Construct: ORF TRCN0000475336

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000419.2_s317c1
Derived from:
ccsbBroadEn_12226
DNA Barcode:
ACGTACCCTCCGTCTCCCGTATAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NAGK (55577)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475336
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55577 NAGK N-acetylglucosamine kinase NM_017567.6 99.9% 100% 273G>C
2 human 55577 NAGK N-acetylglucosamine kinase NM_001330425.3 85% 85.1% 0_1ins153;120G>C
3 human 55577 NAGK N-acetylglucosamine kinase NM_001330426.2 85% 85.1% 0_1ins153;120G>C
4 human 55577 NAGK N-acetylglucosamine kinase NM_001365466.2 85% 85.1% 0_1ins153;120G>C
5 human 55577 NAGK N-acetylglucosamine kinase XM_011532957.1 85% 85.1% 0_1ins153;120G>C
6 human 55577 NAGK N-acetylglucosamine kinase XR_939694.1 59.9% (many diffs)
7 human 55577 NAGK N-acetylglucosamine kinase XM_017004440.1 55.5% 49.7% (many diffs)
8 mouse 56174 Nagk N-acetylglucosamine kinase NM_019542.2 86.9% 91.5% (many diffs)
9 mouse 56174 Nagk N-acetylglucosamine kinase NM_001347315.1 82.2% 84.8% (many diffs)
10 mouse 56174 Nagk N-acetylglucosamine kinase XM_006506408.2 77.8% 82.2% (many diffs)
11 mouse 56174 Nagk N-acetylglucosamine kinase NM_001164187.1 73.6% 78.1% (many diffs)
12 mouse 56174 Nagk N-acetylglucosamine kinase XM_006506409.2 63.5% 62.1% (many diffs)
13 mouse 56174 Nagk N-acetylglucosamine kinase XM_006506410.1 63.5% 62.1% (many diffs)
14 mouse 56174 Nagk N-acetylglucosamine kinase XM_017321685.1 63.5% 62.1% (many diffs)
15 mouse 56174 Nagk N-acetylglucosamine kinase XM_017321686.1 38.6% 36.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1098
ORF length:
1032
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cgcgatctat gggggtgtag aggggggagg cacacgatcc gaggtccttt
121tagtctcaga ggatgggaag atcctggcag aagcagatgg actgagcaca aaccactggc
181tgatcgggac agacaagtgt gtggagagga tcaatgagat ggtgaacagg gccaaacgga
241aagcaggggt ggatcctctg gtaccgctgc gaagcttggg cctatctctg agcggtgggg
301accaggagga cgcggggagg atcctgatcg aggagctcag ggaccgattt ccctacctga
361gtgaaagcta cttaatcacc accgatgccg ccggctccat cgccacagct acaccggatg
421gtggagttgt gctcatatct ggaacaggct ccaactgcag gctcatcaac cctgatggct
481ccgagagtgg ctgcggcggc tggggccata tgatgggtga tgagggttca gcctactgga
541tcgcacacca agcagtgaaa atagtgtttg actccattga caacctagag gcggctcctc
601atgatatcgg ctacgtcaaa caggccatgt tccactattt CCAGGTGCCA GATCGGCTAG
661GGATACTCAC TCACCTGTAT AGGGACTTTG ATAAATGCAG GTTTGCTGGG TTTTGCCGGA
721AAATTGCAGA AGGTGCTCAG CAGGGAGACC CCCTTTCCCG CTATATCTTC AGGAAGGCTG
781GGGAGATGCT GGGCAGACAC ATCGTAGCAG TGTTGCCCGA GATTGACCCG GTCTTGTTCC
841AGGGCAAGAT TGGACTCCCC ATCCTGTGCG TGGGCTCTGT GTGGAAGAGC TGGGAGCTGC
901TGAAGGAAGG TTTTCTTCTG GCGCTGACCC AGGGCAGAGA GATCCAGGCT CAGAACTTCT
961TCTCCAGCTT CACCCTGATG AAGCTGAGGC ACTCCTCCGC TCTGGGTGGG GCCAGCCTAG
1021GGGCCAGGCA CATCGGGCAC CTCCTCCCCA TGGACTATAG CGCCAATGCC ATTGCCTTCT
1081ATTCCTACAC CTTTTCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1141TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1201GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACGTACC CTCCGTCTCC
1261CGTATACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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