGCTTG AGGTTCTTGT TGCAGATCTA GTGAGCGCTC 841 AGAAAGACAC TACAGAAAAT GAATTGAATC AGACTTCATT GCAGAGAGAA ATCAGCCCTG 901 AAGAGCCGCT AAGGCGTCTG CAAGAGGAGA AGCTTTGTAA AATCTGCATG GACAGATATA 961 TCGCTGTTGT TTTTATTCCT TGTGGACATC TGGTCACTTG TAAACAATGT GCTGAAGCAG 1021 TTGACAGATG TCCCATGTGC AGCGCGGTTA TTGATTTCAA GCAAAGAGTT TTTATGTCTT 1081 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1141 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1201 TTTATATATC TTGTGGAAAG GACGAAGCAC GGCACACCGT CACAGTTATA CGCGTTAAGT 1261 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt