Construct: ORF TRCN0000475566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001470.1_s317c1
- Derived from:
- ccsbBroadEn_11633
- DNA Barcode:
- CTAACCTAATGTCGGTTCTATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPM1E (22843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22843 | PPM1E | protein phosphatase, Mg2+/M... | NM_014906.5 | 99.7% | 99.7% | 99_100insGAACCC |
| 2 | human | 22843 | PPM1E | protein phosphatase, Mg2+/M... | XM_017024359.1 | 68.1% | 68.1% | 0_1ins723 |
| 3 | human | 22843 | PPM1E | protein phosphatase, Mg2+/M... | XM_024450657.1 | 68.1% | 68.1% | 0_1ins723 |
| 4 | human | 22843 | PPM1E | protein phosphatase, Mg2+/M... | NR_048561.1 | 31.5% | (many diffs) | |
| 5 | mouse | 320472 | Ppm1e | protein phosphatase 1E (PP2... | NM_177167.4 | 87.8% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2340
- ORF length:
- 2271
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccggctgc atccctgagg agaaaactta ccggcgcttc ctggagctat 121 tcctgggcga gtttcgcgga ccgtgcggcg gcggcgagcc ggagccggaa cccgaacccg 181 aacccgaacc cgaacccgaa cccgagtccg agcccgagcc cgaacctgaa ctggtagaag 241 ctgaggcggc cgaggcttcg gtagaggaac ccggggagga ggcggccacg gtagccgcga 301 cggaggaggg ggaccaggag caagacccgg agcccgagga ggaggcggcg gttgagggtg 361 aggaggagga ggagggcgcg gcgacggcgg cggcagcccc ggggcactcg gccgtgccgc 421 cgccgccgcc ccagctgccg cctttgcccc cgctcccgcg accgctgtca gagcgcatca 481 cccgcgagga ggtggagggc gaaagcctgg acctgtgcct gcagcagctc tacaaatata 541 attgcccttc ctttttggct gctgctttag ccagagccac atcagatgaa gtccttcaga 601 gtgatctttc tgcacattat atcccaaagg aaacggatgg cacagaaggg actgtggaga 661 ttgagacagt gaaattggcc cgttctgtct tcagcaaact acacgagatt tgctgcagct 721 gggtgaaaga cttccccctc cgcaggagac cccagcttta ttatgagaca tcaatccatg 781 ccatcaaaaa catgcgcagg aaaatggagg acaaacatgt ctgcattcct gactttaata 841 tgctcttcaa cctagaggac caggaagaac aagcttactt tgcagtgttt gatggccatg 901 ggggagtaga tgctgctatt tatgcctcca ttcacctcca cgttaactta gtccgccagg 961 agatgttccc ccatgatcct gctgaggccc tgtgcagggc cttccgggtc actgatgagc 1021 ggtttgtgca gaaagcagcc agggagagct taagatgtgg gaccacagga gtggtgactt 1081 tcatcagagg caacatgcta catgtggcct gggtgggtga ttcccaggtt atgcttgtga 1141 gaaagggcca agctgttgaa ctaatgaagc cacacaaacc agacagagag gatgaaaagc 1201 agagaattga ggcccttgga ggttgcgtag tctggtttgg tgcctggagg gtgaatggaa 1261 gtctgtcggt ttccagagct attggagatg ctgaacataa gccatatatc tgtggggatg 1321 cagattctgc ctccactgtt ctggatggga ccgaagacta cctcattctg gcctgtgatg 1381 gcttctatga caccgtgaac cctgatgagg cagtgaaagt tgtgtccgac cacctgaaag 1441 agaataatgg agacagcagc atggttgccc acaaattagt ggcatcagct cgtgatgctg 1501 ggtcaagtga taacatcacg gttattgtgg tattcctgag ggacatgaac aaagctgtaa 1561 atgttagtga ggaatcagat tggacagaga actcttttca aggagggcaa gaagatggtg 1621 gggatgataa ggagaatcat ggagagtgca aacgcccttg gcctcagcac cagtgctcag 1681 caccagccga cctaggctat gatgggcgtg tggattcatt cactgataga actagcctga 1741 gcccagggtc ccaaatcaac gtgctggaag acccaggcta cctagatctc acacaaatag 1801 aagcaagcaa acctcacagt gcccagtttt tgctaccagt tgagatgttt ggtcctggtg 1861 caccaaagaa agcaaatctt attaatgagt taatgatgga gaaaaaatca gttcagtcat 1921 cattgcctga atggagtggt gctggagagt ttcccactgc tttcaatttg ggttcaacag 1981 gggagcagat atacagaatg cagagcttgt ctcctgtctg ttcagggttg gaaaatgaac 2041 agttcaaaTC CCCGGGAAAC AGAGTTTCTA GATTGTCTCA TTTACGCCAC CACTACTCAA 2101 AGAAGTGGCA CAGATTCAGG TTTAATCCAA AGTTTTATTC ATTTCTCTCT GCTCAAGAGC 2161 CTTCCCACAA AATAGGCACT AGCCTGTCCT CACTTACTGG AAGTGGGAAG AGAAATAGGA 2221 TAAGAAGTTC TCTGCCATGG AGGCAAAATA GTTGGAAAGG GTACAGTGAA AACATGAGGA 2281 AGCTCAGAAA GACTCATGAT ATTCCATGCC CAGATCTTCC TTGGAGCTAT AAAATAGAAT 2341 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2401 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2461 TTTATATATC TTGTGGAAAG GACGACTAAC CTAATGTCGG TTCTATATTA CGCGTTAAGT 2521 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt