Construct: ORF TRCN0000475303
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016195.1_s317c1
- Derived from:
- ccsbBroadEn_10763
- DNA Barcode:
- TGCAACCTTACTCCTTTTATACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2C9 (1559)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475303
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1562 | CYP2C18 | cytochrome P450 family 2 su... | NM_001128925.2 | 33.7% | 31.5% | (many diffs) |
| 2 | human | 1559 | CYP2C9 | cytochrome P450 family 2 su... | NM_000771.4 | 32.9% | 32.6% | 482_489delCCTCACCCinsG;492T>G;494_1470del |
| 3 | human | 1557 | CYP2C19 | cytochrome P450 family 2 su... | NM_000769.4 | 31.7% | 30.2% | (many diffs) |
| 4 | human | 1562 | CYP2C18 | cytochrome P450 family 2 su... | NM_000772.3 | 29.7% | 27.7% | (many diffs) |
| 5 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_000770.3 | 28.9% | 25.5% | (many diffs) |
| 6 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198853.1 | 16.6% | 14.6% | (many diffs) |
| 7 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198855.1 | 16.6% | 14.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttctcttgtg gtccttgtgc tctgtctctc atgtttgctt ctcctttcac 121 tctggagaca gagctctggg agaggaaaac tccctcctgg ccccactcct ctcccagtga 181 ttggaaatat cctacagata ggtattaagg acatcagcaa aTCCTTAACC AATCTCTCAA 241 AGGTCTATGG CCCTGTGTTC ACTCTGTATT TTGGCCTGAA ACCCATAGTG GTGCTGCATG 301 GATATGAAGC AGTGAAGGAA GCCCTGATTG ATCTTGGAGA GGAGTTTTCT GGAAGAGGCA 361 TTTTCCCACT GGCTGAAAGA GCTAACAGAG GATTTGGAAT TGTTTTCAGC AATGGAAAGA 421 AATGGAAGGA GATCCGGCGT TTCTCCCTCA TGACGCTGCG GAATTTTGGG ATGGGGAAGA 481 GGAGCATTGA GGACCGTGTT CAAGAGGAAG CCCGCTGCCT TGTGGAGGAG TTGAGAAAAA 541 CCAAGGGTGG GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC AACCTTACTC CTTTTATACC 721 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t