Construct: ORF TRCN0000475080
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006389.1_s317c1
- Derived from:
- ccsbBroadEn_04776
- DNA Barcode:
- AAAAACCCATATGCCCGTAGCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOPNL (123811)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475080
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NM_144600.4 | 100% | 100% | |
| 2 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NM_001304499.2 | 87.8% | 87.8% | 224_295del |
| 3 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NM_001304500.2 | 62% | 58.6% | 27_28ins198 |
| 4 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NM_001304498.2 | 59.7% | 59.7% | 312_313ins210 |
| 5 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NM_001304497.2 | 57.4% | 57.4% | 225_226ins222 |
| 6 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NM_001304502.2 | 52.5% | 52.5% | 224_295del;384_385ins210 |
| 7 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NR_130756.2 | 22% | 1_18del;242_341del;641_2364del | |
| 8 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NR_130755.2 | 19.3% | 1_18del;244_245ins85;456_2179del | |
| 9 | human | 123811 | FOPNL | FGFR1OP N-terminal like | NR_130757.2 | 10.6% | (many diffs) | |
| 10 | mouse | 66086 | Fopnl | Fgfr1op N-terminal like | XM_017317110.1 | 54.2% | 56.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 588
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gactgtggca gagttgaagg ctgttttaaa ggacaccttg gaaaaaaagg 121 gggtattagg gcatttaaaa gcaaggatcc gagctgaagt tttcaatgcc ctagatgatg 181 accgtgaacc ccgaccatca ttgtctcatg aaaaccTTCT AATTAATGAA TTAATTCGAG 241 AGTATTTAGA ATTCAACAAA TATAAGTATA CAGCATCTGT CCTCATAGCA GAATCTGGTC 301 AACCTGTAGT TCCGTTGGAC AGACAGTTTC TCATCCATGA ACTAAATGCA TTTGAAGAAT 361 CAAAGGATAA TACAATACCT CTTTTATATG GGATTTTAGC CCATTTCTTG CGTGGAACTA 421 AGGATGGCAT CCAGAATGCA TTTCTGAAAG GGCCTTCACT TCAGCCTTCA GACCCAAGTC 481 TTGGCAGACA ACCTAGTAGA AGAAAGCCAA TGGATGACCA CCTAAGAAAG GAGGAACAGA 541 AAAGTACTAA CATTGAAGAT CTTCATGTTT CTCAGGCAGT CAACAGATAC CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGAAAAAACC CATATGCCCG TAGCTCCACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt