Construct: ORF TRCN0000475250

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001678.1_s317c1
Derived from:
ccsbBroadEn_10690
DNA Barcode:
GAGGATAACAACCGCTTTCCTTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATP2B2 (491)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475250
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 491 ATP2B2 ATPase plasma membrane Ca2+... NM_001330611.3 60.7% 59.4% (many diffs)
2 human 491 ATP2B2 ATPase plasma membrane Ca2+... NM_001363862.1 60.4% 59.1% (many diffs)
3 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006489.2 60% 58.7% (many diffs)
4 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006488.2 59.7% 58.4% (many diffs)
5 human 491 ATP2B2 ATPase plasma membrane Ca2+... NM_001353564.1 58.5% 57.2% (many diffs)
6 human 491 ATP2B2 ATPase plasma membrane Ca2+... NM_001683.5 58.5% 57.2% (many diffs)
7 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006487.1 58.5% 57.2% (many diffs)
8 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006486.2 58.4% 57.2% (many diffs)
9 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006485.2 58.1% 56.9% (many diffs)
10 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006483.2 57.8% 56.6% (many diffs)
11 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006484.2 57.8% 56.6% (many diffs)
12 human 491 ATP2B2 ATPase plasma membrane Ca2+... NM_001001331.4 56.4% 55.2% (many diffs)
13 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_005265179.5 56.4% 55.2% (many diffs)
14 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_006713175.4 56.4% 55.2% (many diffs)
15 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_011533752.3 56.4% 55.2% (many diffs)
16 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006481.2 56.4% 55.2% (many diffs)
17 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006482.2 56.4% 55.2% (many diffs)
18 human 491 ATP2B2 ATPase plasma membrane Ca2+... XM_017006492.2 55.1% 53.9% (many diffs)
19 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_017321354.1 55.7% 58.3% (many diffs)
20 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241170.2 55.4% 58% (many diffs)
21 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241169.2 54.8% 57.3% (many diffs)
22 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... NM_001036684.3 53.7% 56.1% (many diffs)
23 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... NM_009723.6 53.7% 56.1% (many diffs)
24 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_017321353.1 53.7% 56.1% (many diffs)
25 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241168.2 53.6% 56.1% (many diffs)
26 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241167.2 53.4% 55.8% (many diffs)
27 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241166.2 53% 55.5% (many diffs)
28 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241165.2 52.3% 54.7% (many diffs)
29 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... NM_001347365.1 51.7% 54.1% (many diffs)
30 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241161.1 51.7% 54.1% (many diffs)
31 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241162.2 51.7% 54.1% (many diffs)
32 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XM_011241163.2 51.7% 54.1% (many diffs)
33 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XR_001785094.1 44.6% (many diffs)
34 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XR_868850.2 26.5% (many diffs)
35 mouse 11941 Atp2b2 ATPase, Ca++ transporting, ... XR_868849.2 21% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2235
ORF length:
2166
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggtgacatg accaacagcg acttttactc caaaaaccaa agaaatgagt
121cgagccatgg gggcgagttc gggtgcacaa tggaggagct ccgctccctc atggagctgc
181ggggcactga ggctgtggtc aagatcaagg agacttatgg ggacaccgaa gccatctgcc
241ggcgcctcaa aacctcacct gttgaaggtt tgccgggcac cgctccagac ctggaaaaga
301gaaagcaaat ttttgggcaa aactttatac ctccaaagaa gccaaaaacc ttcctgcagc
361tcgtgtggga ggcgctgcag gacgtgacgc tcatcatcct ggagattgcc gccatcatct
421ccctggggct gtccttctac cacccgcccg gcgagggcaa cgaaggatgt gcgacggccc
481agggtggggc agaggatgaa ggagaggcag aggcaggttg gatcgagggg gccgccattc
541tcctctcagt tatctgtgtg gtcctggtca cggccttcaa tgactggagc aaagagaaac
601agttccgggg cctgcagagc cgcatcgagc aggaacagaa atttaccgtg gtccgggctg
661gccaggtggt gcagatccct gtggctgaga tcgtggttgg ggacatagcc caggtcaaat
721atggtgacct cctccctgcc gacggcctct tcatccaggg caatgacctc aagattgatg
781aaagctccct aactggagag tctgaccagg tgcgcaagtc cgtggacaag gaccccatgc
841tgctgtcagg aacccacgtg atggagggct caggacggat gttggtgact gctgtgggtg
901tgaactctca gactggcatc atctttaccc tcctgggggc tggtggtgaa gaggaagaga
961agaaagacaa aaaagccaaa caacaggacg gggcagccgc catggagatg cagcccctca
1021agagtgccga gggcggcgac gctgacgaca ggaagaaggc cagcatgcac aagaaggaga
1081agtccgtgct gcagggcaag ctcaccaagc tggctgtgca gatcgggaag gcgggcttgg
1141tgatgtcagc catcacggtg atcatcctgg tgctctactt cactgtggac accttcgtgg
1201tcaacaagaa gccgtggctg cctgagtgca cgcccgtcta cgtgcagtac tttgtcaagt
1261tcttcatcat tggcgtgacg gtgctggtgg tcgccgtgcc cgaggggctc cctctggccg
1321tcaccatctc gttggcctat tcggtgaaga aaatgatgaa ggacaacaat ctggtacgcc
1381acctggatgc ctgtgagacc atgggcaatg ccacagccat ctgctcagac aagacaggca
1441cgctgaccac caatcgcatg acagtggtac aggcctatgt cggcgacgtc cactataaag
1501agatccccga ccccagctcc atcaacacca agaccatgga gctgctgatc aatgccatcg
1561ccatcaacag cgcctacacc accaagattc tgcccccaga gaaggagggc gccctgcctc
1621ggcaggtggg caacaagacg gagtgcggcc tgctgggctt cgtgctggac ctgaagcagg
1681actacgagcc cgtgcgcagc cagatgccag aggagaagtt gtacaaagtg tacaccttca
1741actccgtgcg caagtccatg agcactgtca tcaagctgcc cgacgagagc ttccgcatgt
1801acagcaaggg ggcttctgag atcgtgctca agaagtgctg caaaatcctc aatggggcgg
1861gagagcctcg tgtcttccgg ccccgcgacc gggacgagat ggtaaagaag gtgattgagc
1921ccatggcttg cgatgggctc cgcactatct gcgtggccta ccgcgacttc cccagcagcc
1981cggagccgga ctgggacaat gagaatgaca tcctcaacga actcacctGC ATCTGCGTGG
2041TGGGCATCGA GGACCCGGTG CGGCCAGAGG TCCCAGAAGC CATCCGCAAG TGCCAGCGGG
2101CAGGCATGTA TCCAGGAGAG GAACTGCATA ACCAAAGGGG TGACCAGCCC TCAGATGTGC
2161TTATTGGATT CCAGTACAAA CGCCACCAAA GCCAGCCCAC TGCTCTCCTA CAAGGAAGGA
2221AAGATCTGCA CGTGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2281CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAC TTGAAAGTAT
2341TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG AGGATAACAA CCGCTTTCCT
2401TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)