Construct: ORF TRCN0000474867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003950.1_s317c1
- Derived from:
- ccsbBroadEn_04371
- DNA Barcode:
- TGCCAAACACAACCTCCGGGTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT16L1 (84309)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474867
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84309 | NUDT16L1 | nudix hydrolase 16 like 1 | NM_032349.3 | 100% | 100% | |
| 2 | human | 84309 | NUDT16L1 | nudix hydrolase 16 like 1 | NM_001370585.1 | 99% | 99% | 152_153insGATGCA |
| 3 | human | 84309 | NUDT16L1 | nudix hydrolase 16 like 1 | NM_001370587.1 | 81% | 81% | 412_413ins120 |
| 4 | human | 84309 | NUDT16L1 | nudix hydrolase 16 like 1 | NM_001193452.1 | 71.9% | 67.8% | 414_483del;576_577ins127 |
| 5 | human | 84309 | NUDT16L1 | nudix hydrolase 16 like 1 | NM_001370586.1 | 71.1% | 66.9% | 152_153insGATGCA;408_477del;570_571ins127 |
| 6 | human | 84309 | NUDT16L1 | nudix hydrolase 16 like 1 | XM_005255633.5 | 63.3% | 50.8% | (many diffs) |
| 7 | human | 84309 | NUDT16L1 | nudix hydrolase 16 like 1 | NM_001370588.1 | 58.7% | 58.7% | 152_153ins261 |
| 8 | mouse | 66911 | Nudt16l1 | nudix (nucleoside diphospha... | NM_025839.4 | 85.4% | 95.7% | (many diffs) |
| 9 | mouse | 66911 | Nudt16l1 | nudix (nucleoside diphospha... | NM_001316715.1 | 61.7% | 66.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gacggcggcg gttccggagc tgaagcagat cagccgggtg gaggcgatgc 121 gcctagggcc gggctggagc cactcgtgcc acgccatgct gtacgccgcc aaccctgggc 181 agcTCTTCGG CCGCATCCCC ATGCGCTTCT CGGTGCTGAT GCAGATGCGT TTCGACGGGC 241 TGCTGGGCTT CCCCGGGGGC TTCGTGGACC GGCGCTTCTG GTCGCTGGAG GACGGCCTGA 301 ACCGGGTGCT GGGCCTGGGC CTGGGCTGCC TGCGCCTCAC CGAGGCCGAC TACCTGAGCT 361 CGCACCTGAC CGAGGGCCCA CACCGCGTCG TGGCGCACCT GTACGCGCGG CAGCTGACGC 421 TGGAGCAGCT GCACGCCGTG GAGATCAGCG CGGTGCACTC GCGCGACCAC GGCCTGGAGG 481 TGCTGGGCCT CGTGCGGGTC CCGCTGTACA CCCAGAAGGA CCGAGTCGGA GGCTTCCCCA 541 ACTTCCTGAG CAACGCCTTC GTGAGCACGG CTAAGTGCCA GCTCCTCTTT GCCCTCAAGG 601 TGCTCAACAT GATGCCCGAG GAGAAGCTGG TTGAGGCCCT GGCTGCAGCC ACCGAGAAGC 661 AGAAGAAGGC CCTGGAGAAG TTGCTCCCGG CCTCCTCTTG CCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGATGCCAA ACACAACCTC CGGGTTAAAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt