Construct: ORF TRCN0000475454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008398.1_s317c1
- Derived from:
- ccsbBroadEn_07112
- DNA Barcode:
- CACCAACTTGCCTTTAGTCCAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TXNRD1 (7296)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475454
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7296 | TXNRD1 | thioredoxin reductase 1 | NM_182729.3 | 99.7% | 99.5% | 165C>T;1490G>A;1494A>C |
| 2 | human | 7296 | TXNRD1 | thioredoxin reductase 1 | NM_182742.3 | 99.7% | 99.5% | 165C>T;1490G>A;1494A>C |
| 3 | human | 7296 | TXNRD1 | thioredoxin reductase 1 | NM_182743.3 | 99.7% | 99.5% | 165C>T;1490G>A;1494A>C |
| 4 | human | 7296 | TXNRD1 | thioredoxin reductase 1 | NM_001261446.2 | 92.1% | 91.9% | (many diffs) |
| 5 | human | 7296 | TXNRD1 | thioredoxin reductase 1 | NM_001261445.2 | 90.7% | 90.5% | (many diffs) |
| 6 | human | 7296 | TXNRD1 | thioredoxin reductase 1 | NM_003330.4 | 90.3% | 90.1% | (many diffs) |
| 7 | human | 7296 | TXNRD1 | thioredoxin reductase 1 | NM_001093771.3 | 76.7% | 76.5% | (many diffs) |
| 8 | mouse | 50493 | Txnrd1 | thioredoxin reductase 1 | NM_001042513.1 | 85.5% | 91.1% | (many diffs) |
| 9 | mouse | 50493 | Txnrd1 | thioredoxin reductase 1 | NM_001042514.1 | 85.5% | 91.1% | (many diffs) |
| 10 | mouse | 50493 | Txnrd1 | thioredoxin reductase 1 | NM_015762.2 | 85.5% | 91.1% | (many diffs) |
| 11 | mouse | 50493 | Txnrd1 | thioredoxin reductase 1 | NM_001042523.1 | 69.6% | 74.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1563
- ORF length:
- 1497
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cggccctgaa gatcttccca agtcctatga ctatgacctt atcatcattg 121 gaggtggctc aggaggtctg gcagctgcta aggaggcagc ccaatatggc aagaaggtga 181 tggtcctgga ctttgtcact cccacccctc ttggaactag atggggtctt ggaggaacat 241 gtgtgaatgt gggttgcata cctaaaaaac tgatgcatca agcagctttg ttaggacaag 301 ccctgcaaga ctctcgaaat tatggatgga aagtcgagga gacagttaag catgattggg 361 acagaatgat agaagctgta cagaatcaca ttggctcttt gaattggggc taccgagtag 421 ctctgcggga gaaaaaagtc gtctatgaga atgcttatgg gcaatttatt ggtcctcaca 481 ggattaaggc aacaaataat aaaggcaaag aaaaaattta ttcagcagag agatttctca 541 ttgccactgg tgaaagacca cgttacttgg gcatccctgg tgacaaagaa tactgcatca 601 gcagtgatga tcttttctcc ttgccttact gcccgggtaa gaccctggtt gttggagcat 661 cctatgtcgc tttggagtgc gctggatttc ttgctggtat tggtttagac gtcactgtta 721 tggttaggtc cattcttctt agaggatttg accaggacat ggccaacaaa attggtgaac 781 acatggaaga acatggcatc aagtttataa gacagttcgt accaattaaa gttgaacaaa 841 ttgaagcagg gacaccaggc cgactcagag tagtagctca gtccaccaat agtgaggaaa 901 tcattgaagg agaatataat acggtgatgc tggcaatagg aagagatgct tgcacaagaa 961 aaattggctt agaaaccgta ggggtgaaga taaatgaaaa gactggaaaa atacctgtca 1021 cagatgaaga acagaccaat gtgccttaca tctatgccat tGGCGATATA TTGGAGGATA 1081 AGGTGGAGCT CACCCCAGTT GCAATCCAGG CAGGAAGATT GCTGGCTCAG AGGCTCTATG 1141 CAGGTTCCAC TGTCAAGTGT GACTATGAAA ATGTTCCAAC CACTGTATTT ACTCCTTTGG 1201 AATATGGTGC TTGTGGCCTT TCTGAGGAGA AAGCTGTGGA GAAGTTTGGG GAAGAAAATA 1261 TTGAGGTTTA CCATAGTTAC TTTTGGCCAT TGGAATGGAC GATTCCGTCA AGAGATAACA 1321 ACAAATGTTA TGCAAAAATA ATCTGTAATA CTAAAGACAA TGAACGTGTT GTGGGCTTTC 1381 ACGTACTGGG TCCAAATGCT GGAGAAGTTA CACAAGGCTT TGCAGCTGCG CTCAAATGTG 1441 GACTGACCAA AAAGCAGCTG GACAGCACAA TTGGAATCCA CCCTGTCTGT GCAGAGGTAT 1501 TCACAACATT GTCTGTGACC AAGCGCTCTG GGGCAAGCAT CCTCCAGGCT GGCTACTGCG 1561 GTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1621 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1681 GGCTTTATAT ATCTTGTGGA AAGGACGACA CCAACTTGCC TTTAGTCCAA AGACGCGTTA 1741 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt