Construct: ORF TRCN0000474978
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015137.1_s317c1
- Derived from:
- ccsbBroadEn_06831
- DNA Barcode:
- TTAGGGTTTGTATCGAAATGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAC1 (5879)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474978
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5879 | RAC1 | Rac family small GTPase 1 | NM_006908.5 | 99.8% | 99.4% | 404C>T |
| 2 | human | 5879 | RAC1 | Rac family small GTPase 1 | NM_018890.4 | 90.8% | 90.5% | 226_282del;461C>T |
| 3 | human | 5880 | RAC2 | Rac family small GTPase 2 | NM_002872.5 | 76.1% | 92.1% | (many diffs) |
| 4 | human | 5881 | RAC3 | Rac family small GTPase 3 | NM_005052.3 | 75.8% | 92.7% | (many diffs) |
| 5 | mouse | 19353 | Rac1 | RAS-related C3 botulinum su... | NM_009007.2 | 91.4% | 99.4% | (many diffs) |
| 6 | mouse | 19353 | Rac1 | RAS-related C3 botulinum su... | NM_001347530.1 | 83.2% | 90.5% | (many diffs) |
| 7 | mouse | 102631998 | Gm30181 | predicted gene, 30181 | XR_384352.1 | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggccatcaag tgtgtggtgg tgggagacGG AGCTGTAGGT AAAACTTGCC 121 TACTGATCAG TTACACAACC AATGCATTTC CTGGAGAATA TATCCCTACT GTCTTTGACA 181 ATTATTCTGC CAATGTTATG GTAGATGGAA AACCGGTGAA TCTGGGCTTA TGGGATACAG 241 CTGGACAAGA AGATTATGAC AGATTACGCC CCCTATCCTA TCCGCAAACA GATGTGTTCT 301 TAATTTGCTT TTCCCTTGTG AGTCCTGCAT CATTTGAAAA TGTCCGTGCA AAGTGGTATC 361 CTGAGGTGCG GCACCACTGT CCCAACACTC CCATCATCCT AGTGGGAACT AAACTTGATC 421 TTAGGGATGA TAAAGACACG ATCGAGAAAC TGAAGGAGAA GAAGCTGATT CCCATCACCT 481 ATCCGCAGGG TCTAGCCATG GCTAAGGAGA TTGGTGCTGT AAAATACCTG GAGTGCTCGG 541 CGCTCACACA GCGAGGCCTC AAGACAGTGT TTGACGAAGC GATCCGAGCA GTCCTCTGCC 601 CGCCTCCCGT GAAGAAGAGG AAGAGAAAAT GCCTGCTGTT GTACCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGATTA GGGTTTGTAT CGAAATGACC CACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t