Construct: ORF TRCN0000475053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018904.2_s317c1
- Derived from:
- ccsbBroadEn_14822
- DNA Barcode:
- AGCACTCTCGGTAAAAATCCACGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ALDH18A1 (5832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475053
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323413.2 | 99% | 29.8% | (many diffs) |
| 2 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323414.2 | 99% | 29.8% | (many diffs) |
| 3 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_002860.4 | 99% | 29.8% | (many diffs) |
| 4 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001017423.2 | 98.8% | 29.8% | (many diffs) |
| 5 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323415.2 | 98.8% | 29.8% | (many diffs) |
| 6 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | XM_024448094.1 | 94.9% | 28.5% | (many diffs) |
| 7 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | XM_024448095.1 | 94.9% | 28.5% | (many diffs) |
| 8 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | XM_024448096.1 | 94.8% | 28.6% | (many diffs) |
| 9 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323417.2 | 94.6% | 25.4% | (many diffs) |
| 10 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323412.2 | 85% | 15.8% | (many diffs) |
| 11 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323416.2 | 85% | 15.8% | (many diffs) |
| 12 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323418.2 | 84.9% | 15.8% | (many diffs) |
| 13 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | XM_024448097.1 | 81.6% | 15.1% | (many diffs) |
| 14 | human | 5832 | ALDH18A1 | aldehyde dehydrogenase 18 f... | NM_001323419.2 | 72.4% | 3.1% | (many diffs) |
| 15 | mouse | 56454 | Aldh18a1 | aldehyde dehydrogenase 18 f... | NM_019698.2 | 87.1% | 27.2% | (many diffs) |
| 16 | mouse | 56454 | Aldh18a1 | aldehyde dehydrogenase 18 f... | NM_153554.2 | 86.9% | 27.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 780
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgagtcaa gtttaccgct gtgggttcca gcccttcaac caacatcttc 121 tgccctgggt caagtgtaca accgtcttca gatctcattg tatccagcct tcagtcatca 181 gacatgttcg ttcttggagc aacatcccgt ttatcactgt acccctcagt cgtacacatg 241 gcaagtcctt cgcccaccgc agtgagctga agcatgccaa gagaatcgtg gtgaagctcg 301 gcagtgccgt ggtgacccga ggggatgaat gtggcctggc cctggggcgc ttggcatcta 361 ttgttgagca ggtatcagtg ctgcagaatc agggcagaga gatgatgctg gtgaccagtg 421 gagccgtagc ctttggcaaa caacgcttgc gccatgagat ccttctgtct cagagcgtgc 481 ggcaggccct ccactcgggg cagaaccagc tgaaagaaat ggcaattcca gtcttagagg 541 cacgagcctg tgcagctgcc ggacagagtg ggctgatggc cttgtatgag gctatgttta 601 cccagtacag catctgtgct gcccagattt tggtgaccaa tttggatttc catgatgagc 661 agaagcgccg gaacctcaat ggaacacttc atgaactcct tagaatgaac attgtcccca 721 ttgtcaacac aaatgatgct gttgtccccc cagctgagcc caacagtgac ctgcaggggt 781 aaatgttatt agtgttaaag ataatgatag cctgctgccc gactggctgt gaaatgaaac 841 tgatctcttg atgtctttca gatgtagaag ccttttgaca gccccccagg ttcagatgat 901 gcaaagctta ttgatatatt ttatcccgga gatcagcagt ctgtgacatt tggaaccaag 961 tctagagtgg gaatgggtgg catggaagcc aaggtgaaag cagccctctg ggctttgcaa 1021 ggtggcactt ctgttgttat tgccaatgga acccacccaa aggtgtctgg gcacgtcatc 1081 acagacattg tggaggggaa gaaagttggt accttctttt cagaagtaaa gcctgcaggc 1141 cctactgttg agcagcaggg agaaatggcg cgatctggag gaaggatgtt ggccaccttg 1201 gaacctgagc agagagcaga aattatccat catctggctg atctgttgac ggaccagcgt 1261 gatgagatcc tgttagccaa caaaaaagac ttggaggagg cagaggggag acttgcagct 1321 cctctgctga aacgtttaag cctctccaca tccaaattga acagcctggc catcggtctg 1381 cgacagatcg cagcctcctc ccaggacagc gtgggacgtg ttttgcgccg cacccgaatc 1441 gccaaaaact tggaactgga acaagtgact gtcccaattg gagttctgct ggtgatcttt 1501 gaatctcgtc ctgactgtct accccaggtg gcagctttgg ctatcgcaag tggcaatggc 1561 ttgttactca aaggagggaa ggaggctgca cacagcaacc ggattctcca cctcctgacc 1621 caggaggctc tctcaatcca tggagtcaag ggagcccgtg caactggtgg attccaagag 1681 aagaagttga agatctttgc cgcttgacca aaatgataga tctgatcatt ccacgttgct 1741 cttccccagc tgtccagaga catccagaaa gctgctaagg ggatcccagt gatggggcac 1801 agcgaaggga tctgtcacat gtatgtggat tccgaggcca gtgttgataa ggtcaccagg 1861 ctagtcagag actctaaatg tgaatatcca gctgcctgta atgctttgga gactttgtta 1921 atccaccggg atctgctcag gacaccatta tttgaccaga tcattgatat gctgagagtg 1981 gaacaggtaa aaattcatgc aggccccaaa tttgcctcct atctgacctt cagccccTCC 2041 GAAGTGAAGT CACTCCGAAC TGAGTATGGG GACCTGGAAT TATGCATTGA AGTAGTGGAC 2101 AACGTTCAGG ATGCCATTGA CCACATCCAC AAGTATGGCA GCTCCCACAC GGATGTCATC 2161 GTCACAGAGG ACGAAAACAC AGCGGAGTTC TTCCTGCAGC ACGTAGACAG TGCCTGTGTG 2221 TTCTGGAATG CCAGCACTCG CTTTTCTGAT GGTTACCGCT TTGGACTGGG AGCTGAAGTG 2281 GGAATCAGTA CATCGAGAAT CCACGCCCGG GGACCAGTAG GACTTGAGGG ACTGCTTACT 2341 ACTAAGTGGC TGCTGCGAGG GAAGGACCAC GTGGTCTCAG ATTTCTCAGA GCATGGAAGT 2401 TTAAAATATC TTCATGAGAA CCTCCCTATT CCTCAGAGAA ACACCAACTT GCCAACTTTC 2461 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 2521 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AAGTATTTCG ATTTCTTGGC TTTATATATC 2581 TTGTGGAAAG GACGAAGCAC TCTCGGTAAA AATCCACGCA CGCGTTAAGT Cgacaatcaa 2641 cctctggatt acaaaatttg tgaaagatt