Construct: ORF TRCN0000475226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013472.1_s317c1
- Derived from:
- ccsbBroadEn_08238
- DNA Barcode:
- CCGGACCACAACGTAAAGCATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCTN4 (51164)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475226
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51164 | DCTN4 | dynactin subunit 4 | NM_016221.3 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 51164 | DCTN4 | dynactin subunit 4 | NM_001135643.1 | 98.2% | 98% | (many diffs) |
| 3 | human | 51164 | DCTN4 | dynactin subunit 4 | NM_001135644.1 | 87.3% | 87.3% | (many diffs) |
| 4 | human | 51164 | DCTN4 | dynactin subunit 4 | XM_011537644.1 | 87.3% | 87.3% | (many diffs) |
| 5 | human | 51164 | DCTN4 | dynactin subunit 4 | XM_011537645.1 | 87.3% | 87.3% | (many diffs) |
| 6 | human | 51164 | DCTN4 | dynactin subunit 4 | XM_011537646.1 | 82.6% | 82.6% | (many diffs) |
| 7 | human | 51164 | DCTN4 | dynactin subunit 4 | XM_005268457.1 | 68.7% | 68.9% | 0_1ins429;594C>A;948T>C |
| 8 | human | 51164 | DCTN4 | dynactin subunit 4 | XM_011537647.1 | 68.7% | 68.9% | 0_1ins429;594C>A;948T>C |
| 9 | mouse | 67665 | Dctn4 | dynactin 4 | NM_026302.3 | 89.4% | 96.3% | (many diffs) |
| 10 | mouse | 67665 | Dctn4 | dynactin 4 | XM_006526189.3 | 88% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1446
- ORF length:
- 1380
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtccttgctg cagtcggacc gggttctcta tctagtccag ggagaaaaga 121 aggttcgggc cccgctctcg caactctact tctgccgcta ttgtagcgaa ctgcggtcgc 181 tggaatgtgt gtctcacgag gtggnctccc attattgtcc cagttgttta gaaaatatgc 241 catcggctga agccaaacta aaaaagaata gatgtgccaa ttgttttgac tgtcctggct 301 gcatgcacac cctctctact cgggccacga gcatctccac acagcttaca gatgacccag 361 ccaagaccac catgaagaaa gcctattacc tggcatgtgg attttgtcgc tggacgtcta 421 gagatgtggg catggcagac aaatctgtag ctagtggcgg ttggcaggaa cctgaaaatc 481 ctcacacaca acggatgaac aaattgattg aatattacca gcagcttgct cagaaagaga 541 aggttgagcg agatcgcaag aaactggcac gacgtagaaa ctatatgcct ctggcttttt 601 cggacaaata tggtcttgga accaggcttc agcgaccacg agctggtgca tccatcagta 661 cccttgccgg actttccctt aaagaaggag aggatcagaa agagataaag attgagccag 721 ctcaggctgt ggatgaagtg gaacctctac ctgaagacta ttatacaaga ccagtaaatt 781 taacagaggt aacaaccctt cagcagcgtc tgttacagcc tgacttccag ccagtctgtg 841 cttcacagct ctatcctcgc cacaaacatc ttctgatcaa acggtccctg cgctgccgta 901 aatgtgaaca taatttgagc aagccagaat ttaacccaac gtcaatcaaa ttcaaaatcc 961 agctggtcgc tgtcaattat attccagaag tgagaatcat gtcaattccc aaccttcgct 1021 acatgaagga gagccaggtc cTCCTGACTC TTACAAATCC AGTTGAGAAC CTCACCCATG 1081 TGACTCTATT CGAGTGTGAG GAGGGGGACC CTGATGATAT CAACAGCACT GCTAAGGTGG 1141 TGGTGCCTCC CAAAGAGCTC GTTTTAGCTG GCAAGGATGC AGCAGCAGAG TACGATGAGT 1201 TGGCAGAACC TCAAGACTTT CAGGACGATC CTGACATTAT AGCCTTCAGA AAGGCCAACA 1261 AAGTGGGTAT TTTCATCAAA GTTACACCAC AGCGTGAGGA GGGTGAAGTG ACCGTGTGCT 1321 TCAAGATGAA GCATGATTTT AAAAACCTGG CAGCCCCCAT TCGCCCCATT GAAGAAAGTG 1381 ACCAGGGAAC AGAAGTCATC TGGCTCACCC AGCATGTGGA ACTTAGCTTG GGCCCACTTC 1441 TCCCTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1501 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1561 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCGGACCAC AACGTAAAGC ATTTAACGCG 1621 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt