Construct: ORF TRCN0000474954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006889.1_s317c1
- Derived from:
- ccsbBroadEn_10811
- DNA Barcode:
- TCGCGCCCGGTTCGACTACGGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MECOM (2122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | NM_001366473.2 | 16.5% | 14.4% | (many diffs) |
| 2 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_017005877.1 | 16.5% | 14.3% | (many diffs) |
| 3 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247225.4 | 16.4% | 14.2% | (many diffs) |
| 4 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247224.3 | 16.3% | 14.2% | (many diffs) |
| 5 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | NM_001366466.2 | 12.2% | 10.6% | (many diffs) |
| 6 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247214.3 | 12.2% | 10.6% | (many diffs) |
| 7 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | NM_004991.4 | 12.1% | 10.5% | (many diffs) |
| 8 | human | 2122 | MECOM | MDS1 and EVI1 complex locus | XM_005247213.3 | 12.1% | 10.5% | (many diffs) |
| 9 | mouse | 14013 | Mecom | MDS1 and EVI1 complex locus | NM_021442.2 | 66.2% | 65.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag atccaaaggc agggcaagga aactggccac aaataatgag tgtgtatatg 121 gcaactaccc tgaaatacct ttggaagaaa tgccagatgc agatggagta gccagcactc 181 cctccctcaa tattcaagag ccatgctctc cTGCCACATC CAGTGAAGCA TTCACTCCAA 241 AGGAGGGTTC TCCTTACAAA GCCCCCATCT ACATCCCTGA TGATATCCCC ATTCCTGCTG 301 AGTTTGAACT TCGAGAGTCA AATATGCCTG GGGCAGGACT AGGAATATGG ACCAAAAGGA 361 AGATCGAAGT AGGTGAAAAG TTTGGGCCTT ATGTGGGAGA GCAGAGGTCA AACCTGAAAG 421 ACCCCAGTTA TGGATGGGAG GTACATCTTC CAAGGTCTCG GAGGGTAAGC GTTCACTCTT 481 GGTTGTATTT GGGGAAGAGA AGCTCAGACG TAGGAATAGC CTTCTCTCAG GCTGATGTCT 541 ACATGCCTGG ACTGCAGTGT GCCTTCCTCT CGTACCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 721 GCGCCCGGTT CGACTACGGG TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt