Construct: ORF TRCN0000475480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014426.1_s317c1
- Derived from:
- ccsbBroadEn_03955
- DNA Barcode:
- CCCGTTTGCATGGGTATGTAGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH8A1 (64577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64577 | ALDH8A1 | aldehyde dehydrogenase 8 fa... | NM_022568.4 | 100% | 100% | |
| 2 | human | 64577 | ALDH8A1 | aldehyde dehydrogenase 8 fa... | NM_001193480.2 | 89.7% | 89.7% | 441_442ins150 |
| 3 | human | 64577 | ALDH8A1 | aldehyde dehydrogenase 8 fa... | NM_170771.3 | 88.9% | 88.9% | 849_850ins162 |
| 4 | mouse | 237320 | Aldh8a1 | aldehyde dehydrogenase 8 fa... | NM_178713.4 | 85.4% | 89.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1530
- ORF length:
- 1461
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctggaaca aacgcacttt tgatgctgga aaacttcata gatggaaaat 121 ttttaccttg tagctcatat atagattctt acgacccatc aacaggggaa gtgtattgca 181 gagtgccaaa tagtggaaaa gacgagatcg aagccgcggt caaggccgcc agagaagcct 241 ttcccagctg gtcatcccgc agcccccagg agcgctcacg ggtcctgaac caggtggcgg 301 atttgctgga gcagtccctg gaggagtttg cccaggccga gtctaaagac caagggaaaa 361 ccttagcact ggcaagaacc atggacattc cccggtctgt gcagaacttc aggttcttcg 421 cttcctccag cctgcaccac acgtcagagt gcacgcagat ggaccacctg ggctgcatgc 481 actacacggt gcgggccccg gtgggagtcg ctggtctgat cagcccctgg aatttgccac 541 tctacttgct gacctggaag atagctccag cgatggctgc agggaacact gtgatagcca 601 agcccagtga gctgacttca gtgactgcgt ggatgttgtg caaactcctg gataaagcag 661 gtgttccacc aggtgtggtc aatattgtgt ttggaaccgg gcccagggtg ggtgaggccc 721 tggtgtccca cccagaggtg cccctgatct ccttcaccgg gagccagccc accgctgagc 781 ggatcaccca gctgagcgct ccccactgca aaaagctctc cctggagctg gggggcaaga 841 atcctgccat catctttgag gacgccaacc tggatgagtg cattccggca accgtcaggt 901 ccagctttgc caaccagggt gaaatctgtc tctgtaccag caggatcttt gtccagaaga 961 gcatctatag tgaattttta aagagatttg tagaagctac cagaaagtgg aaagtcggca 1021 ttccctctga tccactggtg agcataggtg ctctgataag taaagcacat ttggagaaag 1081 tcagaagtta cgtcaagaga gctcttgctg aaggtgccca aatttggtgc ggtgagggag 1141 tggataagtt gagcctccct gccaggaacc aggcaggcta ctttatgctt cccacggtga 1201 taacagACAT TAAGGATGAA TCCTGCTGCA TGACGGAAGA GATATTTGGT CCAGTGACGT 1261 GTGTCGTCCC CTTTGATAGT GAAGAGGAGG TGATTGAAAG AGCCAACAAC GTTAAGTATG 1321 GGCTGGCGGC TACCGTGTGG TCCAGCAATG TGGGGCGCGT CCACCGGGTG GCTAAGAAGC 1381 TGCAGTCTGG CTTGGTCTGG ACCAACTGCT GGCTCATCAG GGAGCTGAAC CTTCCTTTCG 1441 GGGGGATGAA GAGTTCTGGA ATAGGTAGAG AGGGAGCCAA GGACTCTTAC GACTTCTTCA 1501 CTGAGATCAA AACCATCACC GTTAAACACT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1561 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1621 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCCGT 1681 TTGCATGGGT ATGTAGGTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1741 tgaaagatt