Construct: ORF TRCN0000475059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002635.1_s317c1
- Derived from:
- ccsbBroadEn_00401
- DNA Barcode:
- GCCACATACAAACGTAGACCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYB5A (1528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1528 | CYB5A | cytochrome b5 type A | NM_148923.4 | 100% | 100% | |
| 2 | human | 1528 | CYB5A | cytochrome b5 type A | NM_001190807.3 | 92.5% | 92.5% | 258_259ins30 |
| 3 | human | 1528 | CYB5A | cytochrome b5 type A | NM_001914.4 | 72.8% | 72.3% | 292C>A;294_295ins108 |
| 4 | human | 1528 | CYB5A | cytochrome b5 type A | XM_011525835.2 | 72.8% | 72.3% | 292C>A;294_295ins108 |
| 5 | mouse | 109672 | Cyb5a | cytochrome b5 type A (micro... | NM_025797.4 | 86.3% | 87.3% | (many diffs) |
| 6 | mouse | 109672 | Cyb5a | cytochrome b5 type A (micro... | NM_001348159.1 | 63.1% | 63.4% | (many diffs) |
| 7 | mouse | 109672 | Cyb5a | cytochrome b5 type A (micro... | XM_017317803.1 | 63.1% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagcagtcg gacgaggccg tgaagtacta caccctagag gagattcaga 121 agcacaacca cagcaagagc acctggctga TCCTGCACCA CAAGGTGTAC GATTTGACCA 181 AATTTCTGGA AGAGCATCCT GGTGGGGAAG AAGTTTTAAG GGAACAAGCT GGAGGTGACG 241 CTACTGAGAA CTTTGAGGAT GTCGGGCACT CTACAGATGC CAGGGAAATG TCCAAAACAT 301 TCATCATTGG GGAGCTCCAT CCAGATGACA GACCAAAGTT AAACAAGCCT CCGGAAACTC 361 TTATCACTAC TATTGATTCT AGTTCCAGTT GGTGGACCAA CTGGGTGATC CCTGCCATCT 421 CTGCAGTGGC CGTCGCCTTG ATGTATCGCC TATACATGGC AGAGGACTGC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAGCCACAT ACAAACGTAG ACCTTTAACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt