Construct: ORF TRCN0000475354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000716.1_s317c1
- Derived from:
- ccsbBroadEn_13893
- DNA Barcode:
- TACTCCGAAAGATCATGAAACGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LTA4H (4048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475354
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4048 | LTA4H | leukotriene A4 hydrolase | NM_000895.3 | 99.9% | 1.9% | 12delA |
| 2 | human | 4048 | LTA4H | leukotriene A4 hydrolase | NM_001256643.1 | 93.5% | 2% | (many diffs) |
| 3 | human | 4048 | LTA4H | leukotriene A4 hydrolase | XM_005268871.2 | 87% | 2.2% | 12delA;1529_1530ins83;1596_1597ins154 |
| 4 | human | 4048 | LTA4H | leukotriene A4 hydrolase | XR_001748703.2 | 82.1% | (many diffs) | |
| 5 | human | 4048 | LTA4H | leukotriene A4 hydrolase | NM_001256644.1 | 80.7% | 2.3% | (many diffs) |
| 6 | human | 4048 | LTA4H | leukotriene A4 hydrolase | NR_132659.1 | 77.8% | (many diffs) | |
| 7 | human | 4048 | LTA4H | leukotriene A4 hydrolase | XM_011538349.3 | 76.8% | 2.5% | (many diffs) |
| 8 | human | 4048 | LTA4H | leukotriene A4 hydrolase | XR_002957326.1 | 62.5% | (many diffs) | |
| 9 | mouse | 16993 | Lta4h | leukotriene A4 hydrolase | NM_008517.2 | 87.2% | 1.9% | (many diffs) |
| 10 | mouse | 16993 | Lta4h | leukotriene A4 hydrolase | NM_001313897.1 | 75.7% | 2.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 168
- ORF length:
- 102
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgagatgtgg atacctgttc gttggcctct ccggcttccg tctgccggac 121 caagcacctg cacctgcgct gcagcgtcga ctttactcgc cggacgctga ccgggactgc 181 tgctctcacg gtccagtctc aggaggacaa tctgcgcagc ctggttttgg atacaaagga 241 ccttacaata gaaaaagtag tgatcaatgg acaagaagtc aaatatgctc ttggagaaag 301 acaaagttac aagggatcgc caatggaaat ctctcttcct atcgctttga gcaaaaatca 361 agaaattgtt atagaaattt cttttgagac ctctccaaaa tcttctgctc tccagtggct 421 cactcctgaa cagacttctg ggaaggaaca cccatatctc tttagtcagt gccaggccat 481 ccactgcaga gcaatccttc cttgtcagga cactccttct gtgaaattaa cctatactgc 541 agaggtgtct gtccctaaag aactggtggc acttatgagt gctattcgtg atggagaaac 601 acctgaccca gaagacccaa gcaggaaaat atacaaattc atccaaaaag ttccaatacc 661 ctgctacctg attgctttag ttgttggagc tttagaaagc aggcaaattg gcccaagaac 721 tttggtgtgg tctgagaaag agcaggtgga aaagtctgct tatgagtttt ctgagactga 781 atctatgctt aaaatagcag aagatctggg aggaccgtat gtatggggac agtatgacct 841 attggtcctg ccaccatcct tcccttatgg tggcatggag aatccttgcc ttacttttgt 901 aactcctact ctactggcag gcgacaagtc actctccaat gtcattgcac atgaaatatc 961 tcatagctgg acagggaatc tagtgaccaa caaaacttgg gatcactttt ggttaaatga 1021 gggacatact gtgtacttgg aacgccacat ttgcggacga ttgtttggtg aaaagttcag 1081 acattttaat gctctgggag gatggggaga actacagaat tcggtaaaga catttgggga 1141 gacacatcct ttcaccaaac ttgtggttga tctgacagat atagaccctg atgtagctta 1201 ttcttcagtt ccctatgaga agggctttgc tttacttttt taccttgaac aactgcttgg 1261 aggaccagag attttcctag gattcttaaa agcttatgtt gagaagtttt cctataagag 1321 cataactact gatgactgga aggatttcct gtattcctat tttaaagata aggttgatgt 1381 tctcaatcaa gttgattgga atgcctggct ctactctcct ggactgcctc ccataaagcc 1441 caattatgat atgactctga caaatgcttg tattgcctta agtcaaagat ggattactgC 1501 CAAAGAAGAT GATTTAAATT CATTCAATGC CACAGACCTG AAGGATCTCT CTTCTCATCA 1561 ATTGAATGAG TTTTTAGCAC AGACGCTCCA GAGGGCACCT CTTCCATTGG GGCACATAAA 1621 GCGAATGCAA GAGGTGTACA ACTTCAATGC CATTAACAAT TCTGAAATAC GATTCAGATG 1681 GCTGCGGCTC TGCATTCAAT CCAAGTGGGA GGACGCAATT CCTTTGGCGC TAAAGATGGC 1741 AACTGAACAA GGAAGAATGA AGTTTACCCG GCCCTTATTC AAGGATCTTG CTGCCTTTGA 1801 CAAATCCCAT GATCAAGCTG TCCGAACCTA CCAAGAGCAC AAAGCAAGCA TGCATCCCGT 1861 GACTGCAATG CTGGTGGGGA AAGACTTAAA AGTGGATAAC CCAACTTTCT TGTACAAAGT 1921 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1981 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 2041 CGATACTCCG AAAGATCATG AAACGCCACG CGTTAAGTCg acaatcaacc tctggattac 2101 aaaatttgtg aaagatt