Construct: ORF TRCN0000474910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016874.1_s317c1
- Derived from:
- ccsbBroadEn_10935
- DNA Barcode:
- TTTTTTGAAATTTAATGCATTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF2A (3796)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474910
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3796 | KIF2A | kinesin family member 2A | NM_001243952.1 | 98.9% | 98.9% | 1_21del |
| 2 | human | 3796 | KIF2A | kinesin family member 2A | NM_004520.4 | 96.1% | 96.1% | 1_81del |
| 3 | human | 3796 | KIF2A | kinesin family member 2A | NM_001243953.1 | 93.4% | 93.3% | 1_81del;400_401ins57 |
| 4 | human | 3796 | KIF2A | kinesin family member 2A | NM_001098511.2 | 91.2% | 91.2% | 1_81del;1645_1758del |
| 5 | human | 3796 | KIF2A | kinesin family member 2A | XR_001742064.2 | 46.9% | (many diffs) | |
| 6 | mouse | 16563 | Kif2a | kinesin family member 2A | XM_006517539.3 | 89.1% | 94.9% | (many diffs) |
| 7 | mouse | 16563 | Kif2a | kinesin family member 2A | NM_001145779.1 | 88.2% | 94.1% | (many diffs) |
| 8 | mouse | 16563 | Kif2a | kinesin family member 2A | NM_008442.2 | 86.9% | 92.7% | (many diffs) |
| 9 | mouse | 16563 | Kif2a | kinesin family member 2A | XM_006517538.3 | 85.7% | 91.3% | (many diffs) |
| 10 | mouse | 16563 | Kif2a | kinesin family member 2A | XM_006517535.3 | 83.7% | 89.3% | (many diffs) |
| 11 | mouse | 16563 | Kif2a | kinesin family member 2A | XM_006517537.3 | 81.4% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2103
- ORF length:
- 2037
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt aacatcttta aatgaagata atgaaagtgt aactgttgaa tggatagaaa 121 atggagatac aaaaggcaaa gagattgacc tggagagcat cttttcactt aaccctgacc 181 ttgttcctga tgaagaaatt gaacccagtc cagaaacacc tccacctcca gcatcctcag 241 ccaaagtaaa caaaattgta aagaatcgac ggactgtagc ttctattaag aatgaccctc 301 cttcaagaga taatagagtg gttggttcag cacgtgcacg gcccagtcaa tttcctgaac 361 agtcttcctc tgcacaacag aatggtagtg tttcagatat atctccagtt caagctgcaa 421 aaaaggaatt tggaccccct tcacgtagaa aatctaattg tgtgaaagaa gtagaaaaac 481 tgcaagaaaa acgagagaaa aggagattgc aacagcaaga acttagagaa aaaagagccc 541 aggacgttga tgctacaaac ccaaattatg aaattatgtg tatgatcaga gactttagag 601 gaagtttgga ttatagacca ttaacaacag cagatcctat tgatgaacat aggatatgtg 661 tgtgtgtaag aaaacgacca ctcaataaaa aagaaactca aatgaaagat cttgatgtaa 721 tcacaattcc tagtaaagat gttgtgatgg tacatgaacc aaaacaaaaa gtagatttaa 781 caaggtacct agaaaaccaa acatttcgtt ttgattatgc ctttgatgac tcagctccta 841 atgaaatggt ttacaggttt actgctagac cactagtgga aactatattt gaaaggggaa 901 tggctacatg ctttgcttat gggcagactg gaagtggaaa aactcatact atgggtggtg 961 acttttcagg aaagaaccaa gattgttcta aaggaattta tgcattagca gctcgagatg 1021 tctttttaat gctaaagaag ccaaactata agaagctaga acttcaagta tatgcaacct 1081 tctttgaaat ttatagtgga aaggtgtttg acttgctaaa caggaaaaca aaattaagag 1141 ttctagaaga tggaaaacag caggttcaag tggtgggatt acaggaacgg gaggtcaaat 1201 gtgttgaaga tgtactgaaa ctcattgaca taggcaacag ttgcagaaca tccggtcaaa 1261 catctgcaaa tgcacattca tctcggagcc atgcagtgtt tcagattatt cttagaagga 1321 aaggaaaact acatggcaaa ttttctctca ttgatttggc tggaaatgaa agaggagctg 1381 atacttccag tgcggacagg caaactaggc ttgaaggtgc tgaaattaat aaaagccttt 1441 tagcactcaa ggagtgcatc agagccttag gtagaaataa acctcatact cctttccgtg 1501 caagtaaact cactcaggtg ttaagagatt ctttcatagg tgaaaactct cgtacctgca 1561 tgattgccac aatctctcca ggaatggcat cctgtgaaaa tactcttaat acattaagat 1621 atgcaaatag ggtcaaagaa ttgactgtag atccaactgc tgctggtgat gttcgtccaa 1681 taatgcacca tccaccaaac cagattgatg acttAGAGAC ACAGTGGGGT GTGGGGAGTT 1741 CCCCTCAGAG AGATGATCTA AAACTTCTTT GTGAACAAAA TGAAGAAGAA GTCTCTCCAC 1801 AGTTGTTTAC TTTCCACGAA GCTGTTTCAC AAATGGTAGA AATGGAAGAA CAAGTTGTAG 1861 AAGATCACAG GGCAGTGTTC CAGGAATCTA TTCGGTGGTT AGAAGATGAA AAGGCCCTCT 1921 TAGAGATGAC TGAAGAAGTA GATTATGATG TCGATTCATA TGCTACACAA CTTGAAGCTA 1981 TTCTTGAGCA AAAAATAGAC ATTTTAACTG AACTGCGGGA TAAAGTGAAA TCTTTCCGTG 2041 CAGCTCTACA AGAGGAGGAA CAAGCCAGCA AGCAAATCAA CCCGAAGAGA CCCCGTGCCC 2101 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2161 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2221 GGCTTTATAT ATCTTGTGGA AAGGACGATT TTTTGAAATT TAATGCATTT TAACGCGTTA 2281 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt