Construct: ORF TRCN0000475028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016983.1_s317c1
- Derived from:
- ccsbBroadEn_07022
- DNA Barcode:
- GTTAGAAAACAATAGATGGTGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAMP1 (6843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475028
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6843 | VAMP1 | vesicle associated membrane... | NM_199245.3 | 99.7% | 100% | 252C>A |
| 2 | human | 6843 | VAMP1 | vesicle associated membrane... | NM_001297438.2 | 98% | 96.5% | (many diffs) |
| 3 | human | 6843 | VAMP1 | vesicle associated membrane... | NM_016830.4 | 96% | 96.5% | 252C>A;341_342insTAAGTATC;344_348delGGGAC |
| 4 | human | 6843 | VAMP1 | vesicle associated membrane... | NM_014231.5 | 95.2% | 95.7% | (many diffs) |
| 5 | human | 6843 | VAMP1 | vesicle associated membrane... | NR_123717.2 | 28.6% | (many diffs) | |
| 6 | mouse | 22317 | Vamp1 | vesicle-associated membrane... | NM_001080557.1 | 93.1% | 98.2% | (many diffs) |
| 7 | mouse | 22317 | Vamp1 | vesicle-associated membrane... | NM_009496.3 | 88.8% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgctccagcT CAGCCACCTG CTGAAGGGAC AGAAGGGACT GCCCCAGGTG 121 GGGGTCCCCC TGGCCCTCCT CCTAACATGA CCAGTAACAG ACGACTACAG CAAACCCAGG 181 CACAAGTGGA GGAGGTGGTG GACATCATAC GTGTGAACGT GGACAAGGTC CTGGAGAGGG 241 ACCAGAAGCT GTCAGAGCTG GATGACCGAG CTGATGCCTT GCAGGCAGGA GCATCACAAT 301 TTGAGAGCAG TGCTGCAAAG CTAAAGAGGA AGTATTGGTG GAAAAACTGC AAGATGATGA 361 TCATGCTGGG AGCCATCTGT GCCATCATCG TGGTAGTTAT TGTAAGTAAG TATCGCTGCC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GAGTTAGAAA ACAATAGATG GTGTGAACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt