Transcript: Human XM_011532412.2

PREDICTED: Homo sapiens MOB kinase activator 1B (MOB1B), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MOB1B (92597)
Length:
6966
CDS:
127..849

Additional Resources:

NCBI RefSeq record:
XM_011532412.2
NBCI Gene record:
MOB1B (92597)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011532412.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000312596 TTGGATGATGAGACGTTATTT pLKO_005 574 CDS 100% 15.000 21.000 N MOB1B n/a
2 TRCN0000052640 CTATACTCAAACGCCTCTTTA pLKO.1 647 CDS 100% 13.200 18.480 N MOB1B n/a
3 TRCN0000363565 CTATACTCAAACGCCTCTTTA pLKO_005 647 CDS 100% 13.200 18.480 N MOB1B n/a
4 TRCN0000052639 CCCGTTCCCAAAGAATTTCAT pLKO.1 612 CDS 100% 5.625 7.875 N MOB1B n/a
5 TRCN0000369234 AGGAGGAAGCACATCTAAATA pLKO_005 719 CDS 100% 15.000 10.500 N MOB1B n/a
6 TRCN0000312595 TACACTTGATAAGGGTAAATT pLKO_005 1046 3UTR 100% 15.000 10.500 N MOB1B n/a
7 TRCN0000369233 TGATTATGTGAAACCATATTC pLKO_005 993 3UTR 100% 13.200 9.240 N MOB1B n/a
8 TRCN0000052641 CAATCAGATCAACATGCTTTA pLKO.1 393 CDS 100% 10.800 7.560 N MOB1B n/a
9 TRCN0000327732 CAATCAGATCAACATGCTTTA pLKO_005 393 CDS 100% 10.800 7.560 N MOB1B n/a
10 TRCN0000052638 GCACTGTTTATTCCAGCTATA pLKO.1 4149 3UTR 100% 10.800 7.560 N MOB1B n/a
11 TRCN0000052642 CACTCCAAGAACTGATTGAAA pLKO.1 806 CDS 100% 5.625 3.938 N MOB1B n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4569 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4569 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011532412.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04583 pDONR223 100% 87.3% 87.9% None 1_81del;83_84delGAinsTG;86_87insCTTCTTGTT n/a
2 ccsbBroad304_04583 pLX_304 0% 87.3% 87.9% V5 1_81del;83_84delGAinsTG;86_87insCTTCTTGTT n/a
3 TRCN0000475329 TCGAATGCTGCACACATTGCGCCT pLX_317 70.3% 87.3% 87.9% V5 1_81del;83_84delGAinsTG;86_87insCTTCTTGTT n/a
4 ccsbBroadEn_14192 pDONR223 100% 71.2% 82.9% None (many diffs) n/a
5 ccsbBroad304_14192 pLX_304 0% 71.2% 82.9% V5 (not translated due to frame shift) (many diffs) n/a
6 TRCN0000492273 CTACTATATTAGCGCGTGCACTCC pLX_317 77% 71.2% 82.9% V5 (not translated due to frame shift) (many diffs) n/a
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