Construct: ORF TRCN0000475008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007002.1_s317c1
- Derived from:
- ccsbBroadEn_05108
- DNA Barcode:
- CGCAAACGCTTCGTTCCAGATGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPTC7 (160760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475008
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 160760 | PPTC7 | PTC7 protein phosphatase ho... | NM_139283.2 | 100% | 100% | |
| 2 | human | 160760 | PPTC7 | PTC7 protein phosphatase ho... | XM_024448869.1 | 83.5% | 81.2% | 724_725ins130;762_763insTAGTGGCTGAGTATACAGAC |
| 3 | human | 160760 | PPTC7 | PTC7 protein phosphatase ho... | XM_024448870.1 | 81.8% | 80.5% | (many diffs) |
| 4 | human | 160760 | PPTC7 | PTC7 protein phosphatase ho... | NR_138550.2 | 14.2% | 1_287del;689_690ins199;1001_4795del | |
| 5 | mouse | 320717 | Pptc7 | PTC7 protein phosphatase ho... | NM_177242.4 | 87.6% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 981
- ORF length:
- 912
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttctcggtc ctctcgtacg ggcggctggt ggcccgcgcc gtgctcggcg 121 gcctctcgca gaccgacccc agggccggcg gcggcggcgg cggcgactac ggactggtga 181 cggccggctg cggcttcggg aaggacttcc gtaagggcct cctcaagaag ggcgcgtgct 241 acggggacga cgcgtgcttc gtggcccggc accgttccgc ggacgtgctc ggggttgcag 301 atggtgtagg aggctggaga gactatggag ttgatccatc tcaattctca gggactttaa 361 tgcggacgtg tgaacgttta gtaaaagaag gacggttcgt acctagtaat cccattggaa 421 ttctcaccac aagctactgt gagttgctgc aaaataaagt ccctttgctc ggtagcagca 481 ccgcctgcat tgtggtgctg gacagaaCCA GCCACCGCTT ACACACAGCA AACCTGGGCG 541 ATTCAGGCTT CCTGGTTGTC AGGGGTGGTG AAGTCGTGCA CCGATCAGAT GAGCAGCAGC 601 ATTACTTCAA CACTCCATTC CAGCTCTCAA TCGCTCCCCC TGAAGCCGAG GGAGTCGTCT 661 TGAGCGACAG TCCGGATGCT GCTGATAGCA CGTCTTTCGA TGTCCAGCTA GGAGACATTA 721 TCCTGACGGC AACAGATGGA CTCTTTGACA ACATGCCTGA TTATATGATT CTTCAGGAGC 781 TAAAAAAGTT AAAGAATTCA AATTATGAGA GTATACAACA GACTGCCAGA AGCATTGCTG 841 AGCAAGCTCA TGAGCTGGCC TATGACCCAA ATTATATGTC ACCTTTTGCA CAGTTTGCAT 901 GTGACAATGG ATTGAATGTG AGAGGTGGAA AGCCAGATGA CATCACCGTC CTTCTTTCAA 961 TAGTGGCTGA GTATACAGAC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGCA AACGCTTCGT 1141 TCCAGATGTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt