Construct: ORF TRCN0000475486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008889.1_s317c1
- Derived from:
- ccsbBroadEn_08968
- DNA Barcode:
- ACCACTAATACATCGGAACCCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD13A (79847)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79847 | MFSD13A | major facilitator superfami... | NM_024789.4 | 99.8% | 100% | 204C>T;729A>G |
| 2 | human | 79847 | MFSD13A | major facilitator superfami... | XM_006717973.3 | 99.8% | 100% | 204C>T;729A>G |
| 3 | human | 79847 | MFSD13A | major facilitator superfami... | XM_006717974.3 | 99.8% | 100% | 204C>T;729A>G |
| 4 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540160.2 | 99.8% | 100% | 204C>T;729A>G |
| 5 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540163.2 | 99.8% | 100% | 204C>T;729A>G |
| 6 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016654.1 | 99.8% | 100% | 204C>T;729A>G |
| 7 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016655.1 | 99.8% | 100% | 204C>T;729A>G |
| 8 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016656.1 | 99.8% | 100% | 204C>T;729A>G |
| 9 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016657.1 | 85.7% | 82.5% | (many diffs) |
| 10 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016658.2 | 85.7% | 82.5% | (many diffs) |
| 11 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540167.2 | 70.6% | 70.7% | 204C>T;250_251ins453;276A>G |
| 12 | human | 79847 | MFSD13A | major facilitator superfami... | XM_024448171.1 | 70.6% | 70.7% | 204C>T;250_251ins453;276A>G |
| 13 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016660.1 | 57.2% | 53.9% | (many diffs) |
| 14 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016661.1 | 57.2% | 53.9% | (many diffs) |
| 15 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016662.1 | 57.2% | 53.9% | (many diffs) |
| 16 | human | 79847 | MFSD13A | major facilitator superfami... | XM_024448172.1 | 57.2% | 53.9% | (many diffs) |
| 17 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540169.3 | 56.6% | 53.8% | (many diffs) |
| 18 | human | 79847 | MFSD13A | major facilitator superfami... | XM_024448173.1 | 47.5% | 47.5% | 0_1ins813 |
| 19 | mouse | 75146 | Mfsd13a | major facilitator superfami... | NM_029186.2 | 86.7% | 88.8% | (many diffs) |
| 20 | mouse | 75146 | Mfsd13a | major facilitator superfami... | XR_386522.1 | 52.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1620
- ORF length:
- 1551
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggctgggt cagccccagg cctggttgct gggtctgccc acagctgtgg 121 tctatggctc cctggctctc ttcaccacca tcctgcacaa tgtcttcctg ctctactatg 181 tggacacctt tgtctcagtg tacaagatca acaaaatggc cttctgggtc ggagagacag 241 tgtttctcct ctggaacagc ctcaatgacc ctctcttcgg ttggctcagt gaccggcagt 301 tcctcagctc ccagccccgg tcaggcgccg ggctctcctc aagggctgtg gtgctggccc 361 gggtgcaggc cctgggctgg catgggccgc tgctggcgct gtcgttcctg gcgttctggg 421 tgccctgggc cccagctggc ctgcagttct tgctgtgcct gtgcctctat gatggcttcc 481 tgacgctcgt ggacctgcac caccatgcct tgctggccga cctggccctc tcagcccacg 541 accgcaccca cctcaacttc tactgctccc tcttcagcgc ggccggctcc ctctctgtct 601 ttgcatccta tgccttttgg aacaaggagg atttctcctc cttccgcgct ttctgcgtga 661 cactggctgt cagctctggg ctgggctttc tgggggccac acagctgctg aggcggcggg 721 ttgaggcggc ccgaaaggac ccagggtgct caggcctggt tgtggatagc ggcctgtgtg 781 gagaggagct gcttgtgggc agtgaggagg cggacagcat caccttgggc cggtatctcc 841 ggcagctggc acgccatcgg aacttcctgt ggttcgtgag catggacctg gtgcaggtct 901 tccactgcca cttcaacagc aacttcttcc ctctcttcct ggagcatctg ttgtccgacc 961 atatctccct ttccacgggc tccatcctgt tgggcctctc ctatgtcgct ccccatctca 1021 acaacctcta cttcctgtcc ctgtgccggc gctggggcgt ctacgcggtg gtgcgggggc 1081 tcttcctgct caagctggga cttagcctgc tcatgttgtt ggccggcccg gaccacctca 1141 gcctgctgtg cctcttcatt gccagcaacc gcgtcttcac tgagggcacc tgtaagctgc 1201 tgaccttggt ggtcactgac ctggtagacg aggacctggt gctgaaccac cgcaagcagg 1261 cagcctcggc actcctcttt ggcatggttg ccttggtgac caagccaggc cagacctttg 1321 ccccgctgcT GGGCACCTGG CTGCTCTGTT TCTACACAGG TCATGACCTC TTCCAGCAGT 1381 CCCTCATAAC CCCTGTGGGG AGTGCCCATC CCTGGCCAGA GCCCCCAGCT CCAGCCCCTG 1441 CACAGGCCCC GACGCTCCGC CAGGGCTGCT TCTACCTGCT GGTGCTGGTG CCCATCACCT 1501 GTGCTCTGCT GCAGCTCTTC ACCTGGTCCC AGTTCACGCT GCATGGGAGA CGCCTGCACA 1561 TGGTCAAGGC CCAGCGCCAG AACCTGTCAC AGGCCCAAAC CCTGGATGTT AAGATGGTGT 1621 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1681 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1741 TTTATATATC TTGTGGAAAG GACGAACCAC TAATACATCG GAACCCTTTA CGCGTTAAGT 1801 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt