Construct: ORF TRCN0000474976

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008636.1_s317c1
Derived from:
ccsbBroadEn_05860
DNA Barcode:
TCCTTTCCATGGACTAAAGCAGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ASNS (440)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474976
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440 ASNS asparagine synthetase (glut... NM_001352496.2 99.9% 99.8% 629T>A
2 human 440 ASNS asparagine synthetase (glut... NM_001673.5 99.9% 99.8% 629T>A
3 human 440 ASNS asparagine synthetase (glut... NM_133436.3 99.9% 99.8% 629T>A
4 human 440 ASNS asparagine synthetase (glut... NM_183356.3 99.9% 99.8% 629T>A
5 human 440 ASNS asparagine synthetase (glut... NM_001178075.2 96.1% 96% 0_1ins63;566T>A
6 human 440 ASNS asparagine synthetase (glut... NM_001178076.2 85.1% 85% 0_1ins249;380T>A
7 human 440 ASNS asparagine synthetase (glut... NM_001178077.1 85.1% 85% 0_1ins249;380T>A
8 human 389652 ASNSP1 asparagine synthetase pseud... NR_146077.1 51.9% (many diffs)
9 human 107986827 CZ1P-ASNS CZ1P-ASNS readthrough NR_147989.1 47.6% (many diffs)
10 mouse 27053 Asns asparagine synthetase NM_012055.3 87.8% 94.1% (many diffs)
11 mouse 27053 Asns asparagine synthetase XM_006505092.3 87.8% 94.1% (many diffs)
12 mouse 27053 Asns asparagine synthetase XM_006505093.3 87.8% 94.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1749
ORF length:
1683
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg tggcatttgg gcgctgtttg gcagtgatga ttgcctttct gttcagtgtc
121tgagtgctat gaagattgca cacagaggtc cagatgcatt ccgttttgag aatgtcaatg
181gatacaccaa ctgctgcttt ggatttcacc ggttggcggt agttgacccg ctgtttggaa
241tgcagccaat tcgagtgaag aaatatccgt atttgtggct ctgttacaat ggtgaaatct
301acaaccataa gaagatgcaa cagcattttg aatttgaata ccagaccaaa gtggatggtg
361agataatcct tcatctttat gacaaaggag gaattgagca aacaatttgt atgttggatg
421gtgtgtttgc atttgtttta ctggatactg ccaataagaa agtgttcctg ggtagagata
481catatggagt cagacctttg tttaaagcaa tgacagaaga tggatttttg gctgtatgtt
541cagaagctaa aggtcttgtt acattgaagc actccgcgac tcccttttta aaagtggagc
601cttttcttcc tggacactat gaagttttgg atttaaagcc aaatggcaaa gttgcatccg
661tggaaatggt taaatatcat cactgtcggg atgaacccct gcacgccctc tatgacaatg
721tggagaaact ctttccaggt tttgagatag aaactgtgaa gaacaacctc aggatccttt
781ttaataatgc tgtaaagaaa cgtttgatga cagacagaag gattggctgc cttttatcag
841ggggcttgga ctccagcttg gttgctgcca ctctgttgaa gcagctgaaa gaagcccaag
901tacagtatcc tctccagaca tttgcaattg gcatggaaga cagccccgat ttactggctg
961ctagaaaggt ggcagatcat attggaagtg aacattatga agtccttttt aactctgagg
1021aaggcattca ggctctggat gaagtcatat tttccttgga aacttatgac attacaacag
1081ttcgtgcttc agtaggtatg tatttaattt ccaagtatat tcggaagaac acagatagcg
1141tggtgatctt ctctggagaa ggatcagatg aacttacgca gggttacata tattttcaca
1201aggctccttc tcctGAAAAA GCCGAGGAGG AGAGTGAGAG GCTTCTGAGG GAACTCTATT
1261TGTTTGATGT TCTCCGCGCA GATCGAACTA CTGCTGCCCA TGGTCTTGAA CTGAGAGTCC
1321CATTTCTAGA TCATCGATTT TCTTCCTATT ACTTGTCTCT GCCACCAGAA ATGAGAATTC
1381CAAAGAATGG GATAGAAAAA CATCTCCTGA GAGAGACGTT TGAGGATTCC AATCTGATAC
1441CCAAAGAGAT TCTCTGGCGA CCAAAAGAAG CCTTCAGTGA TGGAATAACT TCAGTTAAGA
1501ATTCCTGGTT TAAGATTTTA CAGGAATACG TTGAACATCA GGTTGATGAT GCAATGATGG
1561CAAATGCAGC CCAGAAATTT CCCTTCAATA CTCCTAAAAC CAAAGAAGGA TATTACTACC
1621GTCAAGTCTT TGAACGCCAT TACCCAGGCC GGGCTGACTG GCTGAGCCAT TACTGGATGC
1681CCAAGTGGAT CAATGCCACT GACCCTTCTG CCCGCACGCT GACCCACTAC AAGTCAGCTG
1741TCAAAGCTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1801CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1861TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCTTT CCATGGACTA AAGCAGGTAC
1921GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)