Construct: ORF TRCN0000475431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018837.1_s317c1
- Derived from:
- ccsbBroadEn_14530
- DNA Barcode:
- CGGATGAGTTATGACTAGGAGTGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ACVR2B (93)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93 | ACVR2B | activin A receptor type 2B | NM_001106.4 | 99.3% | 43.2% | (many diffs) |
| 2 | human | 93 | ACVR2B | activin A receptor type 2B | XM_017007515.2 | 96.7% | 39.6% | (many diffs) |
| 3 | human | 93 | ACVR2B | activin A receptor type 2B | XM_017007514.1 | 95.7% | 40.1% | (many diffs) |
| 4 | human | 93 | ACVR2B | activin A receptor type 2B | XM_017007516.1 | 95.4% | 40.1% | (many diffs) |
| 5 | human | 93 | ACVR2B | activin A receptor type 2B | XM_005265583.3 | 94.5% | 39.4% | (many diffs) |
| 6 | mouse | 11481 | Acvr2b | activin receptor IIB | NM_001313757.1 | 91.6% | 42.8% | (many diffs) |
| 7 | mouse | 11481 | Acvr2b | activin receptor IIB | XM_006511926.3 | 90% | 41.2% | (many diffs) |
| 8 | mouse | 11481 | Acvr2b | activin receptor IIB | NM_007397.3 | 87.5% | 40.9% | (many diffs) |
| 9 | mouse | 11481 | Acvr2b | activin receptor IIB | XR_379846.3 | 13.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 783
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggcgccc tgggtggccc tcgccctcct ctggggatcg ctgtgcgccg 121 gctctgggcg tggggaggct gagacacggg agtgcatcta ctacaacgcc aactgggagc 181 tggagcgcac caaccagagc ggcctggagc gctgcgaagg cgagcaggac aagcggctgc 241 actgctacgc ctcctggcgc aacagctctg gcaccatcga gctcgtgaag aagggctgct 301 ggctagatga cttcaactgc tacgataggc aggagtgtgt ggccactgag gagaaccccc 361 aggtgtactt ctgctgctgt gaaggcaact tctgcaacga gcgcttcact catttgccag 421 aggctggggg cccggaagtc acgtacgagc cacccccgac agcccccacc ctgctcacgg 481 tgctggccta ctcactgctg cccatcgggg gcctttccct catcgtcctg ctggcctttt 541 ggatgtaccg gcatcgcaag cccccctacg gtcatgtgga catccatgag gaccctgggc 601 ctccaccacc atcccctctg gtgggcctga agccactgca gctgctggag atcaaggctc 661 gggggcgctt tggctgtgtc tggaaggccc agctcatgaa tgactttgta gctgtcaaga 721 tcttcccact ccagacaagc agtcgtggca gagtgaacgg gagatcttca gcacacctgg 781 catgaagcac gagaacctgc tacagttcat tgctgccgag aagccgaggc tccaacctcg 841 aaagtagagc tgtggctcat cacggccttc catgacangg nnnccctcac ggattacntc 901 aaggggaaca tcatcacatg gaacgaactg tgtcatgtag cagagacgat gtcacgaggc 961 ctctcatacc tgcatgagga tgtgccctgg tgccgtggcg agggccacaa gccgtctatt 1021 gcccacaggg actttaaaag taagaatgta ttgctgaaga gcgacctcac agccgtgctg 1081 gctgactttg gcttggctgt tcgatttgag ccagggaaac ctccagggga cacccacgga 1141 caggtaggca cgagacggta catggctcct gaggtgctcg agggagccat caacttccag 1201 agagatgcct tcctgcGCAT TGACATGTAT GCCATGGGGT TGGTGCTGTG GGAGCTTGTG 1261 TCTCGCTGCA AGGCTGCAGA CGGACCCGTG GATGAGTACA TGCTGCCCTT TGAGGAAGAG 1321 ATTGGCCAGC ACCCTTCGTT GGAGGAGCTG CAGGAGGTGG TGGTGCACAA GAAGATGAGG 1381 CCCACCATTA AAGATCACTG GTTGAAACAC CCGGGCCTGG CCCAGCTTTG TGTGACCATC 1441 GAGGAGTGCT GGGACCATGA TGCAGAGGCT CGCTTGTCCG CGGGCTGTGT GGAGGAGCGG 1501 GTGTCCCTGA TTCGGAGGTC GGTCAATGGC ACTACCTCGG ACTGTCTCGT TTCCCTGGTG 1561 ACCTCTGTCA CCAATGTGGA CCTGCCCCCT AAAGAGTCAA GCATCTTGCC AACTTTCTTG 1621 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 1681 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 1741 GGAAAGGACG ACGGATGAGT TATGACTAGG AGTGGACGCG TTAAGTCgac aatcaacctc 1801 tggattacaa aatttgtgaa agatt