Construct: ORF TRCN0000474885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010565.1_s317c1
- Derived from:
- ccsbBroadEn_03427
- DNA Barcode:
- TCCCGTCAATATCTAATATACCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGCLL1 (54511)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474885
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | NM_001042406.1 | 100% | 100% | |
| 2 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | NM_019036.2 | 91.8% | 91.8% | 108_197del |
| 3 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | NM_001287741.1 | 90.5% | 90.5% | 297_298ins96 |
| 4 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | XM_011514700.3 | 78.3% | 78.2% | 796_798delACC;803_804delTCinsGA;807_808ins216 |
| 5 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | NM_001287746.1 | 70.8% | 70.8% | 0_1ins297 |
| 6 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | XM_024446469.1 | 70.8% | 70.8% | 0_1ins297 |
| 7 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | NM_001287753.1 | 69.7% | 69.7% | 297_298ins309 |
| 8 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | XM_024446470.1 | 52.6% | 52.6% | 0_1ins483 |
| 9 | human | 54511 | HMGCLL1 | 3-hydroxymethyl-3-methylglu... | XM_024446471.1 | 52.6% | 52.6% | 0_1ins483 |
| 10 | mouse | 208982 | Hmgcll1 | 3-hydroxymethyl-3-methylglu... | NM_173731.2 | 87.6% | 91.8% | (many diffs) |
| 11 | mouse | 208982 | Hmgcll1 | 3-hydroxymethyl-3-methylglu... | XM_006510947.3 | 75.9% | 79.8% | (many diffs) |
| 12 | mouse | 208982 | Hmgcll1 | 3-hydroxymethyl-3-methylglu... | XM_006510948.3 | 70.8% | 74.9% | (many diffs) |
| 13 | mouse | 208982 | Hmgcll1 | 3-hydroxymethyl-3-methylglu... | XM_011242696.2 | 68.6% | 71.7% | (many diffs) |
| 14 | mouse | 208982 | Hmgcll1 | 3-hydroxymethyl-3-methylglu... | XM_006510949.3 | 67.9% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaatgtgcca tccgcggtga agcactgcct cagctaccag cagcttctcc 121 gggagcatct ctggatcggg gattcagtgg caggggcgct cgaccccgcg caggaaacat 181 cccagttatc tggactccct gagtttgtta aaatagtaga agttgggcct agggatggat 241 tgcagaatga aaaggttata gttcctacag atataaaaat tgaatttatc aatcgacttt 301 cccaaactgg cttgtctgta atagaagtga ctagctttgt gtcttccaga tgggtaccac 361 agatggctga tcacactgaa gtaatgaaag gcattcatca atatccagga gttcgctatc 421 ctgtccttac tcctaatctt cagggttttc accatgctgt tgctgctgga gctactgaga 481 tatcagtttt tggagctgca tctgaatcct ttagcaagaa gaatattaac tgttccattg 541 aagaaagtat gggaaaattt gaggaggttg ttaagtcTGC AAGACACATG AATATTCCAG 601 CACGAGGGTA TGTGTCTTGT GCTCTGGGCT GTCCATATGA AGGAAGTATT ACACCGCAAA 661 AAGTGACAGA AGTGTCTAAG AGATTGTACG GCATGGGTTG TTATGAGATC TCTCTAGGAG 721 ACACAATTGG AGTGGGAACT CCAGGAAGTA TGAAAAGAAT GTTGGAAAGT GTGATGAAAG 781 AAATCCCACC AGGTGCTCTT GCTGTTCACT GTCATGACAC ATACGGACAA GCCTTAGCAA 841 ATATCCTTAC GGCCCTTCAG ATGGGAATTA ATGTGGTGGA CTCCGCAGTA TCCGGATTAG 901 GTGGCTGCCC TTATGCAAAA GGTGCTTCTG GGAATGTAGC CACTGAGGAT TTGATATATA 961 TGCTTAATGG CCTGGGGCTC AATACAGGTG TGAATCTATA CAAAGTGATG GAAGCTGGTG 1021 ACTTTATTTG CAAAGCTGTG AATAAAACCA CAAACTCTAA AGTAGCACAA GCCTCCTTCA 1081 ATGCTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCCGTCAA TATCTAATAT ACCACACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt