Transcript: Human NM_001256643.1

Homo sapiens leukotriene A4 hydrolase (LTA4H), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
LTA4H (4048)
Length:
2145
CDS:
146..1909

Additional Resources:

NCBI RefSeq record:
NM_001256643.1
NBCI Gene record:
LTA4H (4048)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256643.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000050864 GCCTCCCATAAAGCCCAATTA pLKO.1 1435 CDS 100% 13.200 18.480 N LTA4H n/a
2 TRCN0000298974 GCCTCCCATAAAGCCCAATTA pLKO_005 1435 CDS 100% 13.200 18.480 N LTA4H n/a
3 TRCN0000050867 CGCAATTCCTTTGGCGCTAAA pLKO.1 1723 CDS 100% 10.800 15.120 N LTA4H n/a
4 TRCN0000331189 CGCAATTCCTTTGGCGCTAAA pLKO_005 1723 CDS 100% 10.800 15.120 N LTA4H n/a
5 TRCN0000050863 CGGCCCTTATTCAAGGATCTT pLKO.1 1778 CDS 100% 4.950 3.960 N LTA4H n/a
6 TRCN0000298973 CGGCCCTTATTCAAGGATCTT pLKO_005 1778 CDS 100% 4.950 3.960 N LTA4H n/a
7 TRCN0000050865 CCCTGCTACCTGATTGCTTTA pLKO.1 668 CDS 100% 10.800 7.560 N LTA4H n/a
8 TRCN0000298971 CCCTGCTACCTGATTGCTTTA pLKO_005 668 CDS 100% 10.800 7.560 N LTA4H n/a
9 TRCN0000050866 CCTTCTGTGAAATTAACCTAT pLKO.1 524 CDS 100% 4.950 3.465 N LTA4H n/a
10 TRCN0000298970 CCTTCTGTGAAATTAACCTAT pLKO_005 524 CDS 100% 4.950 3.465 N LTA4H n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256643.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13893 pDONR223 100% 93.5% 2% None (many diffs) n/a
2 ccsbBroad304_13893 pLX_304 0% 93.5% 2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000475354 TACTCCGAAAGATCATGAAACGCC pLX_317 22% 93.5% 2% V5 (not translated due to prior stop codon) (many diffs) n/a
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