Construct: ORF TRCN0000475255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005067.1_s317c1
- Derived from:
- ccsbBroadEn_03586
- DNA Barcode:
- AATACCATTAGAACGTGCGCCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR33 (55339)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55339 | WDR33 | WD repeat domain 33 | NM_001006622.2 | 100% | 100% | |
| 2 | human | 55339 | WDR33 | WD repeat domain 33 | XM_017004435.1 | 72.7% | 66% | (many diffs) |
| 3 | human | 55339 | WDR33 | WD repeat domain 33 | NM_001006623.3 | 72.1% | 65.1% | (many diffs) |
| 4 | human | 55339 | WDR33 | WD repeat domain 33 | XM_017004436.2 | 71.8% | 65.1% | (many diffs) |
| 5 | human | 55339 | WDR33 | WD repeat domain 33 | XM_005263697.3 | 25.7% | 20.2% | (many diffs) |
| 6 | human | 55339 | WDR33 | WD repeat domain 33 | NM_018383.5 | 21.8% | 17.2% | (many diffs) |
| 7 | human | 55339 | WDR33 | WD repeat domain 33 | XM_011511436.1 | 21.8% | 17.2% | (many diffs) |
| 8 | mouse | 74320 | Wdr33 | WD repeat domain 33 | NM_001170966.1 | 89.2% | 88.9% | (many diffs) |
| 9 | mouse | 74320 | Wdr33 | WD repeat domain 33 | NM_001170970.1 | 67.4% | 67.4% | (many diffs) |
| 10 | mouse | 74320 | Wdr33 | WD repeat domain 33 | NM_001170967.1 | 66.6% | 59.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tacagaaatt ggttctcctc ctcgtttttt ccatatgcca aggttccagc 121 accaggcacc tcgacagctg ttttataagc gacctgattt tgcacaacag caagcaatgc 181 aacagcttac ttttgatgga aaacgaatga gaaaagctgt gaaccgaaaa accatagact 241 acaatccatc tgtaattaag tatttggaga acagaatatg gcaaagagac cagagagata 301 tgcgggcaat tcagcctgat gcaggttatt acaatgatct ggtcccacct ataggaatgt 361 tgaataatcc tatgaatgca gtaacaacaa aatttgttcg gacatcaaca aataaagtaa 421 agtgtcctgt atttgttgtt aggtggactc cagaaggaag acgcttggtc actggagctt 481 ctagtgggga gtttaccctg tggaatggac tcactttcaa ttttgaaaca atattacagg 541 ctcacgacag cccagtgagG GCCATGACGT GGTCACATAA TGACATGTGG ATGTTGACAG 601 CAGACCACGG AGGATATGTG AAATATTGGC AGTCGAACAT GAACAACGTC AAGATGTTCC 661 AGGCACATAA GGAGGCGATT AGAGAGGCCA GGTTTATACA CAATATACCA TTTTCTGTAG 721 TCCCTATTGT CATGGTTAAA TTATTCTCTA AGTGTATTCT GGGTGCAGAG ATGCATGGGC 781 TCTGTCAGTT TCTGGGAAAC TTTCTGCACC CTATAAACAC AATATTTTTC TTTGTTTTCA 841 CACATTCACC ATTTTGCTGG CACCTTTCTG AAGTAGTGTT GTCCCGGTAT CAGCCTTTGC 901 AATATGTTAG AGATGTACTG TCTGCCGCAT TTTGCACTGG TTTTCTCTTT TCATTTATGA 961 TTAATAATGT GTATACGTTA TTCCTTTTTA TTATCTACTG TGTAAGACAA GAATATTTCA 1021 TTCCAAATAA AGAATTCAGT CTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ATACCATTAG 1201 AACGTGCGCC AGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att