Construct: ORF TRCN0000474885

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010565.1_s317c1
Derived from:
ccsbBroadEn_03427
DNA Barcode:
TCCCGTCAATATCTAATATACCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HMGCLL1 (54511)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474885
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... NM_001042406.1 100% 100%
2 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... NM_019036.2 91.8% 91.8% 108_197del
3 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... NM_001287741.1 90.5% 90.5% 297_298ins96
4 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... XM_011514700.3 78.3% 78.2% 796_798delACC;803_804delTCinsGA;807_808ins216
5 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... NM_001287746.1 70.8% 70.8% 0_1ins297
6 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... XM_024446469.1 70.8% 70.8% 0_1ins297
7 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... NM_001287753.1 69.7% 69.7% 297_298ins309
8 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... XM_024446470.1 52.6% 52.6% 0_1ins483
9 human 54511 HMGCLL1 3-hydroxymethyl-3-methylglu... XM_024446471.1 52.6% 52.6% 0_1ins483
10 mouse 208982 Hmgcll1 3-hydroxymethyl-3-methylglu... NM_173731.2 87.6% 91.8% (many diffs)
11 mouse 208982 Hmgcll1 3-hydroxymethyl-3-methylglu... XM_006510947.3 75.9% 79.8% (many diffs)
12 mouse 208982 Hmgcll1 3-hydroxymethyl-3-methylglu... XM_006510948.3 70.8% 74.9% (many diffs)
13 mouse 208982 Hmgcll1 3-hydroxymethyl-3-methylglu... XM_011242696.2 68.6% 71.7% (many diffs)
14 mouse 208982 Hmgcll1 3-hydroxymethyl-3-methylglu... XM_006510949.3 67.9% 71.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1086
ORF length:
1020
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gaatgtgcca tccgcggtga agcactgcct cagctaccag cagcttctcc
121gggagcatct ctggatcggg gattcagtgg caggggcgct cgaccccgcg caggaaacat
181cccagttatc tggactccct gagtttgtta aaatagtaga agttgggcct agggatggat
241tgcagaatga aaaggttata gttcctacag atataaaaat tgaatttatc aatcgacttt
301cccaaactgg cttgtctgta atagaagtga ctagctttgt gtcttccaga tgggtaccac
361agatggctga tcacactgaa gtaatgaaag gcattcatca atatccagga gttcgctatc
421ctgtccttac tcctaatctt cagggttttc accatgctgt tgctgctgga gctactgaga
481tatcagtttt tggagctgca tctgaatcct ttagcaagaa gaatattaac tgttccattg
541aagaaagtat gggaaaattt gaggaggttg ttaagtcTGC AAGACACATG AATATTCCAG
601CACGAGGGTA TGTGTCTTGT GCTCTGGGCT GTCCATATGA AGGAAGTATT ACACCGCAAA
661AAGTGACAGA AGTGTCTAAG AGATTGTACG GCATGGGTTG TTATGAGATC TCTCTAGGAG
721ACACAATTGG AGTGGGAACT CCAGGAAGTA TGAAAAGAAT GTTGGAAAGT GTGATGAAAG
781AAATCCCACC AGGTGCTCTT GCTGTTCACT GTCATGACAC ATACGGACAA GCCTTAGCAA
841ATATCCTTAC GGCCCTTCAG ATGGGAATTA ATGTGGTGGA CTCCGCAGTA TCCGGATTAG
901GTGGCTGCCC TTATGCAAAA GGTGCTTCTG GGAATGTAGC CACTGAGGAT TTGATATATA
961TGCTTAATGG CCTGGGGCTC AATACAGGTG TGAATCTATA CAAAGTGATG GAAGCTGGTG
1021ACTTTATTTG CAAAGCTGTG AATAAAACCA CAAACTCTAA AGTAGCACAA GCCTCCTTCA
1081ATGCTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1141TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1201CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCCGTCAA TATCTAATAT ACCACACGCG
1261TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)