Transcript: Human NM_001350623.2

Homo sapiens WD repeat domain 27 (WDR27), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
WDR27 (253769)
Length:
2712
CDS:
487..2487

Additional Resources:

NCBI RefSeq record:
NM_001350623.2
NBCI Gene record:
WDR27 (253769)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001350623.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412866 CAGTCTGCACAGTTCTATTAT pLKO_005 1810 CDS 100% 15.000 21.000 N WDR27 n/a
2 TRCN0000431191 CCAACAGCCTCAGGCTTATAA pLKO_005 2154 CDS 100% 15.000 21.000 N WDR27 n/a
3 TRCN0000424520 AGCTACTGAGGTATCACATTG pLKO_005 1871 CDS 100% 10.800 15.120 N WDR27 n/a
4 TRCN0000431495 CACGCTTACGTGTATGAAATG pLKO_005 2335 CDS 100% 10.800 15.120 N WDR27 n/a
5 TRCN0000142507 GCTATCCATGTGGAATCGCTT pLKO.1 2270 CDS 100% 2.640 3.696 N WDR27 n/a
6 TRCN0000425437 AGTGGGCTTATTCGTATTTAA pLKO_005 981 CDS 100% 15.000 10.500 N WDR27 n/a
7 TRCN0000144221 CAGTGGGCTTATTCGTATTTA pLKO.1 980 CDS 100% 15.000 10.500 N WDR27 n/a
8 TRCN0000417919 GAGCAGGTTGAAGTAACATTT pLKO_005 859 CDS 100% 13.200 9.240 N WDR27 n/a
9 TRCN0000121951 CTGCTTTGTATTACAAGGATT pLKO.1 1025 CDS 100% 4.950 3.465 N WDR27 n/a
10 TRCN0000144265 CTGGATGAATGTAGAGAGAAA pLKO.1 787 CDS 100% 4.950 3.465 N WDR27 n/a
11 TRCN0000143202 GAATGCTGTGAGGAACAACTT pLKO.1 2513 3UTR 100% 4.950 3.465 N WDR27 n/a
12 TRCN0000142617 GTCCAGTACTTCTGCACAGAA pLKO.1 2569 3UTR 100% 4.950 3.465 N WDR27 n/a
13 TRCN0000141707 CGTGGAAGTGTTTGACCTCAA pLKO.1 2046 CDS 100% 4.050 2.835 N WDR27 n/a
14 TRCN0000145527 GCTGCTTTGTATTACAAGGAT pLKO.1 1024 CDS 100% 3.000 2.100 N WDR27 n/a
15 TRCN0000122494 CCTGTCCATCAAATCTGCCAA pLKO.1 2110 CDS 100% 2.640 1.848 N WDR27 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001350623.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13452 pDONR223 100% 91.1% 90.9% None 329_330ins192;827G>A;1051A>T n/a
2 ccsbBroad304_13452 pLX_304 0% 91.1% 90.9% V5 329_330ins192;827G>A;1051A>T n/a
3 TRCN0000475194 CTACGGATGGCCGCCCGCTAGTGC pLX_317 15% 91.1% 90.9% V5 329_330ins192;827G>A;1051A>T n/a
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