Transcript: Human XM_011532957.1

PREDICTED: Homo sapiens N-acetylglucosamine kinase (NAGK), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NAGK (55577)
Length:
1539
CDS:
525..1406

Additional Resources:

NCBI RefSeq record:
XM_011532957.1
NBCI Gene record:
NAGK (55577)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011532957.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000037619 CCTGAGTGAAAGCTACTTAAT pLKO.1 662 CDS 100% 13.200 18.480 N NAGK n/a
2 TRCN0000037621 CCCATGGACTATAGCGCCAAT pLKO.1 1353 CDS 100% 4.050 5.670 N NAGK n/a
3 TRCN0000322241 GTATAGGGACTTTGATAAATG pLKO_005 983 CDS 100% 13.200 9.240 N Nagk n/a
4 TRCN0000199925 GCAGATGGACTGAGCACAAAC pLKO.1 459 5UTR 100% 10.800 7.560 N NAGK n/a
5 TRCN0000381633 TAGCAGTGTTGCCCGAGATTG pLKO_005 1111 CDS 100% 10.800 7.560 N NAGK n/a
6 TRCN0000037620 ACTCACCTGTATAGGGACTTT pLKO.1 975 CDS 100% 4.950 3.465 N NAGK n/a
7 TRCN0000195309 CAGACACATCGTAGCAGTGTT pLKO.1 1100 CDS 100% 4.950 3.465 N NAGK n/a
8 TRCN0000199525 GATGGGAAGATCCTGGCAGAA pLKO.1 438 5UTR 100% 4.050 2.835 N NAGK n/a
9 TRCN0000037622 GCCTACTGGATCGCACACCAA pLKO.1 837 CDS 100% 0.880 0.616 N NAGK n/a
10 TRCN0000194814 CCAATGCCATTGCCTTCTATT pLKO.1 1369 CDS 100% 13.200 7.920 N NAGK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011532957.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12226 pDONR223 100% 85% 85.1% None 0_1ins153;120G>C n/a
2 ccsbBroad304_12226 pLX_304 0% 85% 85.1% V5 0_1ins153;120G>C n/a
3 TRCN0000475336 ACGTACCCTCCGTCTCCCGTATAC pLX_317 44.4% 85% 85.1% V5 0_1ins153;120G>C n/a
4 ccsbBroadEn_15103 pDONR223 0% 85% 85.1% None 0_1ins153;120G>C n/a
5 ccsbBroad304_15103 pLX_304 0% 85% 85.1% V5 0_1ins153;120G>C n/a
6 TRCN0000479754 TATCTCCTATGTTGTTCGCTGGGA pLX_317 35.3% 84.9% 84.8% V5 0_1ins153;120G>C;554G>T n/a
7 ccsbBroadEn_15902 pDONR223 0% 85% 84.8% None 0_1ins153;26C>T n/a
8 ccsbBroad304_15902 pLX_304 0% 85% 84.8% V5 0_1ins153;26C>T n/a
9 TRCN0000468607 TGTGGATTCCGACAATTGGGTTCG pLX_317 39.9% 84.9% 84.8% V5 0_1ins153;26C>T;873C>A n/a
10 TRCN0000489125 GTATTTGGCCCGATCACCTCAACA pLX_317 32.4% 85% 85.1% V5 (not translated due to prior stop codon) 0_1ins153;120G>C n/a
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