Transcript: Mouse NM_001252310.1

Mus musculus family with sequence similarity 19, member A5 (Fam19a5), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Fam19a5 (106014)
Length:
2665
CDS:
194..592

Additional Resources:

NCBI RefSeq record:
NM_001252310.1
NBCI Gene record:
Fam19a5 (106014)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001252310.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000283025 TTGTGTGGACGCTCGAATTAT pLKO_005 445 CDS 100% 15.000 21.000 N Fam19a5 n/a
2 TRCN0000201896 CTTGTGTGGACGCTCGAATTA pLKO.1 444 CDS 100% 13.200 18.480 N Fam19a5 n/a
3 TRCN0000217391 GACCAACTCTATCGTACTAAA pLKO.1 1335 3UTR 100% 13.200 18.480 N Fam19a5 n/a
4 TRCN0000264393 TGACTATCATGCTAGTAATAT pLKO_005 2154 3UTR 100% 15.000 10.500 N Fam19a5 n/a
5 TRCN0000190105 GACAAAGCAGTGGTGTGACAT pLKO.1 469 CDS 100% 4.950 3.465 N Fam19a5 n/a
6 TRCN0000264391 TGTGAGATTGTGACCCTAGAC pLKO_005 326 CDS 100% 4.050 2.835 N Fam19a5 n/a
7 TRCN0000200727 GAATTATCAAGACAAAGCAGT pLKO.1 459 CDS 100% 2.640 1.848 N Fam19a5 n/a
8 TRCN0000264392 GAAGGCTGTGACTTGTTAATC pLKO_005 509 CDS 100% 13.200 7.920 N Fam19a5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001252310.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11769 pDONR223 100% 79.2% 86.3% None (many diffs) n/a
2 ccsbBroad304_11769 pLX_304 0% 79.2% 86.3% V5 (many diffs) n/a
3 TRCN0000475654 GGCCGTGCTGTTACGGCAGAGCGA pLX_317 82.7% 79.2% 86.3% V5 (many diffs) n/a
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