Construct: ORF TRCN0000474975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005330.1_s317c1
- Derived from:
- ccsbBroadEn_11392
- DNA Barcode:
- GGTCCTGAGCATTCGGAGTGCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENTPD4 (9583)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9583 | ENTPD4 | ectonucleoside triphosphate... | NM_001128930.2 | 91.3% | 85.2% | (many diffs) |
| 2 | human | 9583 | ENTPD4 | ectonucleoside triphosphate... | NM_004901.5 | 90.1% | 84.1% | (many diffs) |
| 3 | mouse | 100862375 | Entpd4b | ectonucleoside triphosphate... | XM_003688929.4 | 80.1% | 81% | (many diffs) |
| 4 | mouse | 67464 | Entpd4 | ectonucleoside triphosphate... | XM_006519453.2 | 80.1% | 81% | (many diffs) |
| 5 | mouse | 67464 | Entpd4 | ectonucleoside triphosphate... | NM_026174.3 | 79% | 80% | (many diffs) |
| 6 | mouse | 100862375 | Entpd4b | ectonucleoside triphosphate... | XM_003688928.3 | 79% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1779
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaggattggc atctcctgtc tttttcctgc ttcttggcat tttagcatat 121 ctccagtagg gtgtcctcga attctgaata ccaatttacg ccaaattatg gtcattagtg 181 tcctggctgc tgctgtttca cttttatatt tttctgttgt cataatccga aataagtatg 241 ggcgactaac cagagacaag aaatttcaaa ggtacctggc acgagttacc gacattgaag 301 ctacagacac caataacccc aatgtgaact atgggatcgt ggtggactgt ggtagcagtg 361 ggtctcgagt atttgtttac tgctggccaa ggcataatgg caatccacat gatctgttgg 421 atatcaggca aatgagggat aaaaaccgaa agccagtggt catgaagata aaaccgggca 481 tttcagaatt tgctacctct ccagagaaag tcagtgatta catttctcca cttttgaact 541 ttgctgcaga gcatgtgcca cgggcaaaac acaaagagac acctctctac attctctgca 601 cggctggaat gagaatcctc cccgaaagcc agcagaaagc tattctggaa gaccttctga 661 ccgatatccc cgtgcacttt gactttctgt tttctgactc tcatgcagaa gtaatttctg 721 ggaaacaaga aggtgtgtat gcttggattg gcattaattt tgtccttgga cgatttgagc 781 atattgaaga tgatgatgag gccgttgtgg aagttaacat tcctggaagt gaaagcagcg 841 aagccattgt ccgtaaaagg acagcgggca ttctcgacat gggcggcgtg tcgactcaga 901 tagcgtacga agtccccaaa actgaagaag tagctaaaaa cttgttagct gaatttaact 961 tgggatgtga tgttcaccaa actgagcatg tgtatcgagt ctatgtggcc acgtttcttg 1021 ggtttggtgg caatgctgct cgacagagat acgaagacag aatatttgcc aacaccattc 1081 aaaagaacag gctcctgggt aaacagactg gtctgactcc tgatatgccg tacttggacc 1141 cctgcctacc cctagacatt aaagatgaaa tccagcaaaa tggacaaacc atatacctac 1201 gagggactgg agactttgac ctGTGTCGAG AGACTATCCA GCCTTTCATG AATAAAACAA 1261 ACGAGACCCA GACTTCCCTC AATGGGGTCT ACCAGCCCCC AATTCACTTC CAGAACAGTG 1321 AATTCTATGG CTTCTCCGAA TTCTACTACT GCACCGAGGA TGTGTTACGA ATGGGGGGAG 1381 ACTACAATGC TGCTAAATTT ACTAAAGCTG CAAAGGATTA TTGTGCAACA AAGTGGTCCA 1441 TTTTGCGGGA ACGCTTTGAC CGAGGACTGT ACGCCTCTCA TGCTGACCTC CACAGGCTTA 1501 AGTATCAGTG CTTCAAATCG GCCTGGATGT TTGAGGTGTT TCATAGGGGC TTTTCGTTTC 1561 CTGTCAACTA TAAAAGCTTA AAGACTGCCT TGCAAGTTTA CGACAAGGAG GTTCAGTGGA 1621 CCCTTGGAGC CATCCTCTAC AGGACCCGCT TTCTACCATT AAGGAAAATA GGGATGCTGA 1681 TAATACCTTG CTCAGGGGGT TGTCCAAAGC TGTGTTGTGC AGTATGGCAG CCACCACCAT 1741 ATGGGGCCAC CAAACACTTG CAATGTGCTG CTTGGAATTG CCCAACTTTC TTGTACAAAG 1801 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1861 GAACTAGTCC GTAACTTGAA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 1921 GACGAGGTCC TGAGCATTCG GAGTGCCACA CGCGTTAAGT Cgacaatcaa cctctggatt 1981 acaaaatttg tgaaagatt