Construct: ORF TRCN0000475396
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016313.1_s317c1
- Derived from:
- ccsbBroadEn_15536
- DNA Barcode:
- GAGTCATTGGTGCACCTCATGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRIM2 (5558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475396
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5558 | PRIM2 | DNA primase subunit 2 | NM_001282487.2 | 100% | 100% | |
| 2 | human | 5558 | PRIM2 | DNA primase subunit 2 | NM_001282488.1 | 100% | 100% | |
| 3 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_017011006.2 | 38.2% | 37.5% | (many diffs) |
| 4 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_017011005.2 | 32.1% | 31.4% | (many diffs) |
| 5 | human | 5558 | PRIM2 | DNA primase subunit 2 | NM_000947.5 | 30.8% | 30.2% | (many diffs) |
| 6 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_017011004.2 | 27.3% | 26.8% | (many diffs) |
| 7 | mouse | 19076 | Prim2 | DNA primase, p58 subunit | XM_006495797.2 | 55.1% | 58.2% | (many diffs) |
| 8 | mouse | 19076 | Prim2 | DNA primase, p58 subunit | XM_006495793.3 | 40.9% | 43.2% | (many diffs) |
| 9 | mouse | 19076 | Prim2 | DNA primase, p58 subunit | NM_008922.2 | 25.6% | 27.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gttttctgga agaaagtgga ggaagctgag gttggcaggt gaccagagga 121 atgcttccta ccctcattgc cttcagtttt acttgcagcc accTTCTGAA AACATATCTT 181 TAATAGAATT TGAAAACTTG GCTATTGATA GAGTTAAATT GTTAAAATCA GTTGAAAATC 241 TTGGAGTGAG CTATGTGAAA GGAACTGAAC AATACCAGAG TAAGTTGGAG AGTGAGCTTC 301 GGAAGCTCAA GTTTTCCTAC AGAGAAAACT TAGAAGATGA ATATGAACCA CGAAGAAGAG 361 ATCATATTTC TCATTTTATT TTGCGGCTTG CTTATTGCCA GTCTGAAGAA CTTAGACGCT 421 GGTTCATTCA ACAAGAAATG GATCTCCTTC GATTTAGATT TAGTATTTTA CCCAAGGATA 481 AAATTCAGGA TTTCTTAAAG GATAGCCAAT TGCAGTTTGA GGCTGTAAGT ATATTTTTGT 541 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGAGAGTC ATTGGTGCAC CTCATGTTCA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt