Construct: ORF TRCN0000475190
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001133.1_s317c1
- Derived from:
- ccsbBroadEn_04048
- DNA Barcode:
- TGTCCCCTCAAAAGGAAGTCTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf16 (79095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475190
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79095 | C9orf16 | chromosome 9 open reading f... | NM_024112.4 | 100% | 100% | |
| 2 | human | 79095 | C9orf16 | chromosome 9 open reading f... | XM_011519004.1 | 62.5% | 60.9% | (many diffs) |
| 3 | mouse | 73737 | 1110008P14Rik | RIKEN cDNA 1110008P14 gene | NM_198001.2 | 90.3% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 318
- ORF length:
- 249
- Sequence:
-
1 TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA 61 AAGTTGGCAT GTCGGGCCCC AACGGAGACC TGGGGATGCC GGTGGAGGCG GGAGCGGAAG 121 GCGAGGAGGA CGGCTTCGGG GAAGCAGAAT ACGCTGCCAT CAACTCCATG CTGGACCAGA 181 TCAACTCCTG TCTGGACCAC CTGGAGGAGA AGAATGACCA CCTCCACGCC CGCCTCCAGG 241 AGCTGCTGGA GTCCAACCGG CAGACACGCC TGGAGTTCCA GCAGCAGCTC GGGGAGGCCC 301 CCAGTGATGC CAGCCCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 361 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 421 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTCCCC TCAAAAGGAA 481 GTCTGATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt