Construct: ORF TRCN0000475511
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001918.1_s317c1
- Derived from:
- ccsbBroadEn_10386
- DNA Barcode:
- ATATGGTCGGTGCGGGTATATATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HERC2P9 (440248)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475511
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440248 | HERC2P9 | hect domain and RLD 2 pseud... | NR_036443.1 | 43.1% | 1_216del;1495_2962del | |
| 2 | human | 400322 | HERC2P2 | hect domain and RLD 2 pseud... | NR_002824.3 | 20.5% | (many diffs) | |
| 3 | human | 283755 | HERC2P3 | hect domain and RLD 2 pseud... | NR_036432.1 | 17.7% | (many diffs) | |
| 4 | human | 8924 | HERC2 | HECT and RLD domain contain... | XR_001751410.1 | 14.2% | (many diffs) | |
| 5 | human | 8924 | HERC2 | HECT and RLD domain contain... | XM_017022697.1 | 13.1% | 11.8% | (many diffs) |
| 6 | human | 8924 | HERC2 | HECT and RLD domain contain... | XM_017022698.1 | 13.1% | 11.8% | (many diffs) |
| 7 | human | 8924 | HERC2 | HECT and RLD domain contain... | XR_931930.2 | 12.3% | (many diffs) | |
| 8 | human | 8924 | HERC2 | HECT and RLD domain contain... | XM_006720727.3 | 8.7% | 8% | (many diffs) |
| 9 | human | 8924 | HERC2 | HECT and RLD domain contain... | XM_005268276.5 | 8.6% | 7.9% | (many diffs) |
| 10 | human | 8924 | HERC2 | HECT and RLD domain contain... | XM_017022695.1 | 8.6% | 7.9% | (many diffs) |
| 11 | human | 8924 | HERC2 | HECT and RLD domain contain... | XM_017022696.1 | 8.6% | 7.9% | (many diffs) |
| 12 | human | 8924 | HERC2 | HECT and RLD domain contain... | XM_006720726.3 | 8.6% | 7.8% | (many diffs) |
| 13 | human | 8924 | HERC2 | HECT and RLD domain contain... | NM_004667.5 | 8.6% | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtcctggct gtggttggag gcatcgatgg tcgcctgcgc ctgggtggcc 121 aagttgtgca cgatgacttt ggagaagtca ccatgactcg catcaccctg aagggcaaaa 181 tcaccgtgca gttctctgac atgcggacgt gtcgcgtttg cccattgaat cagctgaaac 241 cactccctgc cgtggccttt aatgtgaaca acctgccctt cacagagccc atgctgtctg 301 tctgggctca gttggtgaac ctcgctggaa gcaagttaga aaagcacaaa ataaagaaat 361 cgactaaaca ggcctttgca ggacaagtgg acctggacct gctgcggtgc cagcagttga 421 agctatacat cctgaaagca ggtcgggcgc tgttctccca ccaggataaa ctgcggcaga 481 tcctgtctca gccagctgtt caggagactg gaagtgttca cacagatgat ggagcagtgg 541 tatcacctga ccttggggac atgtctcctg aagggccgca gccccccatg atcctcttgc 601 agcagctgct ggcctcggcc acccagccgt ctcctgtgaa ggccatattt gataaacagg 661 aacttgaggc tgctgcactg gccgtttgcc agtgcttggc tgtggagtcc actcaccctt 721 cgagcccagg atttgaagac tgcagctcca gtgaggccac cacgcctgtc gccgtgcagc 781 acatccgccc tgccagagtg aagaggcgca agcagtcgcc cgttcccgct ctgccgatcg 841 tggtgcagct catggagatg ggattttcca gaaggaacat cgagtttgcc ctGAAGTCTC 901 TCACTGGTGC TTCCGGGAAT GCGTCCGGCT TGCCTGGTGT GGAAGCCTTG GTCGGGTGGC 961 TGCTGGACCA CTCCGACATA CAGGTCACGG AGCTCTCAGA TGCAGACACG GTGTCCGACG 1021 AGTATTCTGA CGAGGAGGTG GTGGAGGACG TGGATGATGC CGCCTACTCC ATGTCTACTG 1081 GTGCTGTTGT GACGGAGAGC CAGACGTACA AAAACCGAGC TGGTTTCTTG GGTAATGATG 1141 ATTATGCTGT ATATGTGAGA GAGAATATTC AGGTGGGAAT GATGGTTAGA TGCTGCCGAA 1201 CATACGAAGA AGTGTGCGAA GGTGATGTGA TGTTGGCAAA GTCATCAAGC TGGACAGAGA 1261 TGGATTGCAT GATCTCAATG TGCAGTGTGA CTGGCAGCAG AAAGGGGGCA TCTACTGGTT 1321 TAGGTACATT CATGTGGAAC TTATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TATGGTCGGT 1501 GCGGGTATAT ATAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att