Construct: ORF TRCN0000475654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008707.1_s317c1
- Derived from:
- ccsbBroadEn_11769
- DNA Barcode:
- GGCCGTGCTGTTACGGCAGAGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAFA5 (25817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25817 | TAFA5 | TAFA chemokine like family ... | NM_001082967.3 | 86.3% | 86.3% | 1_54del |
| 2 | human | 25817 | TAFA5 | TAFA chemokine like family ... | NM_015381.7 | 84.8% | 78.7% | (many diffs) |
| 3 | mouse | 106014 | Fam19a5 | family with sequence simila... | NM_001252310.1 | 79.2% | 86.3% | (many diffs) |
| 4 | mouse | 106014 | Fam19a5 | family with sequence simila... | NM_134096.2 | 79% | 78.7% | (many diffs) |
| 5 | mouse | 106014 | Fam19a5 | family with sequence simila... | XM_006520260.2 | 48.3% | 52.5% | (many diffs) |
| 6 | mouse | 106014 | Fam19a5 | family with sequence simila... | XM_006520261.2 | 47.4% | 47.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 411
- ORF length:
- 342
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcctcaact ttctgggcgt tcatgatcct ggccagcctg ctcatcgccT 121 ACTGCAGTCA GCTGGCCGCC GGCACCTGTG AGATTGTGAC CTTGGACCGG GACAGCAGCC 181 AGCCTCGGAG GACGATCGCC CGGCAGACCG CCCGCTGTGC GTGTAGAAAG GGGCAGATCG 241 CCGGCACCAC GAGAGCCCGG CCCGCCTGTG TGGACGCAAG AATCATCAAG ACCAAGCAGT 301 GGTGTGACAT GCTTCCGTGT CTGGAGGGGG AAGGCTGCGA CTTGTTAATC AACCGGTCAG 361 GCTGGACGTG CACGCAGCCC GGCGGGAGGA TAAAGACCAC CACGGTCTCC TTGCCAACTT 421 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 481 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 541 CTTGTGGAAA GGACGAGGCC GTGCTGTTAC GGCAGAGCGA ACGCGTTAAG TCgacaatca 601 acctctggat tacaaaattt gtgaaagatt