Construct: ORF TRCN0000475486

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008889.1_s317c1
Derived from:
ccsbBroadEn_08968
DNA Barcode:
ACCACTAATACATCGGAACCCTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MFSD13A (79847)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475486
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79847 MFSD13A major facilitator superfami... NM_024789.4 99.8% 100% 204C>T;729A>G
2 human 79847 MFSD13A major facilitator superfami... XM_006717973.3 99.8% 100% 204C>T;729A>G
3 human 79847 MFSD13A major facilitator superfami... XM_006717974.3 99.8% 100% 204C>T;729A>G
4 human 79847 MFSD13A major facilitator superfami... XM_011540160.2 99.8% 100% 204C>T;729A>G
5 human 79847 MFSD13A major facilitator superfami... XM_011540163.2 99.8% 100% 204C>T;729A>G
6 human 79847 MFSD13A major facilitator superfami... XM_017016654.1 99.8% 100% 204C>T;729A>G
7 human 79847 MFSD13A major facilitator superfami... XM_017016655.1 99.8% 100% 204C>T;729A>G
8 human 79847 MFSD13A major facilitator superfami... XM_017016656.1 99.8% 100% 204C>T;729A>G
9 human 79847 MFSD13A major facilitator superfami... XM_017016657.1 85.7% 82.5% (many diffs)
10 human 79847 MFSD13A major facilitator superfami... XM_017016658.2 85.7% 82.5% (many diffs)
11 human 79847 MFSD13A major facilitator superfami... XM_011540167.2 70.6% 70.7% 204C>T;250_251ins453;276A>G
12 human 79847 MFSD13A major facilitator superfami... XM_024448171.1 70.6% 70.7% 204C>T;250_251ins453;276A>G
13 human 79847 MFSD13A major facilitator superfami... XM_017016660.1 57.2% 53.9% (many diffs)
14 human 79847 MFSD13A major facilitator superfami... XM_017016661.1 57.2% 53.9% (many diffs)
15 human 79847 MFSD13A major facilitator superfami... XM_017016662.1 57.2% 53.9% (many diffs)
16 human 79847 MFSD13A major facilitator superfami... XM_024448172.1 57.2% 53.9% (many diffs)
17 human 79847 MFSD13A major facilitator superfami... XM_011540169.3 56.6% 53.8% (many diffs)
18 human 79847 MFSD13A major facilitator superfami... XM_024448173.1 47.5% 47.5% 0_1ins813
19 mouse 75146 Mfsd13a major facilitator superfami... NM_029186.2 86.7% 88.8% (many diffs)
20 mouse 75146 Mfsd13a major facilitator superfami... XR_386522.1 52.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1620
ORF length:
1551
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggctgggt cagccccagg cctggttgct gggtctgccc acagctgtgg
121tctatggctc cctggctctc ttcaccacca tcctgcacaa tgtcttcctg ctctactatg
181tggacacctt tgtctcagtg tacaagatca acaaaatggc cttctgggtc ggagagacag
241tgtttctcct ctggaacagc ctcaatgacc ctctcttcgg ttggctcagt gaccggcagt
301tcctcagctc ccagccccgg tcaggcgccg ggctctcctc aagggctgtg gtgctggccc
361gggtgcaggc cctgggctgg catgggccgc tgctggcgct gtcgttcctg gcgttctggg
421tgccctgggc cccagctggc ctgcagttct tgctgtgcct gtgcctctat gatggcttcc
481tgacgctcgt ggacctgcac caccatgcct tgctggccga cctggccctc tcagcccacg
541accgcaccca cctcaacttc tactgctccc tcttcagcgc ggccggctcc ctctctgtct
601ttgcatccta tgccttttgg aacaaggagg atttctcctc cttccgcgct ttctgcgtga
661cactggctgt cagctctggg ctgggctttc tgggggccac acagctgctg aggcggcggg
721ttgaggcggc ccgaaaggac ccagggtgct caggcctggt tgtggatagc ggcctgtgtg
781gagaggagct gcttgtgggc agtgaggagg cggacagcat caccttgggc cggtatctcc
841ggcagctggc acgccatcgg aacttcctgt ggttcgtgag catggacctg gtgcaggtct
901tccactgcca cttcaacagc aacttcttcc ctctcttcct ggagcatctg ttgtccgacc
961atatctccct ttccacgggc tccatcctgt tgggcctctc ctatgtcgct ccccatctca
1021acaacctcta cttcctgtcc ctgtgccggc gctggggcgt ctacgcggtg gtgcgggggc
1081tcttcctgct caagctggga cttagcctgc tcatgttgtt ggccggcccg gaccacctca
1141gcctgctgtg cctcttcatt gccagcaacc gcgtcttcac tgagggcacc tgtaagctgc
1201tgaccttggt ggtcactgac ctggtagacg aggacctggt gctgaaccac cgcaagcagg
1261cagcctcggc actcctcttt ggcatggttg ccttggtgac caagccaggc cagacctttg
1321ccccgctgcT GGGCACCTGG CTGCTCTGTT TCTACACAGG TCATGACCTC TTCCAGCAGT
1381CCCTCATAAC CCCTGTGGGG AGTGCCCATC CCTGGCCAGA GCCCCCAGCT CCAGCCCCTG
1441CACAGGCCCC GACGCTCCGC CAGGGCTGCT TCTACCTGCT GGTGCTGGTG CCCATCACCT
1501GTGCTCTGCT GCAGCTCTTC ACCTGGTCCC AGTTCACGCT GCATGGGAGA CGCCTGCACA
1561TGGTCAAGGC CCAGCGCCAG AACCTGTCAC AGGCCCAAAC CCTGGATGTT AAGATGGTGT
1621TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1681GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1741TTTATATATC TTGTGGAAAG GACGAACCAC TAATACATCG GAACCCTTTA CGCGTTAAGT
1801Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)