Construct: ORF TRCN0000475329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003113.1_s317c1
- Derived from:
- ccsbBroadEn_04583
- DNA Barcode:
- TCGAATGCTGCACACATTGCGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOB1B (92597)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92597 | MOB1B | MOB kinase activator 1B | NM_173468.4 | 100% | 100% | |
| 2 | human | 92597 | MOB1B | MOB kinase activator 1B | NM_001244766.2 | 97.2% | 94.6% | (many diffs) |
| 3 | human | 92597 | MOB1B | MOB kinase activator 1B | XM_005265709.2 | 97.2% | 94.6% | (many diffs) |
| 4 | human | 92597 | MOB1B | MOB kinase activator 1B | XM_011532412.2 | 87.3% | 87.9% | 1_81del;83_84delGAinsTG;86_87insCTTCTTGTT |
| 5 | human | 92597 | MOB1B | MOB kinase activator 1B | XM_017008837.1 | 86% | 85.4% | (many diffs) |
| 6 | human | 55233 | MOB1A | MOB kinase activator 1A | NM_018221.5 | 79.6% | 95.8% | (many diffs) |
| 7 | human | 55233 | MOB1A | MOB kinase activator 1A | NM_001317110.2 | 79.4% | 95.8% | (many diffs) |
| 8 | human | 55233 | MOB1A | MOB kinase activator 1A | XM_024452973.1 | 66.7% | 78.6% | (many diffs) |
| 9 | human | 92597 | MOB1B | MOB kinase activator 1B | NM_001244767.2 | 66.4% | 64.5% | (many diffs) |
| 10 | human | 55233 | MOB1A | MOB kinase activator 1A | NM_001317112.2 | 51% | 61.1% | (many diffs) |
| 11 | mouse | 68473 | Mob1b | MOB kinase activator 1B | NM_026735.2 | 91.8% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cttcttgttt ggtagtcgct cttctaaaac ttttaaacca aagaagaaca 121 ttccagaggg ttctcaccag tatgagctct taaaacacgc agaagccaca cttggcagtg 181 gcaaccttcg gatggctgtc atgcttcctg aaggggaaga tctcaatgaa tgggttgcag 241 ttaacactgt ggatTTCTTC AATCAGATCA ACATGCTTTA TGGAACTATC ACAGACTTCT 301 GTACAGAAGA GAGTTGTCCA GTGATGTCAG CTGGCCCAAA ATATGAGTAT CATTGGGCAG 361 ATGGAACGAA CATAAAGAAA CCTATTAAGT GCTCTGCACC AAAGTATATT GATTACTTGA 421 TGACTTGGGT TCAGGACCAG TTGGATGATG AGACGTTATT TCCATCAAAA ATTGGTGTCC 481 CGTTCCCAAA GAATTTCATG TCTGTGGCAA AAACTATACT CAAACGCCTC TTTAGGGTTT 541 ATGCTCACAT TTATCATCAG CATTTTGACC CTGTGATCCA GCTTCAGGAG GAAGCACATC 601 TAAATACATC TTTCAAGCAC TTTATTTTTT TTGTCCAGGA ATTCAACCTT ATTGATAGAA 661 GAGAACTTGC ACCACTCCAA GAACTGATTG AAAAACTCAC CTCAAAAGAC AGATACCCAA 721 CTTCCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAT CGAATGCTGC ACACATTGCG CCTACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att