Transcript: Mouse XM_006541518.2

PREDICTED: Mus musculus heparan sulfate 6-O-sulfotransferase 2 (Hs6st2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Hs6st2 (50786)
Length:
3898
CDS:
115..1635

Additional Resources:

NCBI RefSeq record:
XM_006541518.2
NBCI Gene record:
Hs6st2 (50786)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006541518.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000103147 CGCCGTTTACCCAGTATAATA pLKO.1 1157 CDS 100% 15.000 21.000 N Hs6st2 n/a
2 TRCN0000313624 TCGCCGTTTACCCAGTATAAT pLKO_005 1156 CDS 100% 15.000 21.000 N Hs6st2 n/a
3 TRCN0000103146 GCTCTTCCTATTTGCGGTGAT pLKO.1 156 CDS 100% 4.050 5.670 N Hs6st2 n/a
4 TRCN0000317181 GCTCTTCCTATTTGCGGTGAT pLKO_005 156 CDS 100% 4.050 5.670 N Hs6st2 n/a
5 TRCN0000313577 GCGTCAGGAGCAACGCAAATT pLKO_005 1335 CDS 100% 13.200 9.240 N Hs6st2 n/a
6 TRCN0000103145 CGTGTATCTTACAAGATGAAA pLKO.1 1690 3UTR 100% 5.625 3.938 N Hs6st2 n/a
7 TRCN0000317120 CGTGTATCTTACAAGATGAAA pLKO_005 1690 3UTR 100% 5.625 3.938 N Hs6st2 n/a
8 TRCN0000036301 GCTTTGGTGATGCTCTTCCTA pLKO.1 145 CDS 100% 3.000 2.100 N HS6ST2 n/a
9 TRCN0000103149 CCCAAATCAGAACCTGACTCA pLKO.1 1458 CDS 100% 2.640 1.848 N Hs6st2 n/a
10 TRCN0000103148 GCTAAAGACCTCTTTCTGCAA pLKO.1 1267 CDS 100% 2.640 1.848 N Hs6st2 n/a
11 TRCN0000317182 GCTAAAGACCTCTTTCTGCAA pLKO_005 1267 CDS 100% 2.640 1.848 N Hs6st2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006541518.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09288 pDONR223 100% 64.5% 66.8% None (many diffs) n/a
2 ccsbBroad304_09288 pLX_304 0% 64.5% 66.8% V5 (many diffs) n/a
3 TRCN0000475010 TCTTTAGGCCTCTTCACTATATCT pLX_317 18.4% 64.5% 66.8% V5 (many diffs) n/a
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