Construct: ORF TRCN0000475264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005875.1_s317c1
- Derived from:
- ccsbBroadEn_07971
- DNA Barcode:
- TTTCCAGAGACGCCTGCCATGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNKD (25953)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | NM_015488.5 | 99.7% | 99.2% | 34G>A;98C>T;139C>T |
| 2 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | XM_017003771.1 | 92.2% | 91.6% | (many diffs) |
| 3 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | NM_022572.4 | 86.8% | 78.9% | (many diffs) |
| 4 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | XM_017003772.1 | 79.4% | 71.7% | (many diffs) |
| 5 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | NM_001077399.3 | 31% | 20.4% | (many diffs) |
| 6 | mouse | 56695 | Pnkd | paroxysmal nonkinesiogenic ... | NM_001039509.1 | 90.3% | 95.3% | (many diffs) |
| 7 | mouse | 56695 | Pnkd | paroxysmal nonkinesiogenic ... | XM_006496167.3 | 83.6% | 86.1% | (many diffs) |
| 8 | mouse | 56695 | Pnkd | paroxysmal nonkinesiogenic ... | NM_019999.2 | 78.2% | 75% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggtggta gctgctacgg cgctgaagag ccggggggcg agaaatgccc 121 gcgtcctccg ggggattctc gcaggagcca cagctaacaa ggtttctcat aacaggaccc 181 gggccctgca aagccacagc tcctcagagg gcaaggagga acctgaaccc ctatccccgg 241 agctggaata cattcccaga aagaggggca agaaccccat gaaagctgtg ggactggcct 301 ggtacagcct gtacacccgc acctggctcg ggtacctctt ctaccgacag cagctgcgca 361 gggctcggaa tcgctaccct aaaggccact cgaaaaccca gccccgcctc ttcaatggag 421 tgaaggtgct tcccatccct gtcctctcgg acaactacag ctacctcatc atcgacaccc 481 aggcccagct ggctgtggct gtggaccctt cagaccctcg ggctgtgcag gcttccattg 541 aaaaggaagg ggtcaccttg gtcgccattc tgtgtactca caagcactgg gaccacagtg 601 gagggaaccg tgacctcagc cggcggcacc gggactgtcg ggtgtacggg agccctcagg 661 acggcatccc ctacctcacc catcccctgt gtcatcaaga tgtggtcagc gtgggacggc 721 ttcagatccg ggccctggct acacctggcc acacacaagg ccatctggtc tacctactgg 781 atggggagcc ctacaagggt ccctcctgcc tcttctcagg ggacctgctc ttcctctctg 841 gctgtgggcg gacctttgag ggcaatgcag agaccatgct gagctcactg gacactgtgc 901 tggggctagg ggatgacacc cttctgtggc ctggtcatga gtatgcagag gagaacctgg 961 gctttgcagg tgtggtggag cccgagaacc tggcccggga gaggaagatg cagtgggtgc 1021 agcggcagcg gctggagcgc aagggcacgt gcccatctac cctgggagag gagcgctcct 1081 acaacccgtt cctgagaacc cactgcctgg cgctacagga ggctcTGGGG CCGGGGCCGG 1141 GCCCCACTGG GGATGATGAC TACTCCCGGG CCCAGCTCCT GGAAGAGCTC CGCCGGCTGA 1201 AGGATATGCA CAAGAGCAAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTC CAGAGACGCC 1381 TGCCATGAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt