Construct: ORF TRCN0000475488
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004121.1_s317c1
- Derived from:
- ccsbBroadEn_00735
- DNA Barcode:
- CCGCGAATTTATTCCGGCGTGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFHR2 (3080)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475488
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3080 | CFHR2 | complement factor H related 2 | NM_005666.4 | 100% | 100% | |
| 2 | human | 3080 | CFHR2 | complement factor H related 2 | XM_011509458.2 | 98.5% | 98.1% | 418_419insGCAGGTCCACTA |
| 3 | human | 3080 | CFHR2 | complement factor H related 2 | XM_011509459.2 | 92.5% | 92.5% | 0_1ins60 |
| 4 | human | 3080 | CFHR2 | complement factor H related 2 | XM_017001109.1 | 78.1% | 78.1% | 252_253ins177 |
| 5 | human | 3080 | CFHR2 | complement factor H related 2 | XM_005245113.3 | 75.9% | 75.5% | 58_59ins195 |
| 6 | human | 3078 | CFHR1 | complement factor H related 1 | NM_002113.2 | 75.6% | 70.9% | (many diffs) |
| 7 | human | 3080 | CFHR2 | complement factor H related 2 | XM_011509460.2 | 74.4% | 73.7% | 58_59ins195;223_224insGCAGGTCCACTA |
| 8 | human | 3078 | CFHR1 | complement factor H related 1 | XM_011509457.2 | 56.7% | 51.5% | (many diffs) |
| 9 | human | 3080 | CFHR2 | complement factor H related 2 | NM_001312672.1 | 54% | 53.7% | 58_59ins372 |
| 10 | human | 81494 | CFHR5 | complement factor H related 5 | NM_030787.4 | 39.4% | 34.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 876
- ORF length:
- 810
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gctcctggtc agtgtaattc taatctcacg gatatcctct gttgggggag 121 aagcaatgtt ctgtgatttt ccaaaaataa accatggaat tctatatgat gaagaaaaat 181 ataagccatt ttcccaagtt cctacagggg aagttttcta ttactcctgt gaatataatt 241 ttgtgtctcc ttcaaaatcc ttttggactc gcataacgtg cgcagaagaa ggatggtcac 301 caacaccaaa gtgtctcaga ctgtgtttct ttccttttgt ggaaaatggt cattctgaat 361 cttcaggaca aacacatctg gaaggtgata ctgtacaaat tatttgcaac acaggataca 421 gacttcaaaa caatgagaac aacatttcat gtgtagaacg gggctggtcc acTCCTCCCA 481 AATGCAGGTC CACTATTTCT GCAGAAAAAT GTGGGCCCCC TCCACCTATT GACAATGGAG 541 ACATTACTTC ATTCCTGTTG TCAGTATATG CTCCAGGTTC ATCAGTTGAG TACCAGTGCC 601 AGAACTTGTA TCAACTTGAG GGTAACAATC AAATAACATG TAGAAACGGA CAATGGTCAG 661 AACCACCAAA ATGCTTAGAT CCATGTGTAA TATCACAAGA AATTATGGAA AAATATAACA 721 TAAAATTAAA GTGGACAAAC CAACAAAAGC TTTATTCAAG AACAGGTGAC ATAGTTGAAT 781 TTGTTTGTAA ATCTGGATAT CATCCAACAA AATCTCATTC ATTTCGAGCA ATGTGTCAGA 841 ATGGGAAACT GGTATATCCC AGTTGTGAAG AAAAATACCC AACTTTCTTG TACAAAGTGG 901 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 961 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1021 ACCGCGAATT TATTCCGGCG TGAGAACGCG TTAAGTCgac aatcaacctc tggattacaa 1081 aatttgtgaa agatt