Construct: ORF TRCN0000475671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002415.1_s317c1
- Derived from:
- ccsbBroadEn_00756
- DNA Barcode:
- TGGTATTTCCTAATAACTATATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGCS2 (3158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475671
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3158 | HMGCS2 | 3-hydroxy-3-methylglutaryl-... | NM_005518.4 | 100% | 100% | |
| 2 | human | 3158 | HMGCS2 | 3-hydroxy-3-methylglutaryl-... | NM_001166107.1 | 91.7% | 91.7% | 558_559ins126 |
| 3 | human | 3158 | HMGCS2 | 3-hydroxy-3-methylglutaryl-... | XM_011541313.2 | 89.1% | 89.1% | 683_684ins165 |
| 4 | mouse | 15360 | Hmgcs2 | 3-hydroxy-3-methylglutaryl-... | NM_008256.4 | 86.3% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gcgtctgttg actccagtga agcgcattct gcaactgaca agagcggtgc 121 aggaaacctc cctcacacct gctcgcctgc tcccagtagc ccaccaaagg ttttctacag 181 cctctgctgt ccccctggcc aaaacagata cttggccaaa ggacgtgggc atcctggccc 241 tggaggtcta cttcccagcc caatatgtgg accaaactga cctggagaag tataacaatg 301 tggaagcagg aaagtataca gtgggcttgg gccagacccg tatgggcttc tgctcagtcc 361 aagaggacat caactccctg tgcctgacgg tggtgcaacg gctgatggag cgcatacagc 421 tcccatggga ctctgtgggc aggctggaag taggcactga gaccatcatt gacaagtcca 481 aagctgtcaa aacagtgctc atggaactct tccaggattc aggcaatact gatattgagg 541 gcatagatac caccaatgcc tgctacggtg gtactgcctc cctcttcaat gctgccaact 601 ggatggagtc cagttcctgg gatggtcgtt atgccatggt ggtctgtgga gacattgccg 661 tctatcccag tggtaatgct cgtcccacag gtggggccgg agctgtggct atgctgattg 721 ggcccaaggc ccctctggcc ctggagcgag ggctgagggg aacccatatg gagaatgtgt 781 atgacttcta caaaccaaat ttggcctcgg agtacccaat agtggatggg aagctttcca 841 tccagtgcta cttgcgggcc ttggatcgat gttacacatc ataccgtaaa aaaatccaga 901 atcagtggaa gcaagctggc agcgatcgac ccttcaccct tgacgattta cagtacatga 961 tctttcatac acccttttgc aagatggtcc agaagtctct ggctcgcctg atgttcaatg 1021 acttcctgtc agccagcagt gacacacaaa ccagcttata taaggggctg gaggctttcg 1081 gggggctaaa gctggaagac acctacacca acaaggacct ggataaagca cttctaaagg 1141 cctctcagga catgttcgac aagaaaacca aggcttccct ttacctctcc actcacaatg 1201 ggaacatgta cacctcatcc ctgtacgggt gcctggcctc gcttctgtcc caccactctg 1261 cccaagaact ggctggctcc aggattggtg ccttctctta tggctctggt ttagcagcaa 1321 gtttcttttc atttcgagta tcccaggatg ctgctccagg ctctcccctg gacaagttgg 1381 tgtccagcac atcagacctg ccaaaacgcc tagcctcccg aaagtgtgtg tctcctgagg 1441 agttcacaga aataatgaac caaagagagc aatTCTACCA TAAGGTGAAT TTCTCCCCAC 1501 CTGGTGACAC AAACAGCCTT TTCCCAGGTA CTTGGTACCT GGAGCGAGTG GACGAGCAGC 1561 ATCGCCGAAA GTATGCCCGG CGTCCCGTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGGTA 1741 TTTCCTAATA ACTATATATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt