Transcript: Human NM_001330426.2

Homo sapiens N-acetylglucosamine kinase (NAGK), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
NAGK (55577)
Length:
2347
CDS:
166..1047

Additional Resources:

NCBI RefSeq record:
NM_001330426.2
NBCI Gene record:
NAGK (55577)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146503 TGCTTGGTGTGCGATCCAGT pXPR_003 AGG 319 36% 6 0.8543 NAGK NAGK 76324
2 BRDN0001148511 TTGACGTAGCCGATATCATG pXPR_003 AGG 384 44% 6 0.3253 NAGK NAGK 76323
3 BRDN0001146760 AAGCTTCGCAGCGGTACCAG pXPR_003 AGG 42 5% 3 -0.4012 NAGK NAGK 76325
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330426.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000037619 CCTGAGTGAAAGCTACTTAAT pLKO.1 303 CDS 100% 13.200 18.480 N NAGK n/a
2 TRCN0000037621 CCCATGGACTATAGCGCCAAT pLKO.1 994 CDS 100% 4.050 5.670 N NAGK n/a
3 TRCN0000322241 GTATAGGGACTTTGATAAATG pLKO_005 624 CDS 100% 13.200 9.240 N Nagk n/a
4 TRCN0000199925 GCAGATGGACTGAGCACAAAC pLKO.1 100 5UTR 100% 10.800 7.560 N NAGK n/a
5 TRCN0000381633 TAGCAGTGTTGCCCGAGATTG pLKO_005 752 CDS 100% 10.800 7.560 N NAGK n/a
6 TRCN0000037620 ACTCACCTGTATAGGGACTTT pLKO.1 616 CDS 100% 4.950 3.465 N NAGK n/a
7 TRCN0000195309 CAGACACATCGTAGCAGTGTT pLKO.1 741 CDS 100% 4.950 3.465 N NAGK n/a
8 TRCN0000199525 GATGGGAAGATCCTGGCAGAA pLKO.1 79 5UTR 100% 4.050 2.835 N NAGK n/a
9 TRCN0000037622 GCCTACTGGATCGCACACCAA pLKO.1 478 CDS 100% 0.880 0.616 N NAGK n/a
10 TRCN0000194814 CCAATGCCATTGCCTTCTATT pLKO.1 1010 CDS 100% 13.200 7.920 N NAGK n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2058 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000138140 GATTGAGACCATCCTGGCTAA pLKO.1 2113 3UTR 100% 4.050 2.025 Y LOC441087 n/a
13 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2059 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330426.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12226 pDONR223 100% 85% 85.1% None 0_1ins153;120G>C n/a
2 ccsbBroad304_12226 pLX_304 0% 85% 85.1% V5 0_1ins153;120G>C n/a
3 TRCN0000475336 ACGTACCCTCCGTCTCCCGTATAC pLX_317 44.4% 85% 85.1% V5 0_1ins153;120G>C n/a
4 ccsbBroadEn_15103 pDONR223 0% 85% 85.1% None 0_1ins153;120G>C n/a
5 ccsbBroad304_15103 pLX_304 0% 85% 85.1% V5 0_1ins153;120G>C n/a
6 TRCN0000479754 TATCTCCTATGTTGTTCGCTGGGA pLX_317 35.3% 84.9% 84.8% V5 0_1ins153;120G>C;554G>T n/a
7 ccsbBroadEn_15902 pDONR223 0% 85% 84.8% None 0_1ins153;26C>T n/a
8 ccsbBroad304_15902 pLX_304 0% 85% 84.8% V5 0_1ins153;26C>T n/a
9 TRCN0000468607 TGTGGATTCCGACAATTGGGTTCG pLX_317 39.9% 84.9% 84.8% V5 0_1ins153;26C>T;873C>A n/a
10 TRCN0000489125 GTATTTGGCCCGATCACCTCAACA pLX_317 32.4% 85% 85.1% V5 (not translated due to prior stop codon) 0_1ins153;120G>C n/a
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