Construct: ORF TRCN0000475628
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011565.1_s317c1
- Derived from:
- ccsbBroadEn_09998
- DNA Barcode:
- TCAATGGATGGTACCGACACTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP19-4 (337971)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475628
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 337971 | KRTAP19-4 | keratin associated protein ... | NM_181610.3 | 99.6% | 98.8% | 143A>G |
| 2 | human | 337974 | KRTAP19-7 | keratin associated protein ... | NM_181614.2 | 64.5% | 58.6% | (many diffs) |
| 3 | human | 337973 | KRTAP19-6 | keratin associated protein ... | NM_181612.3 | 61.1% | 54.7% | (many diffs) |
| 4 | human | 337972 | KRTAP19-5 | keratin associated protein ... | NM_181611.3 | 58.3% | 53.7% | (many diffs) |
| 5 | human | 337970 | KRTAP19-3 | keratin associated protein ... | NM_181609.4 | 56.8% | 53.9% | (many diffs) |
| 6 | human | 337882 | KRTAP19-1 | keratin associated protein ... | NM_181607.3 | 49.2% | 45.9% | (many diffs) |
| 7 | mouse | 170654 | Krtap19-4 | keratin associated protein ... | NM_130873.1 | 51.8% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 345
- ORF length:
- 252
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAGCTA CTATGGCAGC TATTACAGAG 121 GCCTGGGCTA TGGCTGTGGA GGCTTTGGTG GCCTAGGCTA TGGCTATGGC TGTGGATGTG 181 GCAGCTTCCG CAGACTGGGT TATGGCTGTG GCTTTGGAGG CAACGGATAT GGCTGCTGCC 241 GCCCATCATG CTATGGAGGA TATGGATTCT CAATTCTACT GAAATCCTAC CCTGAGGACA 301 CGATATCAGA GGTCATAAGA AGATCATTCA ATTTAACCAA ATATTTGCCA ACTTTCTTGT 361 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 421 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA TCAATGGATG GTACCGACAC TTTGACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt