Construct: ORF TRCN0000475146
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015281.1_s317c1
- Derived from:
- ccsbBroadEn_06750
- DNA Barcode:
- ACAGAAAAAGGGAAGGGTGTATCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- POLR2H (5437)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475146
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_006232.4 | 100% | 100% | |
| 2 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278698.2 | 85.7% | 69.6% | 336_399del;515_525del |
| 3 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_006713666.3 | 85.7% | 69.6% | 336_399del;515_525del |
| 4 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_006713667.2 | 85.7% | 69.6% | 336_399del;515_525del |
| 5 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278714.1 | 81.3% | 80.6% | 251_252ins84 |
| 6 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_017006636.2 | 81.3% | 80.6% | 251_252ins84 |
| 7 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278699.2 | 76% | 76% | 0_1ins108 |
| 8 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278700.1 | 76% | 76% | 0_1ins108 |
| 9 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001346879.2 | 65.1% | 49.4% | 0_1ins108;228_291del;407_417del |
| 10 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_024453602.1 | 65.1% | 49.4% | 0_1ins108;228_291del;407_417del |
| 11 | human | 5437 | POLR2H | RNA polymerase II subunit H | NM_001278715.1 | 57.3% | 56.6% | 0_1ins108;143_144ins84 |
| 12 | human | 5437 | POLR2H | RNA polymerase II subunit H | XM_017006637.1 | 57.3% | 56.6% | 0_1ins108;143_144ins84 |
| 13 | mouse | 245841 | Polr2h | polymerase (RNA) II (DNA di... | NM_145632.2 | 92% | 100% | (many diffs) |
| 14 | mouse | 245841 | Polr2h | polymerase (RNA) II (DNA di... | XM_006522137.1 | 70.4% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgcgatggc gggcatcctg tttgaggata ttttcgatgt gaaggatatt gacccggagg 121 gcaagaagtt tgaccgagtg tctcgactgc attgtgagag tgaatctttc aagatggatc 181 taatcttaga tgtaaacatt caaatttacc ctgtagactt gggtgacaag tttcggttgg 241 tcatagctag taccttgtat gaagatggta ccctggatga tggtgaatac aaccccactg 301 atgataggcc ttccagggct gaccagtttg agtatgtaat gtatggaaaa gtgtacagga 361 ttgagggaga tgaaacttct actgaagcag caacacgcct cTCTGCGTAC GTGTCCTATG 421 GGGGCCTGCT CATGAGGCTG CAGGGGGATG CCAACAACCT GCATGGATTC GAGGTGGACT 481 CCAGAGTTTA TCTCCTGATG AAGAAGCTAG CCTTCTGAAC CTCGCCTGAA GCCAGCCTCT 541 CTGCCAAGTC ACTCAGGTCA TGGGCATTGT TCAAGCCTGA GTGGCAGCCG CTCTTGCTCA 601 CCTGTTGAGG AAGGGCTGGC TCACTGTCCA CCGTGGCGGC ATCTTTAACT GGCCTCCACT 661 CAATGGGAAA CTGACTCGCC TGTGAAAGAC ACAGTGGGAG AGCTGAAAAT GAATCAGAAG 721 CTTTATGTAT ATGATTTTTA AATTAAACTT TACTTTTTCA GACTGCCCCT AAAAAAAAAA 781 AAAAAAAACT CGAGGCATCT ATGTCGGGTG CGGAGAAAGA GGTAATGAAA TGGCACATGG 841 TCATAGCTGT TTCCTGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 901 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 961 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACAGAAAAA GGGAAGGGTG 1021 TATCCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt