Construct: ORF TRCN0000475194
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007293.1_s317c1
- Derived from:
- ccsbBroadEn_13452
- DNA Barcode:
- CTACGGATGGCCGCCCGCTAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR27 (253769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475194
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253769 | WDR27 | WD repeat domain 27 | NM_001202550.1 | 99.9% | 99.7% | 1019G>A;1243A>T |
| 2 | human | 253769 | WDR27 | WD repeat domain 27 | NM_001350623.2 | 91.1% | 90.9% | 329_330ins192;827G>A;1051A>T |
| 3 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010669.2 | 91.1% | 90.9% | 329_330ins192;827G>A;1051A>T |
| 4 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010660.2 | 85.1% | 84.9% | 330_710del;1400G>A;1624A>T |
| 5 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535692.3 | 83.6% | 83% | (many diffs) |
| 6 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535687.3 | 83.4% | 82.7% | (many diffs) |
| 7 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535688.3 | 83.4% | 82.7% | (many diffs) |
| 8 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535689.3 | 83.4% | 82.7% | (many diffs) |
| 9 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535691.3 | 83.4% | 83% | (many diffs) |
| 10 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010662.2 | 83.4% | 83% | (many diffs) |
| 11 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535690.3 | 83.2% | 82% | (many diffs) |
| 12 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535685.3 | 82.9% | 82.3% | (many diffs) |
| 13 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010659.2 | 82.7% | 82.5% | (many diffs) |
| 14 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535684.3 | 82.6% | 80.8% | (many diffs) |
| 15 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535682.3 | 81.2% | 79.7% | (many diffs) |
| 16 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010658.2 | 81.2% | 79.7% | (many diffs) |
| 17 | human | 253769 | WDR27 | WD repeat domain 27 | NM_182552.5 | 80.9% | 78.9% | (many diffs) |
| 18 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535693.3 | 74.8% | 72.5% | (many diffs) |
| 19 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010663.2 | 72.6% | 70.9% | (many diffs) |
| 20 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010664.2 | 72.6% | 70.9% | (many diffs) |
| 21 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010665.2 | 72.6% | 70.9% | (many diffs) |
| 22 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010666.2 | 72.6% | 70.9% | (many diffs) |
| 23 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010667.2 | 71.9% | 71.5% | (many diffs) |
| 24 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010668.2 | 71.6% | 71.5% | (many diffs) |
| 25 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535694.3 | 71.6% | 71.4% | (many diffs) |
| 26 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010670.2 | 57.8% | 57.6% | (many diffs) |
| 27 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010671.2 | 57.8% | 57.6% | (many diffs) |
| 28 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010672.2 | 54.1% | 52.9% | (many diffs) |
| 29 | human | 253769 | WDR27 | WD repeat domain 27 | XM_017010673.2 | 53.4% | 52.8% | (many diffs) |
| 30 | human | 253769 | WDR27 | WD repeat domain 27 | NR_146875.1 | 52.9% | (many diffs) | |
| 31 | human | 253769 | WDR27 | WD repeat domain 27 | NM_001350624.1 | 46.7% | 44.1% | (many diffs) |
| 32 | human | 253769 | WDR27 | WD repeat domain 27 | NM_001350625.1 | 46.7% | 44.1% | (many diffs) |
| 33 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535696.3 | 43% | 40.6% | (many diffs) |
| 34 | human | 253769 | WDR27 | WD repeat domain 27 | XM_011535697.3 | 40.1% | 39.5% | (many diffs) |
| 35 | human | 253769 | WDR27 | WD repeat domain 27 | NR_146876.1 | 33.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2259
- ORF length:
- 2190
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaatccc caagacattt tctcaagtaa tggtggctgt ctaagtgata 121 tagttataga aaaatacctg gttgaatcca aggagtctgt gtctcatgtt cagcttgctt 181 gcagcatgca ggactgtgct ttccctttgg atggaactga actttgtata tggaacacta 241 aggatccttc tcatcagctt ctaatcctac gaggacacca tcagccaatt actgctatgg 301 cttttggaaa taaagtgaac ccacttctaa tctgctcagc atcactggat tatgttataa 361 tgtggaacct ggatgaatgt agagagaaag tacttcaagc atatcctctt ctcagtttat 421 tcattgatgc agaaagcagg cagctggtca ccgggtgtgc tgacggccag ctttggatct 481 tcagtttgat ggatggacac cattatcgtc gtgtggcacg ggttgaccta aggaagaaga 541 cagagacttt ctccacaaga agggttaagt ctgggctgtg cagccagcca gaagaaagcc 601 agcttccctc tacaagtgct ctgggaaaag gagagcaggt tgaagtaaca tttcctgtac 661 tgagacttgc accctgtgat ctctcactca tcccaaattc tgcatgtgga tgtctttctt 721 ctgagaacac ccgatgtgtg tggatcggaa gctcagtggg cttattcgta tttaacctgg 781 caaacctgga agtggaagct gctttgtatt acaaggattt ccagagcctc agcatcctgc 841 tggccggatc gtgtgccctg aggaaccgca ctgcggatca aaaggtgctg tgcttgctgg 901 cctccctctt tggcgggaag attgccgtgt tggagatcaa cccggccgcg ctagtcaggg 961 ctcagcagtg ccccagcatg gggcagagcc tctcggtgcc agccagctcc tgtgtcctac 1021 cgacctctcc actgtatctg ggaattgcca aggagaagag taccaaggct gctagtgaac 1081 agcgacatgc tgcacggaac gtcatgaagg accaacgcct ggttttccat agtaaagtta 1141 ggtcatctgg ttatgcgtca gcaccacatg taactatgtt ttcaccaaag accaacatca 1201 agagtgaggg gaaaggatct tcaaggagca ggagcagctg cgcacgggag gcataccccg 1261 tggagtgcgc tgtgcccacc aagcccggcc cccaggtcgc tgccgccccc tcctgcacac 1321 gtgtatgctg catccagtac tcaggagatg ggcagtggct ggcctgtggg ttggccaacc 1381 atctgttact cgtttttgat gccagcctga ctgggacacc tgctgtgttt tcaggtcacg 1441 acggggcagt gaatgccgtg tgctggagcc aggaccggag gtggctgctc tctgcggccc 1501 gggacgggac cctgcgaatg tggtcggctc gtggggcaga gctcgcactg cttctgggca 1561 aagacatgtt ttctaaacct atacagtctg cacagttcta ttatatagat gcctttatat 1621 tgttatcttc tggccctgaa tttcagctac tgaggtatca cattgacact tgcaaagatg 1681 agattaagag atataaacag aagagcaagt ccaagctgat ttgcaggctc tccacgacgg 1741 gtgcagtaga catgaccagt ttatcggcag tcaacgactt ttattcccac atcgtactcg 1801 cagctggccg gaacaggacc gtggaagtgt ttgacctcaa cgccggctgc agtgcagcgg 1861 tgatagcgga agcccactca cggcctgtcc atcaaatctg ccaaaataaa ggttcatcat 1921 ttacaaccca acagccTCAG GCTTATAACC TTTTCCTGAC CACGGCCATT GGCGATGGGA 1981 TGAGACTGTG GGACCTGAGA ACCCTGAGGT GTGAGCGCCA CTTTGAAGGG CATCCAACCC 2041 GCGGCTATCC ATGTGGAATC GCTTTCAGTC CTTGTGGACG ATTCGCGGCT TGTGGGGCCG 2101 AGGACAGACA CGCTTACGTG TATGAAATGG GCTCAAGCAC GTTTTCTCAC CGGCTGGCTG 2161 GGCACACAGA CACTGTCACT GGAGTGGCCT TCAACCCATC AGCGCCCCAG CTTGCCACAG 2221 CTACCTTGGA TGGTAAACTC CAGCTCTTTC TAGCTGAGTT GCCAACTTTC TTGTACAAAG 2281 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2341 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2401 ACGACTACGG ATGGCCGCCC GCTAGTGCAC GCGTTAAGTC gacaatcaac ctctggatta 2461 caaaatttgt gaaagatt