Construct: ORF TRCN0000475053

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018904.2_s317c1
Derived from:
ccsbBroadEn_14822
DNA Barcode:
AGCACTCTCGGTAAAAATCCACGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ALDH18A1 (5832)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475053
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323413.2 99% 29.8% (many diffs)
2 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323414.2 99% 29.8% (many diffs)
3 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_002860.4 99% 29.8% (many diffs)
4 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001017423.2 98.8% 29.8% (many diffs)
5 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323415.2 98.8% 29.8% (many diffs)
6 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448094.1 94.9% 28.5% (many diffs)
7 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448095.1 94.9% 28.5% (many diffs)
8 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448096.1 94.8% 28.6% (many diffs)
9 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323417.2 94.6% 25.4% (many diffs)
10 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323412.2 85% 15.8% (many diffs)
11 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323416.2 85% 15.8% (many diffs)
12 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323418.2 84.9% 15.8% (many diffs)
13 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... XM_024448097.1 81.6% 15.1% (many diffs)
14 human 5832 ALDH18A1 aldehyde dehydrogenase 18 f... NM_001323419.2 72.4% 3.1% (many diffs)
15 mouse 56454 Aldh18a1 aldehyde dehydrogenase 18 f... NM_019698.2 87.1% 27.2% (many diffs)
16 mouse 56454 Aldh18a1 aldehyde dehydrogenase 18 f... NM_153554.2 86.9% 27.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
780
ORF length:
711
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gttgagtcaa gtttaccgct gtgggttcca gcccttcaac caacatcttc
121tgccctgggt caagtgtaca accgtcttca gatctcattg tatccagcct tcagtcatca
181gacatgttcg ttcttggagc aacatcccgt ttatcactgt acccctcagt cgtacacatg
241gcaagtcctt cgcccaccgc agtgagctga agcatgccaa gagaatcgtg gtgaagctcg
301gcagtgccgt ggtgacccga ggggatgaat gtggcctggc cctggggcgc ttggcatcta
361ttgttgagca ggtatcagtg ctgcagaatc agggcagaga gatgatgctg gtgaccagtg
421gagccgtagc ctttggcaaa caacgcttgc gccatgagat ccttctgtct cagagcgtgc
481ggcaggccct ccactcgggg cagaaccagc tgaaagaaat ggcaattcca gtcttagagg
541cacgagcctg tgcagctgcc ggacagagtg ggctgatggc cttgtatgag gctatgttta
601cccagtacag catctgtgct gcccagattt tggtgaccaa tttggatttc catgatgagc
661agaagcgccg gaacctcaat ggaacacttc atgaactcct tagaatgaac attgtcccca
721ttgtcaacac aaatgatgct gttgtccccc cagctgagcc caacagtgac ctgcaggggt
781aaatgttatt agtgttaaag ataatgatag cctgctgccc gactggctgt gaaatgaaac
841tgatctcttg atgtctttca gatgtagaag ccttttgaca gccccccagg ttcagatgat
901gcaaagctta ttgatatatt ttatcccgga gatcagcagt ctgtgacatt tggaaccaag
961tctagagtgg gaatgggtgg catggaagcc aaggtgaaag cagccctctg ggctttgcaa
1021ggtggcactt ctgttgttat tgccaatgga acccacccaa aggtgtctgg gcacgtcatc
1081acagacattg tggaggggaa gaaagttggt accttctttt cagaagtaaa gcctgcaggc
1141cctactgttg agcagcaggg agaaatggcg cgatctggag gaaggatgtt ggccaccttg
1201gaacctgagc agagagcaga aattatccat catctggctg atctgttgac ggaccagcgt
1261gatgagatcc tgttagccaa caaaaaagac ttggaggagg cagaggggag acttgcagct
1321cctctgctga aacgtttaag cctctccaca tccaaattga acagcctggc catcggtctg
1381cgacagatcg cagcctcctc ccaggacagc gtgggacgtg ttttgcgccg cacccgaatc
1441gccaaaaact tggaactgga acaagtgact gtcccaattg gagttctgct ggtgatcttt
1501gaatctcgtc ctgactgtct accccaggtg gcagctttgg ctatcgcaag tggcaatggc
1561ttgttactca aaggagggaa ggaggctgca cacagcaacc ggattctcca cctcctgacc
1621caggaggctc tctcaatcca tggagtcaag ggagcccgtg caactggtgg attccaagag
1681aagaagttga agatctttgc cgcttgacca aaatgataga tctgatcatt ccacgttgct
1741cttccccagc tgtccagaga catccagaaa gctgctaagg ggatcccagt gatggggcac
1801agcgaaggga tctgtcacat gtatgtggat tccgaggcca gtgttgataa ggtcaccagg
1861ctagtcagag actctaaatg tgaatatcca gctgcctgta atgctttgga gactttgtta
1921atccaccggg atctgctcag gacaccatta tttgaccaga tcattgatat gctgagagtg
1981gaacaggtaa aaattcatgc aggccccaaa tttgcctcct atctgacctt cagccccTCC
2041GAAGTGAAGT CACTCCGAAC TGAGTATGGG GACCTGGAAT TATGCATTGA AGTAGTGGAC
2101AACGTTCAGG ATGCCATTGA CCACATCCAC AAGTATGGCA GCTCCCACAC GGATGTCATC
2161GTCACAGAGG ACGAAAACAC AGCGGAGTTC TTCCTGCAGC ACGTAGACAG TGCCTGTGTG
2221TTCTGGAATG CCAGCACTCG CTTTTCTGAT GGTTACCGCT TTGGACTGGG AGCTGAAGTG
2281GGAATCAGTA CATCGAGAAT CCACGCCCGG GGACCAGTAG GACTTGAGGG ACTGCTTACT
2341ACTAAGTGGC TGCTGCGAGG GAAGGACCAC GTGGTCTCAG ATTTCTCAGA GCATGGAAGT
2401TTAAAATATC TTCATGAGAA CCTCCCTATT CCTCAGAGAA ACACCAACTT GCCAACTTTC
2461TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT
2521ACGTAGTAAT GAACTAGTCC GTAACTTGAA AAGTATTTCG ATTTCTTGGC TTTATATATC
2581TTGTGGAAAG GACGAAGCAC TCTCGGTAAA AATCCACGCA CGCGTTAAGT Cgacaatcaa
2641cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)

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