Construct: ORF TRCN0000474944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013923.1_s317c1
- Derived from:
- ccsbBroadEn_02891
- DNA Barcode:
- TCAATTTTGAGAGTCTCCACTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNKD (25953)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474944
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | NM_022572.4 | 100% | 100% | |
| 2 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | XM_017003772.1 | 91.9% | 91.9% | 707_708ins87 |
| 3 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | NM_015488.5 | 85.9% | 78.9% | (many diffs) |
| 4 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | XM_017003771.1 | 78.6% | 71.7% | (many diffs) |
| 5 | mouse | 56695 | Pnkd | paroxysmal nonkinesiogenic ... | NM_019999.2 | 88.9% | 91.9% | (many diffs) |
| 6 | mouse | 56695 | Pnkd | paroxysmal nonkinesiogenic ... | NM_001039509.1 | 79.5% | 75.9% | (many diffs) |
| 7 | mouse | 56695 | Pnkd | paroxysmal nonkinesiogenic ... | XM_006496167.3 | 77.1% | 65.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttggcagggc tggcccgcgg cgtggcagtg ggtcgccggc tgctggctcc 121 tcctcgtcct tgtcctcgtc ctacttgtga gcccccgcgg ctgccgagcg cggcggggcc 181 tccgcggtct gctcatggcg cacagccagc ggctgctctt ccgaatcggg tacagcctgt 241 acacccgcac ctggctcggg tacctcttct accgacagca gctgcgcagg gctcggaatc 301 gctaccctaa aggccactcg aaaacccagc cccgcctctt caatggagtg aaggtgcttc 361 ccatccctgt cctctcggac aactacagct acctcatcat cgacacccag gcccagctgg 421 ctgtggctgt ggacccttca gaccctcggg ctgtgcaggc ttccattgaa aaggaagggg 481 tcaccttggt cgccattctg tgtactcaca agcactggga ccacagtgga gggaaccgtg 541 acctcagccg gcggcaccgg gactgtcggg tgtacgggag ccctcaggac ggcatcccct 601 acctcaccca tcccctgtgt catcaagatg tggTCAGCGT GGGACGGCTT CAGATCCGGG 661 CCCTGGCTAC ACCTGGCCAC ACACAAGGCC ATCTGGTCTA CCTACTGGAT GGGGAGCCCT 721 ACAAGGGTCC CTCCTGCCTC TTCTCAGGGG ACCTGCTCTT CCTCTCTGGC TGTGGGCGGA 781 CCTTTGAGGG CAATGCAGAG ACCATGCTGA GCTCACTGGA CACTGTGCTG GGGCTAGGGG 841 ATGACACCCT TCTGTGGCCT GGTCATGAGT ATGCAGAGGA GAACCTGGGC TTTGCAGGTG 901 TGGTGGAGCC CGAGAACCTG GCCCGGGAGA GGAAGATGCA GTGGGTGCAG CGGCAGCGGC 961 TGGAGCGCAA GGGCACGTGC CCATCTACCC TGGGAGAGGA GCGCTCCTAC AACCCGTTCC 1021 TGAGAACCCA CTGCCTGGCG CTACAGGAGG CTCTGGGGCC GGGGCCGGGC CCCACTGGGG 1081 ATGATGACTA CTCCCGGGCC CAGCTCCTGG AAGAGCTCCG CCGGCTGAAG GATATGCACA 1141 AGAGCAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCAATT TTGAGAGTCT CCACTGTCAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt