Construct: ORF TRCN0000475010

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007695.1_s317c1
Derived from:
ccsbBroadEn_09288
DNA Barcode:
TCTTTAGGCCTCTTCACTATATCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HS6ST2 (90161)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475010
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... NM_147175.4 99.9% 100% 9G>T
2 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... XM_005262491.3 98.1% 98.2% 9G>T;946_978del
3 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... NM_001077188.2 93.7% 93.7% 9G>T;948_1067del
4 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... XM_017029946.1 74.5% 74.5% 0_1ins438;508_540del
5 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... XM_011531406.1 71.1% 71.1% 0_1ins438;510_629del
6 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... XM_017029945.1 71.1% 71.1% 0_1ins438;510_629del
7 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... XM_011531407.3 54.5% 52.1% (many diffs)
8 human 90161 HS6ST2 heparan sulfate 6-O-sulfotr... XM_011531408.3 53.7% 52.2% (many diffs)
9 mouse 50786 Hs6st2 heparan sulfate 6-O-sulfotr... NM_001290467.1 90.5% 91.5% (many diffs)
10 mouse 50786 Hs6st2 heparan sulfate 6-O-sulfotr... XM_006541516.3 86.4% 87.2% (many diffs)
11 mouse 50786 Hs6st2 heparan sulfate 6-O-sulfotr... NM_001077202.2 84.9% 85.8% (many diffs)
12 mouse 50786 Hs6st2 heparan sulfate 6-O-sulfotr... NM_015819.4 68.7% 71.2% (many diffs)
13 mouse 50786 Hs6st2 heparan sulfate 6-O-sulfotr... NM_001290468.1 64.5% 66.8% (many diffs)
14 mouse 50786 Hs6st2 heparan sulfate 6-O-sulfotr... XM_006541518.2 64.5% 66.8% (many diffs)
15 mouse 50786 Hs6st2 heparan sulfate 6-O-sulfotr... XM_006541519.3 64.5% 66.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1884
ORF length:
1815
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcacttcct gcgtgtgcag tccgggagtt cgagccgccg cggcaaccgg
121agcgaggagc gcccgtccgc accacctgtc cccgccggca ttccagagta gaggccgaat
181tggcagcgag ccggcccggg tcggtcgccg cctcagttcg cgcgggccct cctaggggtg
241tgtctcacgg attccacacc cggccgctcc tggacaagcc ccgaaaggcg tcttcttccc
301tggcgggagc cgcgtgcgcc ccgcttttcg cgctgctgtc ccggggccgc cgcaggcgga
361tgcacgtcct caggcgacgc tgggacctgg gctccctctg ccgggccctg ctcactcggg
421gcctggccgc cctgggccac tcgctgaagc acgtgctcgg tgcgatcttc tccaagattt
481tcggccccat ggccagcgtc gggaacatgg atgagaaatc caacaagctg ctgctagctt
541tggtgatgct cttcctattt gccgtgatcg tcctccaata cgtgtgcccc ggcacagaat
601gccagctcct ccgcctgcag gcgttcagct ccccggtgcc ggacccgtac cgctcggagg
661atgagagctc cgccaggttc gtgccccgct acaatttcac ccgcggcgac ctcctgcgca
721aggtagactt cgacatcaag ggcgatgacc tgatcgtgtt cctgcacatc cagaagaccg
781ggggcaccac tttcggccgc cacttggtgc gtaacatcca gctggagcag ccgtgcgagt
841gccgcgtggg tcagaagaaa tgcacttgcc accggccggg taagcgggaa acctggctct
901tctccaggtt ctccacgggc tggagctgcg ggttgcacgc cgactggacc gagctcacca
961gctgtgtgcc ctccgtggtg gacggcaagc gcgacgccag gctgagaccg tccaggaact
1021tccactacat caccatcctc cgagacccag tgtcccggta cttgagtgag tggaggcatg
1081tccagagagg ggcaacatgg aaagcatccc tgcatgtctg cgatggaagg cctccaacct
1141ccgaagagct gcccagctgc tacactggcg atgactggtc tggctgcccc ctcaaagagt
1201ttatggactg tccctacaat ctagccaaca accgccaggt gcgcatgctc tccgacctga
1261ccctggtagg ctgctacaac ctctctgtca tgcctgaaaa gcaaagaaac aaggtccttc
1321tggaaagtgc caagtcaaat ctgaagcaca tggcgttctt cggcctcact gagtttcagc
1381ggaagaccca atatctgttt gagaaaacct tcaacatgaa ctttatttcg ccatttaccc
1441agtataatac cactagggcc tctagtgtag agatcaatga ggaaattcaa aagcgtattg
1501agggactgaa ttttctggat atggagttgt acagctatgc caaagacctt tttttGCAGA
1561GGTATCAGTT TATGAGGCAG AAAGAGCATC AGGAGGCCAG GCGAAAGCGT CAGGAACAAC
1621GCAAATTTCT GAAGGGAAGG CTCCTTCAGA CCCATTTCCA GAGCCAGGGT CAGGGCCAGA
1681GCCAGAATCC GAATCAGAAT CAGAGTCAGA ACCCAAATCC GAATGCCAAT CAGAACCTGA
1741CTCAGAATCT GATGCAGAAT CTGACTCAGA GTTTGAGCCA GAAGGAGAAC CGGGAAAGCC
1801CGAAGCAGAA CTCAGGCAAG GAGCAGAATG ATAACACCAG CAATGGCACC AACGACTACA
1861TAGGCAGTGT AGAGAAATGG CGTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1921AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1981TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CTTTAGGCCT
2041CTTCACTATA TCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2101att

Download FASTA (ORF) (Full)