Construct: ORF TRCN0000475084
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001964.1_s317c1
- Derived from:
- ccsbBroadEn_08130
- DNA Barcode:
- TGCTTGTGAGTACACCCCTGTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX8 (29886)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475084
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29886 | SNX8 | sorting nexin 8 | NM_013321.4 | 99.8% | 100% | 729A>G;1128T>C |
| 2 | human | 29886 | SNX8 | sorting nexin 8 | XM_011515329.2 | 96% | 93.2% | (many diffs) |
| 3 | human | 29886 | SNX8 | sorting nexin 8 | XM_011515330.2 | 95.5% | 94.2% | (many diffs) |
| 4 | human | 29886 | SNX8 | sorting nexin 8 | XM_017012083.1 | 88.4% | 88.6% | 0_1ins159;570A>G;969T>C |
| 5 | human | 29886 | SNX8 | sorting nexin 8 | XM_017012084.2 | 88.4% | 88.6% | 0_1ins159;570A>G;969T>C |
| 6 | mouse | 231834 | Snx8 | sorting nexin 8 | NM_172277.3 | 84.7% | 88.3% | (many diffs) |
| 7 | mouse | 231834 | Snx8 | sorting nexin 8 | XM_006504672.2 | 81.8% | 81% | (many diffs) |
| 8 | mouse | 231834 | Snx8 | sorting nexin 8 | XM_006504673.3 | 77% | 76.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tggccgcgcg atggacccgc tgcccgcggc tgcagtcggg gcggcagctg 121 aggcggaggc tgacgaggag gcggatcccc cggcgtcaga tctgccgaca ccccaggcca 181 tcgagcccca ggccatcgtg cagcaggtcc cagcccccag tcgaatgcag atgccgcagg 241 ggaacccgct gctgctgtcc cacaccctgc aggagctgct ggccagggac accgtgcagg 301 tggagctcat tccggagaag aagggcctct tcctgaagca tgtggagtat gaggtttcca 361 gccagcgctt caagtcctcg gtatacagac ggtacaatga cttcgtggtc ttccaggaga 421 tgctcctgca caagttcccc taccgtatgg tgcctgccct gccacccaag agaatgctgg 481 gagctgacag ggagttcatc gaggccagga ggagagccct gaagcgcttc gtcaacctgg 541 tggcgcgaca ccccctgttc tccgaggatg tggtcctcaa gctcttcctg tccttcagcg 601 gctcggatgt gcagaacaag ttaaaggagt cagcacagtg cgtcggggac gaattcctga 661 actgtaagct ggctaccagg gccaaggact tcctcccagc tgacatccag gctcagtttg 721 ccatcagccg ggagctgatc cggaacatct acaatagctt tcacaagctt cgcgacaggg 781 ccgagcggat cgcgtcgcgg gccatcgaca atgcggcaga tcttctcata ttcgggaagg 841 agctaagtgc aatagggtct gacacgaccc cgctgccctc ctgggccgct ctgaatagca 901 gcacgtgggg gtccctgaag caggctctga aaggcctgtc tgtggaattc gcgctgctcg 961 ccgacaaggc tgcacaacag ggtaagcagg aagagaacga cgtggtggag aagctgaacc 1021 tcttcttgga tctgctgcag tcctataagg acctgtgcga gcggcatgag aagggcgtgt 1081 tgcacaagca ccagcgggcc ctgcacaagt acagcctgat gaagaggcag atgatgagcg 1141 ccaccgcgca gaaccgcgag ccggagtccg tggagcagct GGAGTCCCGC ATCGTGGAGC 1201 AGGAGAACGC GATTCAGACG ATGGAGCTGC GGAACTACTT CTCCCTGTAC TGCCTGCACC 1261 AGGAGACGCA GCTCATCCAC GTCTACCTGC CCCTCACCTC CCACATCCTC CGCGCCTTCG 1321 TCAACTCTCA GATCCAAGGG CACAAGGAGA TGAGCAAGGT GTGGAACGAC CTGAGGCCCA 1381 AGCTCAGCTG CCTCTTTGCG GGACCACACA GCACCCTGAC CCCACCGTGC TCCCCGCCGG 1441 AGGACGGCCT GTGTCCTCAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1501 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGC CCGTAACTTG 1561 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCT TGTGAGTACA 1621 CCCCTGTATA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt