Construct: ORF TRCN0000475351
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008819.1_s317c1
- Derived from:
- ccsbBroadEn_03110
- DNA Barcode:
- CCCCTATCGTTTAAACGGTCATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX12 (29934)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475351
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256185.1 | 100% | 100% | |
| 2 | human | 29934 | SNX12 | sorting nexin 12 | NM_013346.4 | 100% | 100% | |
| 3 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256188.1 | 97.5% | 97.5% | 152_153insGGTTCGCATGCG |
| 4 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256187.1 | 59.2% | 54.3% | 261_262ins125;288_289ins73 |
| 5 | human | 29934 | SNX12 | sorting nexin 12 | NM_001256186.1 | 56.7% | 51.8% | 152_153insGGTTCGCATGCG;249_250ins125;276_277ins73 |
| 6 | mouse | 55988 | Snx12 | sorting nexin 12 | NM_001110310.1 | 94.2% | 100% | (many diffs) |
| 7 | mouse | 55988 | Snx12 | sorting nexin 12 | NM_018875.2 | 90.8% | 96.3% | (many diffs) |
| 8 | mouse | 55988 | Snx12 | sorting nexin 12 | NM_001110311.1 | 89.2% | 94.1% | (many diffs) |
| 9 | mouse | 55988 | Snx12 | sorting nexin 12 | XM_011247646.2 | 73.7% | 78.2% | (many diffs) |
| 10 | mouse | 55988 | Snx12 | sorting nexin 12 | XM_011247645.2 | 70.5% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 555
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcggacacg gcagtagctg atacccggcg ccttaactcg aagccgcagg 121 acctgaccga cgcttacggg ccgccaagta acttcctgga gatcgacatc tttaatcctc 181 agacggtggg cgtgggacgc gcgcgcttca ccacctatga ggttcgcatg cggacaaacc 241 tacctatctt caagctaaag gagtcctgcg tacGGCGGCG CTACAGTGAC TTTGAGTGGC 301 TGAAAAATGA GCTGGAGAGA GATAGCAAGA TTGTAGTACC ACCACTGCCT GGGAAAGCCT 361 TGAAGCGGCA GCTCCCTTTC CGAGGAGATG AAGGGATCTT TGAGGAGTCT TTCATCGAAG 421 AAAGGAGGCA GGGCCTCGAG CAGTTTATTA ACAAAATTGC TGGGCACCCA CTGGCTCAGA 481 ATGAACGCTG CCTACACATG TTCCTGCAAG AGGAGGCAAT TGACAGGAAC TACGTCCCGG 541 GGAAGGTGCG CCAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCCCTATCGT TTAAACGGTC 721 ATTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt